Amelioration of ischemia-reperfusion induced functional and biochemical deficit in mice by Ocimum kilimandscharicum leaf extract

Singh, Varinder; Krishan, Pawan; Singh, Nirmal; Kumar, Amit; Shri, Richa

doi:10.1016/j.biopha.2016.11.064

Cited by 19 publications

(20 citation statements)

References 57 publications

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“…These effects are mainly associated with their composition in antioxidant compounds such as flavonoids and terpenoids. Plants of the family Lamiaceae, Annonaceae, and Geraniaceae have been traditionally used to treat central nervous system pathological conditions in several models, including cerebral ischemia [11][12][13]. Therefore, we include plants of these families in our study.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Properties and Protective Effects of Some Species of the Annonaceae, Lamiaceae, and Geraniaceae Families against Neuronal Damage Induced by Excitotoxicity and Cerebral Ischemia

et al. 2020

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This study aimed to compare the antioxidant activities of extracts obtained from three plant families and evaluate their therapeutic effect on strokes. Ethanol extracts were obtained from either the leaf or the aerial parts of plants of the families Annonaceae (Annona cherimola, A. diversifolia, A. muricata, A. purpurea, and A. reticulata), Lamiaceae (Salvia amaríssima and S. polystachya), and Geraniaceae (Geranium niveum and G. mexicanum). Extracts were analyzed in terms of hydroxyl radical (OH•), peroxyl radical (ROO•), and superoxide anion (O2•−). The efficiency of the extracts to prevent neuronal death induced by excitotoxicity was tested with the tetrazolium assay, the O2•− scavenging capacity was evaluated with the dihydroethidium dye, and the protective effect of the extracts with the highest antioxidant activity was tested on a stroke experimental model. The extracts’ IC50 values (μg/mL) of scavenging varied from 98.9 to 155.04, 4.5 to 102.4, and 20.2 to 118.97 for OH•, ROO•, and O2•−, respectively. In the excitotoxicity model, Annonaceae extracts were highly cytotoxic while Lamiaceae and Geraniaceae reduced intracellular O2•− production and protect neurons against oxidative stress. Salvia polystachya reduced cerebral damage, as well as improved survival and behavior after ischemia. Our results encouraged the use of plant extracts as natural antioxidants to minimize neuronal injury following stroke.

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Section: Introductionmentioning

confidence: 99%

Antioxidant Properties and Protective Effects of Some Species of the Annonaceae, Lamiaceae, and Geraniaceae Families against Neuronal Damage Induced by Excitotoxicity and Cerebral Ischemia

et al. 2020

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“…The bilateral common carotid artery occlusion (BCCAO) method as explained by Singh, Krishan, Singh, Kumar, and Shri (2017) was employed for induction of cerebral I‐R injury in mice. In brief, animals were anaesthetized with chloral hydrate (350 mg/kg, i.p.)…”

Section: Methodsmentioning

confidence: 99%

“…The Morris water maze (MWM) test was performed, as described in previous reports, to evaluate the learning ability and memory functions of mice (Morris, 1984; Singh et al., 2017). Briefly, MWM was consisted of a large circular water pool (height 45 cm and diameter 150 cm, filled to a depth of 30 cm).…”

Section: Methodsmentioning

confidence: 99%

“…The neurological severity score (NSS), ranging from 0 to 15, was employed to evaluate the effect of various treatments on neurological functions of animals. NSS measures balance, motor, and reflex parameters of animals in a composite manner as described in Table 1 (Singh et al., 2017). A greater NSS signifies higher neurological dysfunction.…”

Section: Methodsmentioning

confidence: 99%

“…The brain samples were cut coronally (1 mm thick) and stained with 2,3,5‐triphenyltetrazolium chloride (TTC) as outlined by Singh et al. (2017). The stained sections were digitalized, and yellow area (infarcted area) was calculated with Image J software (NIH Image 1.61; National Institute of Health, Bethesda, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

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Isolation and characterization of components responsible for neuroprotective effects of Allium cepa outer scale extract against ischemia reperfusion induced cerebral injury in mice

Singh

Shri

Krishan

et al. 2020

Journal of Food Science

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The antioxidant‐mediated neuroprotective effect of Allium cepa outer scale extract (ACE) in mice with cerebral ischemia‐reperfusion (I‐R) injury was demonstrated in our earlier work. The current investigation aimed at establishing the bioactive component(s) responsible for this activity. Thus ACE was fractionated into ethyl acetate (EF) and aqueous (AF) fractions. These fractions were evaluated against cerebral I‐R injury in mice. I‐R injury in mice was induced by bilateral common carotid artery occlusion followed by 24 hr reperfusion. Memory, sensorimotor functions, cerebral infarct size, and oxidative stress were measured to address the neuroprotective mechanism of test substances. EF showed marked improvement of memory and sensorimotor functions by reducing brain oxidative stress and infarct size in mice after I‐R injury. The bioactive EF was subjected to chromatographic (HPLC‐PDA, HPLC‐MS, preparative HPLC) and spectroscopic studies to isolate and identify the neuroprotective compounds. This lead to separation of three components, namely quercetin, quercetin 4′‐O‐glucoside, and the remaining fraction, from EF. The separated components were biologically evaluated. These components showed improvement in mice with I‐R injury. But, EF displayed more marked neuroprotective effects as compared to the isolated components. The distinct neuroprotective outcome of EF may be credited to the synergistic action of compounds present in EF. Further studies such as evaluation of neurotoxic effects and other possible neuroprotective mechanisms are required to develop EF as a neuroprotective drug.

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A Comprehensive Review of the Pharmacological Activities of Ocimum kilimandscharicum: Camphor Basil

Chincholikar,

Malviya,

Saxena

et al. 2024

Macromolecular Symposia

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Ocimum kilimandscharicum or Camphor basil is a plant that is native to the Kikuyu tribes of Kenya. This plant has been widely known for its mosquito repellant properties but its potential as a therapeutic agent remains a lesser‐known fact. Traditional/Folkloric uses of this plant have paved way for the numerous studies conducted till now and these have helped to establish this plant as one of medicinal value. The well‐known folkloric uses of this plant include treating flu, cancer, diarrhea, asthma, ulcer, measles, cough, and abdominal pain among others. These studies provide insights into the efficacy of this plant in comparison to other species belonging to the same genus. This review aims to emphasize the various therapeutic and nontherapeutic applications of O. kilimandscharicum with a brief outline of its phytochemical constituents.

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Amelioration of ischemia-reperfusion induced functional and biochemical deficit in mice by Ocimum kilimandscharicum leaf extract

Cited by 19 publications

References 57 publications

Antioxidant Properties and Protective Effects of Some Species of the Annonaceae, Lamiaceae, and Geraniaceae Families against Neuronal Damage Induced by Excitotoxicity and Cerebral Ischemia

Antioxidant Properties and Protective Effects of Some Species of the Annonaceae, Lamiaceae, and Geraniaceae Families against Neuronal Damage Induced by Excitotoxicity and Cerebral Ischemia

Isolation and characterization of components responsible for neuroprotective effects of Allium cepa outer scale extract against ischemia reperfusion induced cerebral injury in mice

A Comprehensive Review of the Pharmacological Activities of Ocimum kilimandscharicum: Camphor Basil

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