Ameliorative effect of hesperidin on streptozotocin‐diabetes mellitus‐induced testicular DNA damage and sperm quality degradation in Sprague–Dawley rats

Aksu, Emrah Hicazi; Kandemir, Fatih Mehmet; Küçükler, Sefa

doi:10.1111/jfbc.13938

Cited by 24 publications

(21 citation statements)

References 36 publications

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“…Chronic insulin deficiency leads to the breakdown of fats and structural proteins as substitutes for carbohydrates as an energy source, resulting to organ and body weight loss (Abdel‐Mageid, Abou‐Salem, Salaam, & El‐Garhy, 2018). Our findings showed that diabetic rats had a significant reduction in body and testis weight that are consistent with other experiments (Aksu, Kandemir, & Küçükler, 2021; Makinde, Radenahmad, Adekoya, & Olatunji, 2020). However, restoring blood glucose closer to control level and improving the body and testis weight of animals treated with plants, especially with the combination form, revealed an improvement in metabolic status.…”

Section: Discussionsupporting

confidence: 92%

“…GPx uses GSH as an electron source to reduce H 2 O 2 into water (Aitken & Roman, 2009). Our results illustrated that CAT and GPx activity were substantially reduced in the diabetic group, as confirmed by another study (Aksu et al, 2021;Nna et al, 2019). Also, in diabetic rats, GSSG increased, and GSH decreased significantly, which is consistent with previous studies (Adedara et al, 2019;Zhou, 2020).…”

Section: Discussionsupporting

confidence: 92%

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Synergistic properties of garlic and Citrullus colocynthis on reproductive injury caused by diabetes in male rats: Structural and molecular evidence

Mohammadzadeh

Gol

2022

Journal of Food Biochemistry

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This study evaluates the synergistic effect of garlic and Citrullus colocynthis on diabetic reproductive damage by suppressing the AGEs/RAGE/Nox‐4 signaling pathway. Thirty‐five male Wistar rats were divided into five groups (n = 7/group): Control, Diabetic, Diabetic+G (Garlic, 1 mL/100 g b.w), Diabetic+C (C. colocynthis, 10 mg/kg b.w) and Diabetic+GC (Garlic, 1 mL/100 g b.w and C. colocynthis, 10 mg/kg b.w) groups. At the end of the experimental period (30 days), in diabetic rats, glucose increased, and body & testis weight, luteinizing hormone (LH) and testosterone levels, and sperm count decreased significantly and histopathological injuries were observed. In addition, they have increased testicular apoptosis and oxidative stress. Also, the mechanism based on advanced glycation end products (AGEs)/receptors for advanced glycation end products (RAGE)/NADPH oxidase‐4 (Nox‐4) was activated in diabetic rats. Separate consumption of garlic and C. colocynthis in Diabetic+G and Diabetic+C groups alleviated the negative adverse effect of diabetes to some extent, but when they were used in the combination form (Diabetic+GC) improvement was profound. Testis histopathology, increased body and testis weight, and enhanced capacity in protecting diabetic reproductive injury was seen. Decreases in testosterone and LH concentration and sperm count in diabetic rats were also reversed by combined administration of garlic and C. colocynthis. It regulated oxidative stress markers, meanwhile reducing caspase‐3 immunoexpression. In addition, overexpression of RAGE, Nox‐4 and nuclear transcription factor‐κB (NF‐κB) was inhibited by the combination of garlic and C. colocynthis. Practical Applications Diabetes mellitus is wide spread all around the world with variety of complications in body including reproductive system in which patients suffer from physical and psychological aspects. Despite many efforts in providing agents for controlling diabetes and its complications, economic conditions of some countries make it difficult for people to provide costly medicine and as a result, they have to bear the complications until they pass away. However, traditional medicine is still finding its way, especially in poor countries with emphasis on medicinal plants. There have been many studies on plants to alleviate diabetes or its side effects. But, using one plant for long term, may be not so effective. Here, we attempted to find whether two plants from two different species can show more efficacy than each one alone. We noticed garlic and Citrullus colocynthis despite having beneficial effects when used alone, they could show synergistic effects in combination.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Synergistic properties of garlic and Citrullus colocynthis on reproductive injury caused by diabetes in male rats: Structural and molecular evidence

Mohammadzadeh

Gol

2022

Journal of Food Biochemistry

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“…Flavonoids, which are secondary metabolites of plants, are phenolic compounds found in almost all vegetables and fruits 18–21 . Flavonoids are compounds with various biological and pharmacological properties, including anti‐allergic, vasodilator, anti‐inflammatory, antiapoptotic, antioxidant, and antithrombotic 22–24 . Quercetin ([3,3′,4′,5,7‐pentahydroxyflavone], QUE) is widely found in vegetables and fruits such as broccoli, strawberries, onions, coriander and apples 25,26 .…”

Section: Introductionmentioning

confidence: 99%

“…[18][19][20][21] Flavonoids are compounds with various biological and pharmacological properties, including anti-allergic, vasodilator, anti-inflammatory, antiapoptotic, antioxidant, and antithrombotic. [22][23][24] Quercetin ([3,3 0 ,4 0 ,5,7-pentahydroxyflavone], QUE) is widely found in vegetables and fruits such as broccoli, strawberries, onions, coriander and apples. 25,26 It has taken significant attention among researchers in recent years due to its features like cytoprotective, anti-nociceptive, antioxidant, antithrombotic, anti-carcinogenic and anti-inflammatory properties.…”

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confidence: 99%

Evaluation of protective effects of quercetin against cypermethrin‐induced lung toxicity in rats via oxidative stress, inflammation, apoptosis, autophagy, and endoplasmic reticulum stress pathway

İleritürk

Kandemir

2022

Environmental Toxicology

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Cypermethrin (CYP), a type II synthetic pyrethroid, is the most widely used insecticide worldwide. Inhalation of it may cause side effects. This study is aimed to examine potential protection of quercetin (QUE) which is a well-known antioxidant in CYPinduced lung toxicity. Accordingly, 35 Spraque Dawley male rats were divided into five equal groups as follows: I-Control group, II-QUE group (50 mg/kg/b.w. QUE), III-CYP group (25 mg/kg/b.w. CYP), IV-CYP + QUE 25 (25 mg/kg/b.w. CYP + 25 mg/ kg/b.w. QUE), V-CYP + QUE (25 mg/kg/b.w. CYP + 50 mg/kg/b.w. QUE) were treated with oral gavage throughout 28 days. CYP intoxication was associated with increased malondialdehyde level while glutathione concentration, activities of glutathione peroxidase, superoxide dismutase, and catalase reduced. CYP adminisitration caused of apoptosis in the lung by up-regulating caspase-3 and Bax levels and down-regulating Bcl-2. CYP also caused of endoplasmic reticulum (ER) stress by increasing mRNA transcript levels of PERK, IRE1, ATF6, and GRP78. Additionally, it was observed that CYP administration activated IL-6/JAK2/STAT3/MAPK14 signaling pathway and levels of IL-1β, NF-κB, TNF-α, and iNOS in the lung tissue. Therefore, it was determined that CYP administration triggered autophagy by upregulating LC3A and LC3B mRNA transcript levels. Moreover, the protein levels of NF-κB, caspase-3, Bax, Bcl-2, and cytochorme-c were examined by Western blot analysis. However, cotreatment with QUE at a dose of 25 and 50 mg/kg considerably protective oxidative stress, inflammation, apoptosis, ER stress, autophagy, and IL-6/JAK2/STAT3/MAPK14 signaling pathway in lung tissue. Overall, the findings of this study suggest that lung damage associated with CYP toxicity could be protected by QUE administration.

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“…Previous studies have demonstrated that metabolic syndrome reduces the total plasma testosterone levels, which in turn impairs sperm production and motility after 12 weeks of a high-fat diet [ 25 ]. Moreover, diabetes significantly affects several sperm parameters, including increased testicular DNA damage and sperm degradation due to alternations in glucose transport and increased oxidative stress [ 26 , 27 ]. In diabetic men, hyperglycemia increases oxidative stress, a common pathology seen in approximately 50% of all infertile men, thereby causing infertility [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Effect of High-Fructose Diet-Induced Metabolic Syndrome on the Pituitary-Gonadal Axis in Male Rats

et al. 2022

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Plasma testosterone levels have been found to decrease in older insulin-resistant male patients. Both lower total testosterone levels and a higher incidence of metabolic syndrome have also been reported. The aim of this study was to investigate the effects of high-fructose diet-induced diabetes on both the testosterone release by Leydig cells and the activity of the hypothalamus–pituitary–gonadal (HPG) axis in male rats. Male rats were fed with either standard chow (control group) or a high-fructose diet (fructose-fed group) for 21 weeks. Hyperglycemia, hyperinsulinemia, and hypertension were observed in the fructose-fed group. Moreover, plasma testosterone and LH levels decreased in the fructose-fed group compared to the control group. Sperm motility was also reduced by 15% in the fructose-fed rats. In contrast, the basal release of testosterone from rat Leydig cells was not altered by fructose feeding. Moreover, in vitro studies showed that the testosterone release, in response to different stimulants, including forskolin (an adenylyl cyclase activator, 10−5 M), 8-Br-cAMP (a permeable analog of cAMP, 10−5 M), A23187 (a calcium ionophore, 10−5 M), or 25-hydroxy-cholesterol (water-soluble cholesterol, 10−5 M), did not significantly differ between the fructose-fed and control groups. Interestingly, the release of testosterone in response to human chorionic gonadotropin (hCG, 0.05 IU/mL) was enhanced by eightfold in the control group, but elevenfold in the fructose-fed group. LH receptor expression in rat Leydig cells was also increased. Moreover, LH secretion from the anterior pituitary was altered in the fructose diet-fed group. These results suggest that fructose diet-fed rats have lower plasma testosterone levels, which can lead to a higher sensitivity of hCG in Leydig cells.

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Ameliorative effect of hesperidin on streptozotocin‐diabetes mellitus‐induced testicular DNA damage and sperm quality degradation in Sprague–Dawley rats

Cited by 24 publications

References 36 publications

Synergistic properties of garlic and Citrullus colocynthis on reproductive injury caused by diabetes in male rats: Structural and molecular evidence

Synergistic properties of garlic and Citrullus colocynthis on reproductive injury caused by diabetes in male rats: Structural and molecular evidence

Evaluation of protective effects of quercetin against cypermethrin‐induced lung toxicity in rats via oxidative stress, inflammation, apoptosis, autophagy, and endoplasmic reticulum stress pathway

Effect of High-Fructose Diet-Induced Metabolic Syndrome on the Pituitary-Gonadal Axis in Male Rats

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