Ameliorating effect of the biological Zinc nanoparticles in abamectin induced hepato-renal injury in a rat model: Implication of oxidative stress, biochemical markers and COX-2 signaling pathways

Abstract

Extensive use of abamectin (ABM) as an anthelmintic in veterinary systems adversely affects the health and welfare of animals and humans. Zinc nanoparticles (ZnNPs) have therapeutic benefits and ameliorate the effect of environmental pollutants. In this study, we assessed the ameliorative effect of ZnNPs against the sub-lethal toxicity of ABM in rats. Forty healthy rats were randomly selected into four groups (n = 10); the control received normal saline and test rats were treated orally twice weekly with ABM (1 mg/kg bwt), ZnNPs (10 mg/kg bwt) and ABM + ZnNPs for 28 days. Upon completion of the study period, blood and tissue samples were collected and prepared for hematological, biochemical, pathological, and immunohistochemical analysis. Our results showed that ABM treatment significantly decreased body weight gain (BWG), red blood cells (RBCs), hemoglobin (Hb), hematocrit (HC), and platelet (PLT); while it significantly increased white blood cells (WBCs) and lymphocytes. ABM also significantly decreased antioxidant enzyme activities: superoxide dismuthase (SOD), glutathione peroxidase (GPx), and catalase (CAT) and increased hydrogen peroxide and malondialdehyde levels compared with other groups. ABM significantly raised alanine aminotransferase (ALT), aspartate amino transaminase (AST), and alkaline phosphatase (ALP) levels, which was restored by co-administration of ZnNPs. Moreover, ZnNPs ameliorated ABM-mediated negative histopathological changes in the liver and kidney tissues, exhibiting a significant protective effect. Cyclooxygenase 2 (COX-2) + immuno-expression were reduced after pretreatment with ZnNPs. These findings suggested that co-administration of ZnNPs with ABM mitigated its toxicity by combating oxidative stress and boosting antioxidant capacity, indicating the efficacy of ZnNPs in attenuating ABM toxicity.

Cinnamon nanoemulsion mitigates acetamiprid-induced hepatic and renal toxicity in rats: biochemical, histopathological, immunohistochemical, and molecular docking analysis

“…Also, Expression of COX-2 is associated with inflammation and is released by an array of proinflammatory stimuli [ 99 ]. Interestingly, a diffuse positive cytoplasmic expression of COX-2 illustrates the enormous cytoplasmic immunoreactivity for detecting COX-2 antigen in the livers and kidneys of abamectin-treated rats [ 100 ].…”

“…This may be because cinnamaldehyde can operate as a free radical scavenger, antioxidant, and anti-inflammatory agent [ 101 ]. Attaining notable downregulation of COX-2 enzyme expression during oxidative stress could be a critical strategy in regulating liver and kidney destruction [ 100 ]. Moreover, trans-cinnamaldehyde treatment uncovered anti-inflammatory actions by downregulating COX-2 expressions compared with methotrexate alone [ 102 ].…”

Use of Zinc Nanoparticles and/or Prodigiosin to Mitigate Heat Stress in Rabbits

“…In our previous investigation (Othman et al, 2019;Abdelnour et al, 2020), we isolated and identified the PRG from Serratia marcescens and lyophilized then the PRG was supplemented into diets conferring to the experiment protocol. For the zinc nanoparticles (ZnNPs), the Lactobacillus plantarum VITES07 was used for the biogenic ZnNPs, as described in our previous work (Aioub et al, 2022). Then, the TEM recognized the ZnNPs (transmission electron microscope, type JEOL-JEM 2100).…”

Hepatotoxicity of the Pesticide Avermectin Exposure to Freshwater-Farmed Carp: Evidence from In Vivo and In Vitro Research

“…14 Aioub et al demonstrated that alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase levels were increased after AVM stimulation in rats. 15 Furthermore, when lactating animals were exposed to AVM, a notable reduction in the body weight of the female subjects was observed. Simultaneously, the function of hepatic antioxidant enzymes experienced a decline, while the activity of lactate dehydrogenase (LDH) showed an escalation in female offspring.…”

“…Toxicological studies of AVM have shown that AVM intoxication usually causes an increase in liver–body ratio, hepatocyte edema with nuclear translocation . Aioub et al demonstrated that alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase levels were increased after AVM stimulation in rats . Furthermore, when lactating animals were exposed to AVM, a notable reduction in the body weight of the female subjects was observed.…”