Amelioration of Ovalbumin-Induced Allergic Asthma by Juglans regia via Downregulation of Inflammatory Cytokines and Upregulation of Aquaporin-1 and Aquaporin-5 in Mice

Sharif, Mariam; Anjum, İrfan; Shabbir, Arham; Syed, Shahzada Khurram; Mobeen, Iqra; Shahid, Muhammad Hassaan; Sarwar, Kamran

doi:10.1155/2022/6530095

Cited by 5 publications

(4 citation statements)

References 31 publications

(30 reference statements)

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“…The current study also showed that treatment with EERc significantly suppressed the expression levels of TNF-α as compared with positive control or OVA-control. Similar results were obtained with methanol extract and its fractions (n-hexane and ethyl acetate) of Juglans regia kernels in mice [31]. The attenuation of allergic airway inflammation by EERc may be, in part, attributed to the suppression of TNF-α or may be responsible for the reduction of the number of leucocytes observed.…”

Section: Discussionsupporting

confidence: 82%

Effects of Rourea coccinea ethanolic root extract on ovalbumin-induced allergic asthma in rats

Dosseh¹,

Idoh²,

AGBODJOGBE³

et al. 2022

World J. Adv. Res. Rev.

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Rourea coccinea (syn. Byrsocarpus coccineus) Schum. and Thonn. (Connaraceae) is a medicinal plant that is being used for the treatment of various disorders in Tropical Africa. Previously, we reported anti-inflammatory and antioxidant effects of ethanolic extract of R. coccinea root bark (EERc). The present study aimed to test protective potential effects of EERc treatments against ovalbumin-induced airway lung inflammatory and to investigate some of its possible underlying mechanism on allergic asthma. Rats were sensitized and challenged with ovalbumin (OVA), and were orally treated daily with EERc at 200, 400 and 800 mg/kg from day 24 to day 27 post sensitization. The number of total and differential inflammatory cells, leakage of Evans blue dye tracer, histamine and TNF-α level in bronchoalveolar lavage (BAL) fluid were measured. OVA instillation induced airway lung inflammation, which was significantly suppressed by pre-treatment with EERc. Analysis of bronchoalveolar lavage (BAL) revealed that EERc pre-treatment significantly inhibited leukocytosis and reduced Evans blue dye content in the BAL fluid. EERc also significantly decreased the production and release of histamine and TNF-α. Our study shows that EERc possesses significant anti-allergic activity which might be attributed to reduction of histamine, TNF-α in the BAL fluid.

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Section: Discussionsupporting

confidence: 82%

Effects of Rourea coccinea ethanolic root extract on ovalbumin-induced allergic asthma in rats

Dosseh¹,

Idoh²,

AGBODJOGBE³

et al. 2022

World J. Adv. Res. Rev.

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“…After extraction, the RNA samples were processed to determine their quantity and purity using a nanodrop spectrophotometer [ 38 ]. Each sample containing 1000 ng RNA was reverse transcribed according to the kit manufacturer’s protocol (Thermo Scientific, Waltham, MA, USA) to synthesize cDNA [ 39 ]. Amplification of cDNA (1µL) was performed by mixing it with forward primer (1 µL), reverse primer (1 µL), nuclease-free water (2 µL), and PCR Master Mix (5 µL) (Thermo Scientific).…”

Section: Methodsmentioning

confidence: 99%

Anti-Arthritic and Immunomodulatory Potential of Methanolic, n-Hexane, and Ethyl Acetate Fractions of Bark of Acacia modesta on Complete Freund’s Adjuvant-Induced Arthritis in Rats

Mashaal,

Shabbir,

Khan

et al. 2023

Pharmaceutics

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Rheumatoid arthritis is an autoimmune disorder and topic of interest for researchers due to its increasing frequency and limited treatment. Acacia modesta Wall is known to treat rheumatic disorders in the traditional system of medicinal plants. Traditional medicines are still required for the treatment of this disease due to the large number of side-effects caused by commercial medicines. In the current study, the antiarthritic potential of methanolic extract (AM-metha), n-hexane (AM-hexa) fraction, and ethyl acetate (AM-etha) fraction of the bark of A. modesta against a complete Freund’s adjuvant rat model was evaluated. Evaluation using a digital plethysmometer, macroscopic evaluation, and histopathological evaluation were conducted to determine the paw volume and arthritic scoring. ELISA was performed to assess the PGE2 levels. RT-PCR was used to evaluate the expression levels of MMP2, MMP3, MMP9, NF-κB, IL6, IL1β, TNFα, and VEGF. Biochemical and hematological analyses were also conducted. GC/MS was also carried out to analyze the presence of medicinal compounds. The data revealed a marked reduction in the paw volume, arthritic scoring, and histopathological parameters, indicating the anti-arthritic potential of the plant. Treatment with plant extracts and fractions markedly down-regulated MMP2, MMP3, MMP9, NF-κB, IL6, IL1β, TNFα, and VEGF levels. Similarly, PGE2 levels were also found to be ameliorated in the treatment groups, indicating the immunomodulatory property of plant bark. Plant treatment nearly normalized hematological parameters such as counts of WBCs, RBCs, and platelets, along with Hb content, thereby validating the anti-arthritic activity. GC/MS analysis disclosed the presence of strong anti-inflammatory compounds such as lupeol, oleic acid, and squalene. The study showed that A. modesta possesses anti-arthritic and immunomodulatory potential linked to significant down-regulation of pro-inflammatory and inflammatory biomarkers.

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“…63 Juglans regia was shown to improve allergic asthma by inhibiting inflammatory cytokines and elevating protein expression levels of AQP-4, and AQP-5. 64 Another study stated that AQP3 potentiated ovalbumin-induced mouse asthma by facilitating H 2 O 2 membrane permeability, increasing chemokine production (CCL22 and CCL24), and T cell trafficking from alveolar macrophages. 65 …”

Section: Introductionmentioning

confidence: 99%

Aquaporins: Potential Targets in Inflammatory Diseases

Bozkurt,

Halici,

Yayla

2024

The Eurasian Journal of Medicine

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Inflammation involves a long chain of molecular reactions and cellular activity designed to repair tissue damaged by various causes. The inflammatory process and its complex mechanisms have recently become a focus of interest for many researchers. After the onset of inflammation, various adverse conditions that initiate the inflammatory response need to be addressed; however, failure to limit the inflammatory reaction may result in the damage or destruction of host cells. Therefore, inflammatory reactions play a role in many different diseases. Aquaporins (AQPs), commonly referred to as water channels, are protein channels responsible for forming pores in the membranes of biological cells. Their main function is to aid in the movement of water between cells. Aquaporins not only regulate transepithelial fluid transport across membranes but also play a role in regulating essential events crucial for the inflammatory response. Aquaporins have been shown in many studies to have important roles in inflammatory diseases. This clearly indicates that AQPs may be potential targets for inflammatory diseases. This review summarizes the research to date on the structure and function of AQPs and provides an update on the relationship between AQPs and various human inflammatory diseases.

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Amelioration of Ovalbumin-Induced Allergic Asthma by Juglans regia via Downregulation of Inflammatory Cytokines and Upregulation of Aquaporin-1 and Aquaporin-5 in Mice

Cited by 5 publications

References 31 publications

Effects of Rourea coccinea ethanolic root extract on ovalbumin-induced allergic asthma in rats

Effects of Rourea coccinea ethanolic root extract on ovalbumin-induced allergic asthma in rats

Anti-Arthritic and Immunomodulatory Potential of Methanolic, n-Hexane, and Ethyl Acetate Fractions of Bark of Acacia modesta on Complete Freund’s Adjuvant-Induced Arthritis in Rats

Aquaporins: Potential Targets in Inflammatory Diseases

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