Ambiguous effect of dendrimer PAMAM G3 on rat heart respiration in a model of an experimental diabetes – Objective causes of laboratory misfortune or unpredictable G3 activity?

Siewiera, Karolina; Łabieniec-Watała, Magdalena

doi:10.1016/j.ijpharm.2012.03.037

Cited by 15 publications

(19 citation statements)

References 29 publications

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“…Interestingly, we have noticed, although not for the first time [16,17], that the response of mitochondria to the treatment with various compounds depends on the seasonality. The results presented in this paper also indicate that there is a statistically significant difference between the activities of the mitochondria isolated from rats investigated in autumn (October-November) and the mitochondria isolated from animals studied in spring (May-June).…”

Section: Discussionmentioning

confidence: 78%

The Impact of Seasonal Fluctuations on Rat Liver Mitochondria Response to Tested Compounds— A Comparison between Autumn and Spring. New Insight into Collecting and Interpretation of Experimental Data Originating from Different Seasons

Łabieniec-Watała¹,

Siewiera²

2013

CellBio

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Seasonal variations play an essential role in the metabolism, behavior and activity of the laboratory animals. This study was aimed to examine whether mitochondrial function can be influenced by the seasonal changes and how large is the impact of these fluctuations on experiments with using animal models and further results interpretation. Liver mitochondria were isolated from male Wistar rats and exposed to calcium ions, PAMAM dendrimers G2.5 or their combination: (Ca 2+ ) and dendrimer. The scientific hypothesis assumed that dendrimer G2.5 is able to limit the detrimental effect of Ca 2+ ions on mitochondria function, possibly through affecting the following parameters: calcium transport, mitochondrial potential and membrane fluidity. The activity of mitochondria was monitored using fluorescent labels. The changes in calcium transport were detected using Calcium Green 5-N, the mitochondrial membrane potential and membrane fluidity were elucidated using JC-1 and DPH, respectively. The experiments were carried out during the autumn (October/November) or during the spring (May/June). The obtained data emphasize the effect of seasonal differences on liver mitochondria originating from laboratory animals and outline the importance of planning the experiments during the same seasonal period in order to receive objective and reliable results in the future. Finally, it was revealed the neutral effect of G2.5 dendrimer on mitochondria and its inability to protect mitochondria against overload of calcium ions regardless of seasonality. It was also evidenced that liver mitochondria isolated from autumn-derived animals were more sensitive to calcium and/or dendrimer exposure in comparison with mitochondria isolated from animals investigated during the spring.

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Section: Discussionmentioning

confidence: 78%

The Impact of Seasonal Fluctuations on Rat Liver Mitochondria Response to Tested Compounds— A Comparison between Autumn and Spring. New Insight into Collecting and Interpretation of Experimental Data Originating from Different Seasons

Łabieniec-Watała¹,

Siewiera²

2013

CellBio

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“…Further study revealed that G3 dendrimer activity could be toxic with higher mortality ranges. The author's view supports experimentation with animal models as in vitro studies does not completely relate with live animal models, and the outcome of the two could be anomalous [42].…”

Section: Modulating Mitochondrial Function and Protectionmentioning

confidence: 99%

“…Mitochondria are known to play a vital role in insulin resistance and diabetes pathogenesis [42]. In diabetes, there is a decrease in efficiency of mitochondrial respiration especially in tissues like brain and heart cells that are highly dependent on aerobic metabolism.…”

Section: Modulating Mitochondrial Function and Protectionmentioning

confidence: 99%

“…This decreased efficiency is due to disturbance in mitochondrial functioning, reduction in electron transport chain, which in turn lowers the ATP output. Reduced ATP formation decreased oxidative capacity and increased reactive oxygen species (ROS) production are common characteristics of diabetic heart [42].…”

Section: Modulating Mitochondrial Function and Protectionmentioning

confidence: 99%

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Dendritic platforms for biomimicry and biotechnological applications

Nagpal

Mohan

Thakur

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Dendrimers, commonly referred to as polymeric trees, offer endless opportunities for biotechnological and biomedical applications. By controlling the type, length, and molecular weight of the core, branches and end groups, respectively, the chemical functionality and topology of dendrimeric archetypes can be customized which further can be applied to achieve required solubility, biodegradability, diagnosis and other applications. Given the physicochemical variability of the dendrimers and their hybrids, this review attempts to discuss a full spectrum of recent advances and strides made by these "perfectly designed structures". An extensive biotech/biomimicry application profiling of dendrimers is provided with focus on complex archetypical designs such as protein biomimicry (angiogenic inhibitors, regenerative hydroxyapatite and collagen) and biotechnology applications. In terms of biotechnological advances, dendrimers have provided distinctive advantages in the fields of biocatalysis, microbicides, artificial lights, mitochondrial function modulation, vaccines, tissue regeneration and repair, antigen carriers and even biosensors. In addition, this review provides overview of the extensive chemo-functionalization opportunities available with dendrimers which makes them a perfect candidate for forming drug conjugates, protein hybrids, bio mimics, lipidic derivatives, metal deposits and nanoconjugates thereby making them the most multifunctional platforms for diverse biotechnological applications.

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“…A considerable inconsistency and/or variability of outcomes originating from studies on animals may be simply because of the diversity of the used animal models and strains, differentiated treatment durations, and/or the phenomenon of seasonal fluctuations. It has been noticed and emphasized earlier that when working with laboratory animals, the phenomenon of seasonal fluctuations should certainly be taken into consideration, as such fluctuations can potentially affect animal metabolism and bioenergetics, also under a standard housing regime . Another important point concerns the use of different laboratory rodent strains to study similar models of pathology.…”

Section: Limitations and Restraints In Pamam Applications—unjustifiedmentioning

confidence: 99%

PAMAM Dendrimers: Destined for Success or Doomed to Fail? Plain and Modified PAMAM Dendrimers in the Context of Biomedical Applications

Łabieniec-Watała

Watała

2015

Journal of Pharmaceutical Sciences

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116

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Ambiguous effect of dendrimer PAMAM G3 on rat heart respiration in a model of an experimental diabetes – Objective causes of laboratory misfortune or unpredictable G3 activity?

Cited by 15 publications

References 29 publications

The Impact of Seasonal Fluctuations on Rat Liver Mitochondria Response to Tested Compounds— A Comparison between Autumn and Spring. New Insight into Collecting and Interpretation of Experimental Data Originating from Different Seasons

The Impact of Seasonal Fluctuations on Rat Liver Mitochondria Response to Tested Compounds— A Comparison between Autumn and Spring. New Insight into Collecting and Interpretation of Experimental Data Originating from Different Seasons

Dendritic platforms for biomimicry and biotechnological applications

PAMAM Dendrimers: Destined for Success or Doomed to Fail? Plain and Modified PAMAM Dendrimers in the Context of Biomedical Applications

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