Askwith T, Zeng W, Eggo MC, Stevens MJ. Oxidative stress and dysregulation of the taurine transporter in high-glucose-exposed human Schwann cells: implications for pathogenesis of diabetic neuropathy. Am J Physiol Endocrinol Metab 297: E620 -E628, 2009. First published July 14, 2009; doi:10.1152/ajpendo.00287.2009.-In human Schwann cells, the role of taurine in regulating glucose-induced changes in antioxidant defense systems has been examined. Treatment with high glucose for 7 days induced reactive oxygen species, increased 4-hydroxynoneal adducts (20 Ϯ 5%, P Ͻ 0.05) and poly-(ADP-ribosyl)ated proteins (40 Ϯ 13%, P Ͻ 0.05). Increases in these markers of oxidative stress were reversed by simultaneous incubation in 0.25 mM taurine. Both high glucose and taurine independently increased superoxide dismutase and catalase activity and decreased glutathione levels, but their effects were not additive. Glucose reduced taurine transporter (TauT) mRNA and protein in a dose-dependent manner with maximal decreases of 66 Ϯ 6 and 63 Ϯ 12%, respectively (P Ͻ 0.05 both). The Vmax for taurine uptake was decreased in 30 mM glucose from 61 Ϯ 5 to 42 Ϯ 3 pmol ⅐ min Ϫ1 ⅐ mg protein
Ϫ1(P Ͻ 0.001). Glucose-induced TauT downregulation could be reversed by inhibition of aldose reductase, a pathway that depletes NADPH and increases osmotic stress and protein glycation. TauT protein was increased more than threefold, and the Vmax for taurine uptake doubled (P Ͻ 0.05 both) by prooxidants. TauT downregulation was reversed both by treatment with the antioxidant ␣-lipoic acid, which increased TauT mRNA by 60% and Vmax by 50% (P Ͻ 0.05 both), and by the aldose reductase inhibitor sorbinil, which increased TauT mRNA 380% and Vmax by 98% (P Ͻ 0.01 both). These data highlight the potential therapeutic benefits of taurine supplementation in diabetic complications and provide mechanisms whereby taurine restoration could be achieved in order to prevent or reverse diabetic complications.THE RELATIONSHIP OF HYPERGLYCEMIA to the microvascular complications of diabetes is well established (13a, 76a, 76b), but the mechanisms contributing to the development and progression of complications are not well understood. Recently, increased oxidative/nitrosative stress has been implicated as a key pathogenetic pathway (9,18,62,78). Increased oxidative stress has been identified in the nerve (4, 24, 68), eye (54), vasculature (31), kidney (3, 32), and heart (29) in diabetic rodent models and in diabetic patients (23,25,43,44). Antioxidant approaches have been shown to prevent or reverse complications in experimental diabetes (50). Glucose-driven oxidative stress occurs due to an imbalance in the production of reactive oxygen species (ROS) in concert with impaired antioxidant defense. Excess ROS production can have many downstream pathogenic effects within the cell, including protein nitrosylation, formation of poly(ADP-ribosyl)ated (PAR) protein polymers, and apoptosis (22,35,49,51,56,63). Flux through the enzyme aldose reductase (AR) pathway at key sites for di...