Amblyomma triste (Acari: Ixodidae): New North American Collection Records, Including the First From the United States

Mertins, James W.; Moorhouse, Arnold S.; Alfred, Jeffery T.; Hutcheson, H. Joel

doi:10.1093/jmedent/47.4.536

Cited by 36 publications

(22 citation statements)

References 24 publications

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“…However, Neumann (1899) considered that these three taxa belonged to a single species, namely A. maculatum, which was adopted by subsequent authors, until the study of Kohls (1956), who revalidated the three species based on morphological characters. Since then, although considered to be separate species, the identification of these tick species has been controversial, in particular the distinction between A. maculatum and A. triste (Estrada-Peña et al 2005;Guglielmone et al 2013;Lado 2015;Mendoza Uribe and Chavez Chorocco 2004;Mertins et al 2010). Lado (2015), based on different mitochondrial and nuclear molecular markers, suggests that A. triste should be synonymized with A. maculatum.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of germ cells and DNA of the genus Amblyomma: adding new data on Amblyomma maculatum and Amblyomma ovale species (Acari: Ixodidae)

et al. 2017

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Among tick species, members of the subfamily Amblyomminae have received special attention, since they serve as vectors for pathogens such as Rickettsia spp. and display cryptic species complexes that make their taxonomical classification challenging. Amblyomma ovale, Amblyomma maculatum, and other species of the genus Amblyomma have shown a long history of taxonomic controversies. Spermiotaxonomy has proved to be a valuable tool in the solution of systematic conflicts in Metazoa that can aid molecular and external morphological analyses in ticks and, overall, provide more robust analyses and results. With this in mind, this study included histological analyses of the reproductive system of the species A. ovale and A. maculatum, as well as the description of morphohistological characters of the male reproductive system of ticks of the genus Amblyomma, in order to evaluate these characters within the current clustering proposals. In addition, 16S rDNA and COI (mitochondrial) molecular markers were used to study the genetic relationships of the species. The results show that the tick male reproductive system and its germ cells contain useful candidate characters for taxonomical analyses of Ixodida.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis of germ cells and DNA of the genus Amblyomma: adding new data on Amblyomma maculatum and Amblyomma ovale species (Acari: Ixodidae)

et al. 2017

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“…Meanwhile, this limitation of the developed assay can be mitigated by its use in collection sites where A. triste is not likely present. The known distribution of A. triste within the United States is confined to the arid areas of Chihuahuan Desert and its fringes west of the 100th meridian (Mertins et al 2010). A. maculatum , however, is restricted to more humid habitats east of the 100th meridian.…”

Section: Discussionmentioning

confidence: 99%

“…Misidentification of closely related species using morphological keys is a relatively common problem. Mertins et al (2010), using molecular methods, found that some Amblyomma triste (Koch) ticks from different North American collections were misidentified as Amblyomma maculatum (Koch). Our own experience indicates that differentiating between look-alike Amblyomma cajennense (F.) and Amblyomma imitator (Kohls) may be a challenging task.…”

mentioning

confidence: 99%

Multiplex qPCR Assay for Identification and Differentiation ofAmblyomma americanum,Amblyomma cajennense, andAmblyomma maculatum(Ixodida: Ixodidae) Tick Species in the Eastern United States

2014

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Many ticks of the genus Amblyomma are vectors of human pathogens, and the correct species identification is medically and epidemiologically important. Morphological identification is time-consuming and requires a high level of expertise. Identification of engorged, immature, or damaged ticks and the differentiation of closely related species remain problematic. Here, we report the development of a real-time TaqMan assay for the genomic identification and differentiation of Amblyomma americanum (L.), Amblyomma cajennense (F.), and Amblyomma maculatum (Koch), which are human-biting species found in the eastern United States. New species-specific sets of oligonucleotides for the multiplex reaction that detect and differentiate the ITS2 genomic regions of three target species were designed using Visual OMP; the previously published A. americanum oligonucleotide set was also incorporated into our assay. Specificity and sensitivity tests for two multiplex master mixes using different A. americanum sets were performed using individual and pooled samples of adult, nymphal, and larval ticks, and optimization procedures were applied. The multiplex assay successfully differentiates between genomes of three target species and does not cross-react with DNAs of ticks from other genera. Rare cases of nonspecific amplification occurred with DNAs of A. imitator and Amblyomma triste Koch misidentified as A. americanum and A. maculatum, respectively. However, this cross-reaction does not diminish the usefulness of the developed assay east of the 95th meridian, where neither A. imitator nor A. triste are found. Two master mixes incorporating the previously published or newly developed A. americanum sets are being recommended for identification of individual ticks or pooled samples, respectively.

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“…Particularly high rates of R. parkeri infection (42–56%) in questing adult A. maculatum were detected in southeastern Virginia [ 22 ]. One confirmed and one probable case of R. parkeri rickettsiosis were acquired in southern Arizona and were likely vectored by Amblyomma triste ticks [ 60 ]; three ticks associated with this case cluster were PCR positive for R. parkeri [ 54 ].…”

Section: Tick-borne Rickettsiae (In Alphabetical Order)mentioning

confidence: 99%

Suspected and Confirmed Vector-Borne Rickettsioses of North America Associated with Human Diseases

Yoshimizu

Billeter

2018

TropicalMed

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The identification of pathogenic rickettsial agents has expanded over the last two decades. In North America, the majority of human cases are caused by tick-borne rickettsioses but rickettsiae transmitted by lice, fleas, mites and other arthropods are also responsible for clinical disease. Symptoms are generally nonspecific or mimic other infectious diseases; therefore, diagnosis and treatment may be delayed. While infection with most rickettsioses is relatively mild, delayed diagnosis and treatment may lead to increased morbidity and mortality. This review will discuss the ecology, epidemiology and public health importance of suspected and confirmed vector-transmitted Rickettsia species of North America associated with human diseases.

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Amblyomma triste (Acari: Ixodidae): New North American Collection Records, Including the First From the United States

Cited by 36 publications

References 24 publications

Comparative analysis of germ cells and DNA of the genus Amblyomma: adding new data on Amblyomma maculatum and Amblyomma ovale species (Acari: Ixodidae)

Comparative analysis of germ cells and DNA of the genus Amblyomma: adding new data on Amblyomma maculatum and Amblyomma ovale species (Acari: Ixodidae)

Multiplex qPCR Assay for Identification and Differentiation ofAmblyomma americanum,Amblyomma cajennense, andAmblyomma maculatum(Ixodida: Ixodidae) Tick Species in the Eastern United States

Suspected and Confirmed Vector-Borne Rickettsioses of North America Associated with Human Diseases

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