Alveolar epithelial transport in the adult lung

Dobbs, Leland G.; Johnson, Meshell

doi:10.1016/j.resp.2007.06.011

Cited by 53 publications

(51 citation statements)

References 166 publications

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“…AT1 cells are large thin cells that cover a large part of the alveolar surface area. AT2 cells are cuboidal in shape and comprise 5% of the alveolar surface area, yet they constitute 60% of alveolar epithelial cells (3)(4)(5)(6). Because AT2 cells can be isolated, cultured, and studied in vitro, they have been used in attempts to understand their contribution to alveolar liquid balance.…”

Section: From the Department Of Medicine Cystic Fibrosis/pulmonary Rmentioning

confidence: 99%

Human Alveolar Type II Cells Secrete and Absorb Liquid in Response to Local Nucleotide Signaling

Bove

Grubb

Okada

et al. 2010

Journal of Biological Chemistry

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A balance sheet describing the integrated homeostasis of secretion, absorption, and surface movement of liquids on pulmonary surfaces has remained elusive. It remains unclear whether the alveolus exhibits an intra-alveolar ion/liquid transport physiology or whether it secretes ions/liquid that may communicate with airway surfaces. Studies employing isolated human alveolar type II (AT2) cells were utilized to investigate this question. Human AT2 cells exhibited both epithelial Na

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Section: From the Department Of Medicine Cystic Fibrosis/pulmonary Rmentioning

confidence: 99%

Human Alveolar Type II Cells Secrete and Absorb Liquid in Response to Local Nucleotide Signaling

Bove

Grubb

Okada

et al. 2010

Journal of Biological Chemistry

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“…The alveolar epithelium is composed of alveolar epithelial type I (ATI) and type II (ATII) cells. ATII cells are cuboidal secretory cells mainly responsible for surfactant secretion, thereby reducing surface tension [2]. In addition, ATII cells have been implicated in lung defense mechanisms due to cytokine release and antioxidant production.…”

Section: Introductionmentioning

confidence: 99%

Lung Cancer in Pulmonary Fibrosis: Tales of Epithelial Cell Plasticity

Königshoff

2011

Respiration

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Lung epithelial cells exhibit a high degree of plasticity. Alterations to lung epithelial cell function are critically involved in several chronic lung diseases such as pulmonary fibrosis. Pulmonary fibrosis is characterized by repetitive injury and subsequent impaired repair of epithelial cells, which leads to aberrant growth factor activation and fibroblast accumulation. Increased proliferation and hyper- and metaplasia of epithelial cells upon injury have also been observed in pulmonary fibrosis; this epithelial cell activation might represent the basis for lung cancer development. Indeed, several studies have provided histopathological evidence of an increased incidence of lung cancer in pulmonary fibrosis. The mechanisms involved in the development of cancer in pulmonary fibrosis, however, remain poorly understood. This review highlights recently uncovered molecular mechanisms shared between lung cancer and fibrosis, which extend the current evidence of a common trait of cancer and fibrosis, as provided by histopathological observations.

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“…The Na + /K + ATPase has been found to be expressed in the basolateral membrane of alveolar epithelial type I cells of rat lung (Borok et al 2002;Johnson et al 2002; for a review see Dobbs and Johnson 2007), besides in that of type II cells (Schneeberger and McCarthy 1986). Hence, also alveolar type I cells are provided with both molecules required to produce a Na + -glucose transport, and the coupled liquid absorption through the alveolar epithelium.…”

Section: Discussionmentioning

confidence: 99%

Evidence for Na+–glucose cotransporter in type I alveolar epithelium

Bodega

Sironi

Armilli

et al. 2010

Histochem Cell Biol

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Functional evidence of Na(+)-glucose cotransport in rat lung has been provided by Basset et al. (J. Physiol. 384:325-345, 1987). By autoradiography [(3)H]phloridzin binding has been found confined to alveolar epithelial type II cells in mouse and rabbit lungs (Boyd, J. Physiol. 422: 44P, 1990). In this research we checked by immunofluorescence whether Na(+)-glucose cotransporter (SGLT1) is also expressed in alveolar type I cells. Lungs of anesthetized rats and lambs were fixed by paraformaldehyde, perfused in pulmonary artery, or instilled into a bronchus, respectively. Tissue blocks embedded in paraffin or frozen were sectioned. Two specific anti-SGLT1 antibodies for rat recognizing aminoacid sequence 402-420, and 546-596 were used in both species. Bound primary antibody was detected by secondary antibody conjugated to fluorescein isothiocianate or Texas red, respectively. In some sections cellular nuclei were also stained. In rats alveolar type I cells were identified by fluorescent Erythrina cristagalli lectin. Sections were examined by confocal laser-scanning microscope. Both in rats and lambs alveolar epithelium was stained by either antibody; no labeling occurred in negative controls. Hence, SGLT1 appears to be also expressed in alveolar type I cells. This is functionally relevant because type I cells provide 95-97% of alveolar surface, and SGLT1, besides contributing to removal of lung liquid under some circumstances, keeps low glucose concentration in lining liquid, which is useful to prevent lung infection.

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Alveolar epithelial transport in the adult lung

Cited by 53 publications

References 166 publications

Human Alveolar Type II Cells Secrete and Absorb Liquid in Response to Local Nucleotide Signaling

Human Alveolar Type II Cells Secrete and Absorb Liquid in Response to Local Nucleotide Signaling

Lung Cancer in Pulmonary Fibrosis: Tales of Epithelial Cell Plasticity

Evidence for Na+–glucose cotransporter in type I alveolar epithelium

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