Amelioration of oxidative stress by Tabernamontana divaricataon alloxan-induced diabetic rats

Abstract

Objective:The purpose of this study was to evaluate the anti-diabetic activity of ethanol extract of Tabernamontana divaricata (L.) and its ameliorative effect on oxidative stress in alloxan-induced diabetic rats.Materials and Methods:Diabetes was induced by single intraperitoneal injection of alloxan monohydrate (140 mg/kg body weight). Methanol extract of T. divaricata was administered at the doses of 100 and 200 mg/kg body weight in diabetic induced rats including glibenclamide (3 mg/kg) as a reference drug. In the continuous 21 days treatment, fasting blood glucose level was determined on 0, 7, 14 and 21 days. On day 21, serum lipid profiles and glycosylated hemoglobin, liver antioxidant enzymes levels were estimated.Results:Experimental findings showed a significant anti-diabetic potential of the extract in terms of reduction in blood glucose levels and a correct effect on the altered biochemical parameters. Observed data were found statistically significant in correction of antioxidant enzyme level accompanied with diabetes, particularly at the dose of 200 mg/kg body weight.Conclusion:Based on the results, it can be concluded that the T. divaricata is found to be effective in type 2 diabetes in rats and to have an ameliorative effect on the associated oxidative stress.

Cryptolepine, an indoloquinoline alkaloid, in the management of diabetes mellitus and its associated complications

“…Healthy Sprague-Dawley rats weighing 170-200 g (fed for 14 h with 5% glucose solution to prevent hypoglycemic shock) were injected intraperitoneally with 140 mg/kg alloxan monohydrate in distilled water [ 11 ] after their fasting blood sugar (FBS) were ascertained by the enzymatic glucose oxidase method using a commercial OneTouch Select™ blood glucose meter (LifeScan Inc, Milpitas, CA, China). Drops of blood for the test were obtained from the tail vein of the rats.…”

<i>Tabernaemontana divaricata</i>: A Herbal Panacea

“…The results that came out showed a great amount of antidiabetic activity. Moreover, an additional decrease can also be seen in the oxidative damage effect that can be seen in rats 120 . Kanthlal et al suggest in their study that the insulin receptors may be alerted by an extract (methanolic).…”

“…Henceforth, it may result in the production of beta-stem cells in the subject of the test's pancreas. Antidiabetic activity can be seen by the compound conophylline, which is generally segregated from T. divaricata 120,121 . Increased plasma levels in diabetic rats were observed in the study.…”

Major Bioactive Alkaloids and Biological Activities of Tabernaemontana Species (Apocynaceae)

“…The antidiabetic activity of T. divaricata methanol extract was examined on alloxan-induced diabetic rats [ 251 ]. The results displayed substantial antidiabetic activity, with an additional reduction in the effect of oxidative damage observed in rats [ 251 ]. The extracts exhibited a similar mechanism in relation to the positive control, glibenclamide.…”

“…The extracts exhibited a similar mechanism in relation to the positive control, glibenclamide. The study of Kanthlal et al [ 251 ] recommends that the methanol extract may alert the insulin receptors, therefore triggering a stimulation or production of beta-stem cells in the pancreas of the test subjects. The compound conophylline, which is often isolated from serval Tabernaemontana species, displayed antidiabetic activity [ 252 ].…”