Ameliorative Effect of Heat-Killed Lactobacillus plantarum L.137 and/or Aloe vera against Colitis in Mice

Abstract

Inflammatory bowel disease (IBD) is one of the predominant intestinal diseases associated with chronic inflammation and ulceration of the colon. This study explored the ameliorative effect of Aloe vera extract (Aloe) and/or heat-killed Lactobacillus plantarum L.137 (HK L.137) on dextran sodium sulfate (DSS)-induced colitis in mice. Aloe and/or HK L.137 were supplied for 9 days and the mice were challenged with DSS for 7 days. The DSS group demonstrated bloody diarrhea, colitis of high histologic grade, increased nuclear factor-kappa B (NF-κB) p65, inducible nitric oxide synthase (iNOS), myeloperoxidase (MPO), interleukin (IL)-6, and tumor necrosis factor (TNF)-α, and decreased IL-10 expression. These alterations were dwindled in DSS-induced mice treated with Aloe and HK L.137 separately. Aloe and HK L.137 together have augmented the therapeutic effect of each other. In conclusion, our findings demonstrated that Aloe and/or HK L.137 ameliorated DSS-induced colitis by promoting the secretion of anti-inflammatory cytokines and suppressing pro-inflammatory mediators. This study indicated that A. vera may function synergistically with HK L.137 to confer an effective strategy to prevent colitis.

A Novel Role of Dapagliflozin in Mitigation of Acetic Acid-Induced Ulcerative Colitis by Modulation of Monocyte Chemoattractant Protein 1 (MCP-1)/Nuclear Factor-Kappa B (NF-κB)/Interleukin-18 (IL-18)

“…Tissue sections were then collected through microtome sectioning, and finally, the slides were prepared and stained with hematoxylin and eosin (H&E) for light microscopy. Scoring of the histopathological findings was performed according to a four-point score depending on the assessment of mucosal necrosis and ulceration, submucosal oedema, hemorrhage, and inflammation in eight HPFs as described elsewhere [ 25 ]. For immunohistochemical staining, paraffin sections were adhered on coated slides, cleared in xylene, rehydrated, and then immersed in an antigen retrieval (EDTA solution, pH 8).…”

“…Subsequently, the slides counterstained with Mayer’s hematoxylin stain were covered with a glass cover slide. The labelling index of both NF-κB P65 and Caspase-3 were expressed as the percentage of cells (positive) per total 1000 counted cells using eight high-power fields [ 25 ].…”

Molecular and Cellular Mechanisms Influenced by Postbiotics

“…Postbiotics, both from CFS and HKB, mediate anti-inflammatory functions mostly via the attenuation of NF-κB activity [89][90][91][92]. The mechanisms of this attenuation frequently involve the inhibition of NF-κB nuclear translocation via the inhibition of IκB phosphorylation and the prevention of its proteasomal degradation [32,93,94]. Furthermore, the CFS of L. rhamnosus CNCM I-4036 was reported to enhance the expression of an IκB subunit, NFKBIA, and thus tighten NF-κB sequestering in the cytoplasm [95].…”

Probiotic dạng bào tử lợi khuẩn Bacillus đa chủng nồng độ cao hỗ trợ điều trị viêm ruột mạn tính

“…Chủng E. coli Nissle 1917 không gây bệnh 4 và Lactobacillus plantarum L.137 5 đã được chứng minh có hiệu quả như mesalamine, thúc đẩy sự bài tiết các cytokine chống viêm dùng điều trị bệnh Crohn. Hai chủng thuộc loài L. acidophilus được chứng minh có tác dụng hỗ trợ điều trị IBS.…”