Amelioration of rotenone‐induced alterations in energy/redox system, stress response and cytoskeleton proteins by octanoic acid in zebrafish: A proteomic study

Sürmen, Mustafa Gani; Sürmen, Saime; Cansız, Derya; Ünal, İsmail; Üstündağ, Ünsal Veli; Alturfan, A. Ata; Büyükkayhan, Derya; Emekli-Alturfan, Ebru

doi:10.1002/jbt.23024

Cited by 7 publications

(5 citation statements)

References 54 publications

(71 reference statements)

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“…This imbalance has also been identified as a key contributor to busulfan-induced reproductive toxicity [ 34 ]. Accumulating in vitro and in vivo evidence has elucidated the multifaceted mechanisms by which OCA ameliorates various diseases, notably through the alleviation of oxidative stress [ 20 , 35 ]. In this study, OCA treatment effectively improved busulfan-induced oxidative stress in the testes.…”

Section: Discussionmentioning

confidence: 99%

Octanoic acid mitigates busulfan-induced blood-testis barrier damage by alleviating oxidative stress and autophagy

Cao,

Zhang,

et al. 2024

Lipids Health Dis

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Background The management of male infertility continues to encounter an array of challenges and constraints, necessitating an in-depth exploration of novel therapeutic targets to enhance its efficacy. As an eight-carbon medium-chain fatty acid, octanoic acid (OCA) shows promise for improving health, yet its impact on spermatogenesis remains inadequately researched. Methods Mass spectrometry was performed to determine the fatty acid content and screen for a pivotal lipid component in the serum of patients with severe spermatogenesis disorders. The sperm quality was examined, and histopathological analysis and biotin tracer tests were performed to assess spermatogenesis function and the integrity of the blood-testis barrier (BTB) in vivo. Cell-based in vitro experiments were carried out to investigate the effects of OCA administration on Sertoli cell dysfunction. This research aimed to elucidate the mechanism by which OCA may influence the function of Sertoli cells. Results A pronounced reduction in OCA content was observed in the serum of patients with severe spermatogenesis disorders, indicating that OCA deficiency is related to spermatogenic disorders. The protective effect of OCA on reproduction was tested in a mouse model of spermatogenic disorder induced by busulfan at a dose 30 mg/kg body weight (BW). The mice in the study were separated into distinct groups and administered varying amounts of OCA, specifically at doses of 32, 64, 128, and 256 mg/kg BW. After evaluating sperm parameters, the most effective dose was determined to be 32 mg/kg BW. In vivo experiments showed that treatment with OCA significantly improved sperm quality, testicular histopathology and BTB integrity, which were damaged by busulfan. Moreover, OCA intervention reduced busulfan-induced oxidative stress and autophagy in mouse testes. In vitro, OCA pretreatment (100 µM) significantly ameliorated Sertoli cell dysfunction by alleviating busulfan (800 µM)-induced oxidative stress and autophagy. Moreover, rapamycin (5 µM)-induced autophagy led to Sertoli cell barrier dysfunction, while OCA administration exerted a protective effect by alleviating autophagy. Conclusions This study demonstrated that OCA administration suppressed oxidative stress and autophagy to alleviate busulfan-induced BTB damage. These findings provide a deeper understanding of the toxicology of busulfan and a promising avenue for the development of novel OCA-based therapies for male infertility.

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Section: Discussionmentioning

confidence: 99%

Octanoic acid mitigates busulfan-induced blood-testis barrier damage by alleviating oxidative stress and autophagy

Cao,

Zhang,

et al. 2024

Lipids Health Dis

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“…As the main fatty acid component of medium chain triglycerides in ketogenic diet, OA directly enters the liver through the portal vein, after being absorbed by the human body, bypassing the lymphatic system. NAD + /NADH could be improved by liver mitochondria to reduce metabolic dysfunction (26). Studies have shown that a diet rich in OA could increase the number of mitochondria and provide more energy for the body (27).…”

Section: Discussionmentioning

confidence: 99%

Octanoic Acid-rich Enteral Nutrition Alleviated Acute Liver Injury Through PPARγ/STAT-1/MyD88 Pathway in Endotoxemic Rats

Tang

et al. 2023

In Vivo

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Background/Aim: Acute liver injury is the hallmark of organ failure in sepsis. Enteral nutrition (EN) is an important clinical therapeutic measure in septic patients. However, the therapeutic effect of EN alone is not obvious. Here, we investigated whether octanoic acid (OA)-rich EN alleviated acute liver injury through PPARγ/STAT-1/MyD88 pathway in endotoxemic rats. Materials and Methods: First, rats were randomly divided into four groups: Sham, Lipopolysaccharide (LPS), LPS+EN and LPS+EN+OA groups to investigate the effect of OA-rich EN on LPSinduced acute liver injury in endotoxemic rats. Then rats were randomly divided into five groups: Sham, LPS, LPS+EN+OA, LPS+EN+OA+SR202 (SR) and LPS+ pioglitazone (PI) groups to examine whether OA-rich EN alleviated acute liver injury through the PPARγ/STAT-1/MyD88 pathway. Rats received nutrition support via a gastric tube for 3 days. We evaluated the liver histology, apoptosis, liver enzymes and inflammatory cytokine levels in the liver and serum. PPARγ/STAT-1/MyD88 pathway was also measured. Results: OA-rich EN inhibited the phosphorylation of STAT-1 and the activity of MyD88 by activating PPARγ and alleviating LPS-induced acute liver injury more effectively than EN alone in endotoxemic rats. The use of SR counteracted the effect of OA-rich EN on acute liver injury. Meanwhile, PI showed effects similar to OA-rich EN in endotoxemic rats. Conclusion: OA-rich EN alleviated acute liver injury through PPARγ/STAT-1/MyD88 pathway in endotoxemic rats.

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“…Then, the samples were centrifuged at 1000×g and the flow through were collected. The digestion protocol applied to obtain the tryptic peptide was similar to our previous study (8). An equal amount of protein for each sample was reduced with 20 mM DTT for 10 min at 95°C and then alkylated with 40 mM iodoacetamide for 20 min in the dark.…”

Section: Sample Preparationmentioning

confidence: 99%

“…The peptide mixtures were separated by a reverse phase nano-flow liquid chromatography (Dionex UltiMate 3000, Thermo Fisher Scientific) and identified using high resolution mass spectrometry (Q-Exactive Plus Orbitrap, Thermo Fisher Scientific) equipped with an electrospray ionization source. The analyses were performed by selecting the previous nano-UPLC-ESI-MS/MS system parameters (8). For analysis, each sample was re-dissolved in 0.1% formic acid and then loaded onto a trap column.…”

Section: Lc-ms/ms Analysis and Data Processingmentioning

confidence: 99%

Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach

Sürmen¹,

Bozkaya²,

Ateş³

et al. 2023

experimed

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Objective: Type 2 diabetes mellitus (T2D) is a metabolic disease whose molecular events have not yet been fully clarified. However, nextgeneration powerful molecular approaches such as mass spectrometry (MS)-based proteomics holds promise. In this study, we aimed to reveal the protein profile of serum samples obtained from patients with T2D and atherosclerotic cardiovascular disease using the highresolution liquid chromatography (LC)-MS/MS system. Materials and Methods:Immune depletion was performed for the top 12 abundant proteins in 10 µl serum samples taken from individuals. Then, tryptic peptides were obtained from total proteins by applying a digestion protocol. Accordingly, reduction, alkylation, and digestion with trypsin enzyme were carried out, respectively. Tryptic peptides were analyzed in an ultra-high-pressure LC-MS/MS system with a label-free proteomic approach. The raw data were processed using the software program.Results: LC-MS/MS analyses revealed 120 proteins with significant expression changes. Some of these proteins were associated with inflammation, lipid transport, and oxidative stress, which are known to play an important role in T2D and its complications. Conclusion:As a result, LC-MS/MS analyses highlighted the proteins that will provide predictions in the treatment and course of T2D. We believe that validation of these proteins with targeted proteomic approaches in a larger sample in further studies will contribute to the development of clinically usable panels.

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Amelioration of rotenone‐induced alterations in energy/redox system, stress response and cytoskeleton proteins by octanoic acid in zebrafish: A proteomic study

Cited by 7 publications

References 54 publications

Octanoic acid mitigates busulfan-induced blood-testis barrier damage by alleviating oxidative stress and autophagy

Octanoic acid mitigates busulfan-induced blood-testis barrier damage by alleviating oxidative stress and autophagy

Octanoic Acid-rich Enteral Nutrition Alleviated Acute Liver Injury Through PPARγ/STAT-1/MyD88 Pathway in Endotoxemic Rats

Large-Scale Proteomic Analysis of Patients with Type 2 Diabetes Mellitus and Atherosclerosis Using a Label-Free LC-MS/MS Approach

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