Amaryllis and Shrimp plant are secondary hosts of Cucumber mosaic cucumovirus (CMV) in Iraq

Adhab, Mustafa; Al-Ani, R. A.

doi:10.5251/abjna.2011.2.5.872.875

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…Infecting more than 1200 dicotyledonous and monocotyledonous plant species, cucumber mosaic virus (CMV; genus Cucumovirus, family Bromoviridae) is one of the most important viruses worldwide [10,27,30]. CMV induces various kinds of symptoms, which depend on the host plant species and virus strain, ranging from little or no visible symptoms to severe mosaic associated with stunting and leaf deformations [2,4,12,15,20,23,28].…”

Section: Introductionmentioning

confidence: 99%

The 2b protein and C-terminal region of the 2a protein indispensably facilitate systemic movement of cucumber mosaic virus in radish with supplementary function by either the 3a or the coat protein

Khaing

Kobayashi

Takeshita

2020

Virol J

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Background: In Raphanus sativus (Japanese radish), strain D8 of cucumber mosaic virus (CMV-D8) establishes a systemic infection and induces mild mosaic on upper, non-inoculated leaves, whereas strain Y of CMV (CMV-Y) causes only a local infection in the inoculated leaves. Here, we further analyzed the specific viral factor(s) of CMV-D8 that is (are) indispensable for systemic infection in Japanese radish. Methods: To identify which genomic RNA(s) is (are) involved in systemic infection in radish, we carried out a pseudorecombination analysis between CMV-D8 and CMV-Y. With recombination analyses between CMV-D8 and CMV-Y using mutant/recombinant RNA2s, chimeric and point-mutated RNA3s, we identified viral factors that are indispensable for systemic infection. Results: Viral RNA2 and RNA3 of CMV-D8 facilitated efficient virus spread into the upper, non-inoculated plant tissues of radish (cv. Tokinashi), but not those of CMV-Y. Recombinant RNA2s demonstrated that the 2b protein (2b) and the C-terminus of the 2a protein (2a) of CMV-D8 have a crucial role in systemic infection. In addition, we used chimeric and point-mutated RNA3s to that Pro 17 and Pro 129 in the coat protein (CP) of CMV-D8 are involved in efficient systemic infection and that Ser 51 in the 3a protein (3a) of CMV-D8 has positive effects on systemic spread. The results suggested that these viral factors facilitate systemic infection of CMV-D8 in Japanese radish. Conclusion: The C-terminal region of 2a, the entire region of 2b, and supplementary function of either Ser 51 in 3a or Pro 17 /Pro 129 in CP confer systemic infectivity on CMV-D8 in radish. These results further elucidate the complex interaction of viral proteins of CMV to complete systemic infection as a host-specific manner.

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