Amelioration of Hyperglycaemia, Oxidative Stress and Dyslipidaemia in Alloxan-Induced Diabetic Wistar Rats Treated with Probiotic and Vitamin C

Abstract

Clinical and experimental evidence suggests that hyperglycaemia is responsible for the oxidative stress in diabetes mellitus. The study was designed to investigate the comparative effects of probiotic and vitamin C (Vit-C) treatments on hyperglycaemia, oxidative stress and dyslipidaemia in alloxan-induced diabetic rats. Type 1 diabetes (T1DM) was induced in male Wistar rats by a single intraperitoneal (i.p.) injection of alloxan (150 mg/kg). Six groups of the animals received the following treatment regimens for four weeks: (1) Normal saline, per os; (2) alloxan (150 mg/kg, i.p.); (3) alloxan (150 mg/kg) + insulin (4 U/kg, subcutaneously); (4) alloxan (150 mg/kg) + probiotic (4.125 × 106 CFU/100 mL per os); (5) alloxan (150 mg/kg) + Vit-C (100 mg/kg, i.m.); (6) alloxan (150 mg/kg) + probiotic (4.125 × 106 CFU/100 mL per os) + Vit-C (100 mg/kg, intramuscularly). Probiotic + Vit-C decreased (p < 0.05) blood glucose concentration in diabetic treated group, when compared with the untreated diabetic group. Probiotic + Vit-C reduced malondialdehyde concentration, in the serum, brain and kidneys, respectively, but increased the activity of antioxidant enzymes. Probiotic and Vit-C may be more effective than Vit-C alone, in ameliorating hyperglycaemia, oxidative stress and dyslipidaemia in alloxan-induced diabetic rats.

Protective effects of Cynara scolymus leaves extract on metabolic disorders and oxidative stress in alloxan-diabetic rats

“…The development of oxidative stress (OS), underlies the major complications in DM patients [5], OS is an imbalance between oxidants and antioxidants in favor of the former, potentially leading to cell damage and destruction [6]. Several experimental studies have suggested that the increased generation of reactive oxygen species (ROS) causes damage to cells, tissues like pancreas, kidney and liver contributed to diabetic complications [7].…”

The Role of Oxidative Stress and Antioxidants in Diabetes Mellitus

“…diyabetik ratlardan bir gruba A, C ve E vitaminleri diğer gruba da ω-3 yağ asiti verdiklerinde 4 hafta sonunda kontrol diyabetiklerde kalpteki SOD enziminin yanısıra kalp ve karaciğerdeki CAT enzimlerinin anlamlı olarak azaldığını (p<0.001) buna karşılık A, C ve E vitamini verilenlerde kalp CAT enziminin anlamlı olarak artığını (p=0.05) ve ω-3 yağ asiti verilen diyabetik ratlarda ise enzim düzeylerinde herhangi bir değişiklik olmadığını tespit etmişlerdir (51). Bir başka çalışmada alloksan ile Tip 1 diyabet yapılmış ratlarda C vitaminiyle birlikte probiyotik verilmesinin hem glukoz düzeyini hem de oksidatif stresi düşürdüğü buna karşılık antioksidan düzeyini artırdığı görülmüştür (52). Diyabetik ratlara 200mg/kg dozda E vitamini takviyesi yapıldığında plazma glukoz düzeyinin azalmadığı ancak MDA düzeyinin azaldığı ve antioksidan enzimlerle (CAT, GSH-PX, GSH-RD) antioksidan (E vitamini, C vitamini, ürik asit, eritrosit glutatyon düzeylerinin) normal seviyede olduğu tespit edilmiştir (53).…”

Effect of Probiotic Supplementation on Blood Pressure of Females with Gestational Diabetes Mellitus: A Randomized Double Blind Controlled Clinical Trial

“…Microbes in the gut contribute to extraction of energy from food and should be considered an environmental factor involved in obesity and related disabilities, such as insulin resistance, diabetes, and cardiovascular disease (28)(29)(30).…”