Amelioration of<i>Escherichia coli</i>‐induced endometritis with ascorbic acid in non‐pregnant mouse models

Bafor, Enitome E.; Onwukpa, Ikechukwu; Itemire, Anne Oghenekevwe; Omoruyi, Osemelomen; Eferoba-Idio, Ejiroghene; Odega, Kevin; Eghianruwa, Osas Jim

doi:10.1111/aji.12976

Cited by 5 publications

(3 citation statements)

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“…In rats, the uterus consists of a columnar luminal epithelium surrounded by stromal cells, and endometrial glands in the normal endometrium are mainly present in the endometrial region of the uterus . This phenomenon was observed in the blank control group (endometritis‐free), the model group, as well as in the nisin and kanamycin groups (Figure ).…”

Section: Resultsmentioning

confidence: 80%

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Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

Jia

Wang

et al. 2019

American J Rep Immunol

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Problem The Staphylococcus aureus has been found to be associated with clinical endometritis of cow. The result of oral antibiotic remains poor. Therefore, this study investigates the role of nisin in endometritis. Method of study The effect of nisin on the growth and cell wall of S aureus were determined in vitro. Besides the blank control group, animals with established post‐partum were inoculated with 0.1 mL S aureus intravaginally. Two days post‐inoculation, the animals were administered nisin (25 mg/kg), kanamycin (30 mg/kg), and water (model group) for 7 days. On the seventh day, serum and uterine organs were obtained for pro‐ and anti‐inflammatory analysis. The uterine tissue samples were weighed, and histopathological analysis was performed. Results The results showed that nisin had an inhibitory effect on the growth and cell wall formation of S aureus. Nisin and kanamycin treatment prevented a S aureus‐induced decrease in pro‐inflammatory cytokines and promoted an increase in the level of serum anti‐inflammatory cytokines in the endometrium of these animals. Nisin and kanamycin, significantly decreased (P < 0.05) the endometritis‐induced increases in uterine weight, restored endometrial architecture and significantly (P < 0.05) normalized uterine neutrophils to control levels. Additionally, improved levels of B7‐2, IFN‐γ, IL‐2, and IL‐8 were observed when treated with nisin. Conclusion Our findings suggest that nisin compared favorably with kanamycin in endometritis prevention, suggesting that nisin can be used in S aureus‐induced endometritis by protecting the uterus from S aureus infection.

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Section: Resultsmentioning

confidence: 80%

“…A wide range of microbial infections of the uterine cavity are able to cause endometritis . Infection of the endometrium by S aureus often leads to severe uterine diseases in humans and animals, and the endometrium as the first line of defense against pathogen invaders provides an innate immune function .…”

Section: Introductionmentioning

confidence: 99%

Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

Jia

Wang

et al. 2019

American J Rep Immunol

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“…AA (Marin Liza Pharmacy, Indonesia) was given 1000 mg/kg body weight/day for 5 days 36 h after E. coli inoculation (based on previous research for diffuse acute endometritis) [5], [16], [17]. AA tablets were crushed, dissolved in aquades, and given orally, with a ten cc syringe.…”

Section: Administration Of Aamentioning

confidence: 99%

The Effect of Ascorbic Acid on Interleukin-10 and Tumor Necrosis Factor-α Cytokines in Rattus norvegicus with Endometritis

Prabudi

Siregar

Nasution

et al. 2021

Open Access Maced J Med Sci

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BACKGROUND: Endometritis is a gynecological disease characterized by inflammation of the endometrial glands and stroma. Inflammatory stimuli or tissue injury induce inflammatory pain through the release of cytokines. Ascorbic acid (AA) is a water-soluble Vitamin that plays a role in inhibiting the production of proinflammatory cytokines and increases the expression of anti-inflammatory cytokines. AIM: The purpose of this study was to find out the association between administration of AA and inflammatory cytokines in experimental animals Rattus norvegicus with endometritis. METHODS: The research was conducted using virgin female R. norvegicus laboratory mice weighing 250–300 g and aged 11–12 weeks with an estrus cycle of 5–6 days. Mice with regular oestrous cycles were randomly divided into three groups: group 1 was given 200 L of water orally without Escherichia coli inoculation and represented a negative control. Groups 2 and 3 were inoculated (50 L/rat) E. coli intravaginally, 106 colony-forming unit/mL, Group 2 was not given AA and the other side Group 3 was assigned AA. The interleukin (IL)-10 and tumor necrosis factor (TNF)-α _cytokines examination was carried out by histopathological examination through a biopsy of the endometrial tissue. Hypothesis testing on the data was analyzed by the Kruskal Wallis test using Statistical Package for Social Sciences. RESULTS: Data from the current study revealed that the highest mean value of IL-10 was found in the negative control group (2.5) and the lowest value in the positive control group (1.3). Regarding TNF-α _the highest mean value (2.8) was found in the treatment group and the lowest mean value (2.1) was found in the treatment group. Using the Kruskal Wallis test, IL-10 and TNF-α _showed insignificant results (p = value 0.304 and 0.145 respectively). CONCLUSIONS: The administration of AA did not affect the decrease in TNF-α _or the upregulation of IL-10 as anti-inflammatory cytokines.

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Ascorbic Acid and Alpha-Tocopherol Contribute to the Therapy of Polycystic Ovarian Syndrome in Mouse Models

et al. 2020

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Amelioration ofEscherichia coli‐induced endometritis with ascorbic acid in non‐pregnant mouse models

Abstract: AA compared favourably with amoxicillin in endometritis management, suggesting that AA can be used in the management of infection-induced endometritis, normal cycling and normal uterine function.

Cited by 5 publications

References 43 publications

Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

The Effect of Ascorbic Acid on Interleukin-10 and Tumor Necrosis Factor-α Cytokines in Rattus norvegicus with Endometritis

Ascorbic Acid and Alpha-Tocopherol Contribute to the Therapy of Polycystic Ovarian Syndrome in Mouse Models

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