Amelioration of experimental nephrotoxicity due to 5-Flourouracil by Resveratrol in comparison to Vitamin-E

Abstract

The therapeutic efficacy of Resveratrol (RSV) and Vitamin E were studied against 5-Flourouracil (5-FU) induced nephrotoxicity. 36 male Wistar rats were selected randomly weighing between 150-180 g and are made into 6 groups, each group containing 6 rats. Group 1 was maintained as sham. 5-Flourouracil was administered to groups 2, 5 and 6 intraperitoneally (20 mg/kg body weight) on day 1, 3 and 7. Group 2 was kept as positive control (administered 5-FU intraperitoneally). Groups 3, 5 and 4, 6 were administered vitamin E and resveratrol per orally for 14 days @ 200 mg/kg bwt. At the end of the experiment the blood was withdrawn and serum analyzed for renal biomarkers. For histopathological studies, samples of kidney tissue collected by inducing euthanasia in rats. The sero-biochemical analysis revealed a significant increase in BUN and creatinine values of the rats in group 2. The antioxidant activity was analyzed and the rats in group 2 revealed a significant rise in the values of protein carbonyl, TBARS and significant decrease in GSH. Group 2 also showed an increase in TNF-α and decrease in interleukin-10 concentration. Sections of kidney tissue collected from group 2 showed marked dilation and elongation of tubules, moderate infiltration with inflammatory cells degeneration of bowman’s capsule and tubular congestion. Comparatively, groups undergone treatment showed amelioration in the parameters. Thus, resveratrol and vitamin-E exert protective actions against 5-flourouracil (5-FU) induced nephrotoxicity.