Ameliorating Effect of Achillea fragrantissima Against Benzene-Induced Skin Irritation: Histological Study

Abstract

| Organic solvents such as car fuel, which contains benzene and other solvents were known to cause skin irritation and contact dermatitis. Although anti-inflammatory steroids topical medications were used, they may result in side effects in susceptible persons. Herbal preparation was tried in literature. In the present study, Achillea fragrantissima, a wild plant in some areas of Saudi Arabia was used to speed slow-healing wounds. Thus, the study was designed to investigate its efficacy to treat experimentally induced benzene-dermatitis in mice skin. Twenty-five adult mice were shaved and animals were divided into GI as control and GII as benzene painted. The later was further divided into four subgroups; untreated, steroidal medicinal cream treated, a group treated with olive oil extract of Achillea (dried plant soaked in olive oil) and a group treated with olive oil only. Gross and histopathological observation were used to evaluate the treatment effects. Benzene painting produced skin hardening, scaling, epidermal hyperplasia and inflammatory reaction in skin dermis. Decrease in the hypodermal adipose tissue thickness was observed, those pathological changes were much improved or even absent in treated mice. Such results are similar to that observed in the group treated by medicinal cream. Achillea plant could be a source of future effective anti-dermatitis cream medicinal formulation against benzene-induced dermatitis. Olive oil extract of Achillea will be tested and compared to olive oil alone to prove the hypothesis.

Efficacy of sweet pumpkin in relieving contact dermatitis in chronically stressed rats

“…In addition, there was a significant increase in the area percent of Masson-stained collagen fibers in the dorsal skin of rats of this group compared to the control group. These observations were in line with those previously described by Alshathly and Alqahtani 2017[ 28 ] in rats painted with benzene for 3 weeks.…”

Combined Oral and Topical Application of Pumpkin (Cucurbita pepo L.) Alleviates Contact Dermatitis Associated With Depression Through Downregulation Pro-Inflammatory Cytokines

“…The morphological and histopathological alternations observed in CD in this study were in accordance with those observed by Zhou et al (2016) in dinitrochlorobenzene-induced allergic CD in BALB/c mice (Alshathly and Alqahtani, 2017) in benzene-induced skin irritation in rats and (Qu et al, 2019) in oxazolone-induced CD in mice. Significant upregulation of Cox-2 immunoexpression in CD was among the findings recorded in this study and previous studies (Chang et al, 2018).…”