Amelioration of Muc5b mucin hypersecretion is enhanced by IL-33 after 2-APB administration in a murine model of allergic rhinitis

Lin, Lili; Zhao, Xia; Yan, Weili; Guo, Yongwei; Liang, Saisai

doi:10.3109/10520295.2013.839827

Cited by 9 publications

(8 citation statements)

References 33 publications

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“…In healthy individuals, MUC5B is mainly expressed in submucosal glands whereas MUC5AC is exclusively present in goblet cells. Lin et al [13] showed that 2-aminoethoxydiphenyl borate (2-APB), a chemical that potentially inhibits intracellular calcium release through modification of TRP channels, reduced MUC5B secretion from submucosal glands whereas IL-33 enhanced its secretion into the nasal mucus of allergic rhinitis mouse models. 2-APB also reduced IL-4, IL-5, and IL-13 in nasal mucus as well as in the epithelium.…”

Section: Mucinsmentioning

confidence: 99%

Nasal mucus proteome and its involvement in allergic rhinitis

Tomazic

Darnhofer

Birner‐Gruenberger

2020

Expert Review of Proteomics

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Introduction: Nasal mucus is the first line defense barrier against various pathogens including allergens. Proteins in nasal mucus maybe used as biomarkers for diagnosis or future therapeutic strategies. Proteomics opens the possibility to investigate whole human proteomes. Areas Covered: We aimed to analyze the existing literature on nasal mucus and nasal secretions proteomic approaches especially in allergic rhinitis. A PubMed/Medline search was conducted entering the following keywords and combinations: "nasal mucus", "nasal lavage fluid," nasal secretions," "nasal swabs," "allergic rhinitis," "proteins," and "proteomics." Expert opinion: The majority of studies focus on single proteins or protein groups mainly using ELISA techniques. Four studies met the criteria using mass spectrometry in the analysis of nasal mucus proteomes in rhinologic diseases. In these studies, 7, 35, 267, and 430 proteins were identified, respectively. These four studies are discussed in this review and put in relation to seven other proteomic studies that focus on nasal lavage fluid and nasal secretions obtained by swabs or filter paper. To put it in a nutshell, proteomics facilitates the investigation of the nasal secretome and its role in healthy and diseased state and as potential biomarkers for new diagnostic or therapeutic approaches. ARTICLE HISTORY

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Section: Mucinsmentioning

confidence: 99%

Nasal mucus proteome and its involvement in allergic rhinitis

Tomazic

Darnhofer

Birner‐Gruenberger

2020

Expert Review of Proteomics

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“…29,30 These processes are pivotal events in the mechanisms of AR. Therefore, based on previous studies, [31][32][33][34][35][36] we hypothesized that there might be expression of Orai1 in nuocytes, and the intervention of this protein might work in the attenuation of allergic reactions.…”

Section: Discussionmentioning

confidence: 99%

Intervention of Orai1 Influences the Response of Nuocytes From Allergic Rhinitis Mice to IL-33

Lin

Wei

Chen

et al. 2019

Ann Otol Rhinol Laryngol

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Objective: Nuocytes are essential in innate type-2 immunity and contribute to the exacerbation of allergic rhinitis (AR). This study aimed to evaluate the intervention of Orai1 on the response of nuocytes from AR mice to interleukin (IL)-33. Methods: We established a murine model of AR. Nuocytes were obtained from the mouse nasal-associated lymphoid tissue. Then, we assessed expressions of Orai1, Ca2+ mean fluorescence intensity (MFI) in nuocytes, and their cellular response to mouse recombinant (rm) IL-33. After that, we administered rmlentivirus vectors (lenti) that encoded small hairpin RNA (shRNA) against ORAI1 (lenti-ORAI1) into nuocytes cultures and again evaluated Orai1 and Ca2+ MFI in nuocytes and their response to rmIL-33. Finally, we adoptively transferred nuocytes alone or nuocytes transfected by lenti or lenti-ORAI1 to AR models to investigate their roles during allergic inflammation. Results: We showed that Orai1 and Ca2+ MFI were upregulated in AR mice nuocytes. These cells were induced to produce more IL-5 and IL-13 by rmIL-33. However, the intervention of Orai1 by lenti-ORAI1 in nuocytes decreased Orai1 and Ca2+ MFI and reduced productions of aforementioned cytokines even after the administration of rmIL-33. Numbers of sneezing, nasal rubbing, and counts of eosinophils were all enhanced after the adoptive transfer of nuocytes. Concentrations of IL-5, IL-13, and IL-33 in the nasal lavage fluid (NLF) of allergic mice were also increased. However, the adoptive transfer of nuocytes transfected by lenti-ORAI1 decreased aforementioned parameters. Conclusion: These findings show that the intervention of Orai1 in nuocytes influences the response of nuocytes to rmIL-33.

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“…4c, j). Goblet cells and submucosal glands were reported to play a role in the development of CRS [20] and allergic rhinitis [21,22]. Therefore, we made experiments to determine these 2 types of cells before and after the treatment with budesonide using HE or PAS staining.…”

Section: Histological Analysesmentioning

confidence: 99%

Efficacy of Budesonide Nasal Spray on Neutrophilic Chronic Rhinosinusitis with Nasal Polyps: A Combined Clinical and Experimental Study

Lin

Lan

Dai

et al. 2020

Int Arch Allergy Immunol

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Background: Neutrophilic chronic rhinosinusitis with nasal polyps (CRSwNP) occur predominantly in Asian subjects. Appropriate treatments for this endotype have not been elucidated. This study aimed to evaluate the efficacy of budesonide nasal spray on neutrophilic CRSwNP. Materials and Methods: Fifteen neutrophilic CRSwNP patients were included, and then they received budesonide nasal spray treatment for 3 months. Biopsies of nasal polyps (NPs) were obtained from these subjects. Their clinical indexes were scored using Visual Analog Scale (VAS), Sino-Nasal Outcome Test (SNOT)-22, and Endoscopic Appearances (EAs). Histological analyses were used to assess numbers of neutrophils, goblet cells, and submucosal gland cells in NPs. Percentages of CD8⁺ T cells and CD4⁺CD25⁺Foxp3⁺ regulatory T cells (Tregs) were evaluated using flow cytometry. Mucin 5AC (MUC5AC), MUC5B, myeloperoxidase (MPO), interferon (IFN)-γ, and interleukin (IL)-1β and their mRNAs were also examined. After that, we cultured NP tissues in vitro and evaluated the abovementioned inflammatory parameters before and after the administration of budesonide. Results: Budesonide nasal spray did not improve clinical evaluations including VAS, SNOT-22, and EA scores. Numbers of neutrophils and goblet cells, the score of submucosal gland cells, percentages of CD8⁺ T cells and Tregs, MUC5AC, MUC5B, MPO, IFN-γ, and IL-1β and their mRNAs were not decreased in NPs after the budesonide treatment. Furthermore, the administration of budesonide into NP cultures also did not reduce their levels in comparison with those before the treatment. Conclusion: These findings demonstrate that budesonide treatment may not alleviate the inflammatory condition in neutrophilic CRSwNP.

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Amelioration of Muc5b mucin hypersecretion is enhanced by IL-33 after 2-APB administration in a murine model of allergic rhinitis

Cited by 9 publications

References 33 publications

Nasal mucus proteome and its involvement in allergic rhinitis

Nasal mucus proteome and its involvement in allergic rhinitis

Intervention of Orai1 Influences the Response of Nuocytes From Allergic Rhinitis Mice to IL-33

Efficacy of Budesonide Nasal Spray on Neutrophilic Chronic Rhinosinusitis with Nasal Polyps: A Combined Clinical and Experimental Study

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