Amelioration of lipid accumulations and metabolism disorders in differentiation and development of 3T3-L1 adipocytes through mulberry leaf water extract

Du, Yan; Li, Ding-Xiang; Lu, Dong-yu; Zhang, Ran; Zhong, Qiao-Qiao; Zhao, Yan-Lin; Zheng, Xiao-xiao; Ji, Shuai; Wang, Liang; Tang, Daoquan

doi:10.1016/j.phymed.2022.153959

Cited by 16 publications

(6 citation statements)

References 47 publications

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“…Leptin and adiponectin play regulatory roles in glycolipid metabolism, and their deficiency leads to increased free fatty acids and accumulation of hepatic lipids. Then, we explored the effect of MLE at a deeper level on lipid accumulation in AML-12 cells, and the results showed that RNA levels of leptin and adiponectin were increased, which was consistent with the trend reported in the literature [35,36]. Changes in the expression levels of lipid metabolism-related proteins in cells showed that the effect of MLE went deep into the protein level.…”

Section: Discussionsupporting

confidence: 85%

Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo

Xing

Ren

et al. 2022

Molecules

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Metabolic syndrome (MS) is a metabolic disease with multiple complications. Mulberry leaf extract (MLE) is rich in flavonoids and has great potential in alleviating glucose and lipid metabolism disorders. This study evaluated the effect and mechanism of MLE on the alleviation of MS. The components of the MLE were analyzed, and then the regulation of lipid metabolism by MLE in vitro and in vivo was determined. In a hepatocyte model of oleic acid-induced lipid accumulation, it was found that MLE alleviated lipid accumulation and decreased the expression of genes involved in lipogenesis. Furthermore, MLE improved obesity, insulin resistance, plasma lipid profile, and liver function in MS mice after a 15-week intervention. MLE decreased the expression of SREBP1, ACC, and FAS through the AMPK signaling pathway to inhibit lipid synthesis and increase the level of CPT1A to promote lipid decomposition to achieve its hypolipidemic effect. Meanwhile, MLE was also shown to affect the composition of the gut microbiota and the production of short-chain fatty acids, which contributed to the alleviation of lipid accumulation. Our results suggest that MLE can improve MS by improving lipid metabolism through multiple mechanisms and can be developed into dietary supplements for the improvement of MS.

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Section: Discussionsupporting

confidence: 85%

Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo

Xing

Ren

et al. 2022

Molecules

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“…The cells were washed with 60% isopropanol/water 3-fold to remove the excess stain. Cell-accumulated Oil Red O dye was extracted using 100% isopropanol, and absorbance was measured at 500 nm by a microplate reader [35,36]. Caffeine and adrenaline were used as positive controls.…”

Section: Quantification Of Cellular Lipid Contentsmentioning

confidence: 99%

A Thai Traditional Triple-Fruit Formulation “Phikud Tri-Phon” May Provide Fat Loss and Nutritional Benefits

Ngamdokmai

Ingkaninan

Scholfield

et al. 2022

Foods

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Obesity and overweight have serious health outcomes. “Phikud Tri-Phon” (PTP) is a traditional Thai medicine comprising three dried fruits from Aegle marmelos L., Morinda citrifolia L., and Coriandrum sativum L. Whether this medicine impacts on metabolic disease is unclear. This study aimed to investigate the phenolic and flavonoid contents of PTP and each of its herbal components, and further assess their antioxidant and anti-adipogenetic activities. Oil-red O staining was measured for lipid accumulation in 3T3-L1 adipocytes. The chemical profiles of PTP and each herbal extract were determined by LC-ESI-QTOF-MS/MS. Our results show that the total phenolic and flavonoid contents of PTP water extract were 22.35–108.42 mg of gallic acid equivalents and PTP ethanolic extract was 1.19–0.93 mg of quercetin equivalents and the DPPH scavenging capacity assay of PTP ethanolic extract (1 mg/mL) was 92.45 ± 6.58 (Trolox equivalent)/g. The PTP extracts and individual herbs had inhibitory adipogenesis activity, which reduced lipid accumulation by approximately 31% in PTP water extract and 22% in PTP ethanolic extract compared with control cells. These results provided insights into the traditional preparation method of using boiling water as a vehicle for PTP. In conclusion, PTP has antioxidant and anti-adipogenesis potential, indicating it is a promising ingredient in functional food and herbal health products.

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“…Mulberry leaf tea has been recorded for diabetes treatment in “ Compendium of Materia Medica ,” which was booked by the pharmacist Shi‐Zhen Li of the Ming Dynasty in ancient China. Recently, our group has demonstrated that mulberry leaf water or ethanol extracts can improve lipid metabolism disorder, glucose homeostasis, inflammation, oxidative stress, insulin secretion, and gut microbiota imbalance in high‐fat diet and/or streptozotocin (STZ)‐induced obese or type 2 diabetic mice (Du, Li, Lu, Zhang, Zhao, et al, 2022; Du, Li, Lu, Zhang, Zheng, 2002; Du, Li, Lu, Zhang, Zhong, et al, 2022; Ji et al, 2021). However, studies about the impact of mulberry leaf on BCAAs metabolism and relative gut microbiota in T2DM are still limited.…”

Section: Introductionmentioning

confidence: 99%

Mulberry leaf water extract alleviates type 2 diabetes in mice via modulating gut microbiota‐host co‐metabolism of branched‐chain amino acid

Zheng

et al. 2023

Phytotherapy Research

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Elevations in circling branched‐chain amino acids (BCAAs) levels associated with insulin resistance and type 2 diabetes mellitus (T2DM). Morus alba L. water extracts (MLE) show hypoglycemic function, but the precise mechanism remains obscure. This study is designed to investigate the association of the antidiabetes effect of MLE with the BCAAs co‐metabolism modulated by host and gut microbiota. Tissue‐specific expressions of BCAA‐catabolizing enzymes were detected by RT‐PCR and western blot, respectively. The components of the intestinal microflora were analyzed by high‐throughput 16S rRNA gene sequencing. The results showed that MLE administration improved blood glucose and insulin level, decreased inflammatory cytokines expression, and lowered serum and feces BCAAs levels. Furthermore, MLE reversed the abundance changes of the bacterial genera correlated with serum and feces BCAAs, such as Anaerovorax, Bilophila, Blautia, Colidextribacter, Dubosiella, Intestinimonas, Lachnoclostridium, Lachnospiraceae_NK4A136, Oscillibacter, and Roseburia. Functionality prediction indicated that MLE potentially inhibited bacterial BCAAs biosynthesis, and promoted the tissue‐specific expression of BCAAs catabolic enzyme. More importantly, MLE had obvious impacts on BCAA catabolism in germ‐free‐mimic T2DM mice. Those results indicated that MLE improving T2DM‐related biochemical abnormalities is associated with not only gut microbiota modification but also the tissue‐specific expression of BCAAs catabolic enzyme.

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Amelioration of lipid accumulations and metabolism disorders in differentiation and development of 3T3-L1 adipocytes through mulberry leaf water extract

Cited by 16 publications

References 47 publications

Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo

Mulberry Leaf Extract Improves Metabolic Syndrome by Alleviating Lipid Accumulation In Vitro and In Vivo

A Thai Traditional Triple-Fruit Formulation “Phikud Tri-Phon” May Provide Fat Loss and Nutritional Benefits

Mulberry leaf water extract alleviates type 2 diabetes in mice via modulating gut microbiota‐host co‐metabolism of branched‐chain amino acid

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