Alveolar Echinococcosis of the Liver

Claudon, M.; Bessieres, Michel; Régent, D; Rodde, A; Bazin, Christophe; Gérard, Alain; Bresler, L.

doi:10.1097/00004728-199007000-00018

Cited by 37 publications

(23 citation statements)

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“…During the examinations, the animals were anesthetized with 1.5% isoflurane in O 2 , and respiration was monitored. Imaging was performed using flash 3D vibe (TE/TR 1.14/20 ms, FoV 59×70 mm 2 , matrix of 162×192, slice thickness 0.36 mm) and T2-weighted turbo-spin echo (TE / TR = 40/3000 ms, FoV ¼ 60 Â 60mm 2 , matrix of 320×320, slice thickness 1.5 mm) sequences for volume measurements and detection of extrahepatic and vascular infestation, characterization of multivesicular structures of the metacestodes, as well as delineation of necrotic host and parasite tissue as it has been detected previously in human cases (Claudon et al 1990;Duewell et al 1990). The scanner operates under the syngo MRI software (Siemens Medical Solutions, Germany) for image acquisition, and Inveon Research Workplace software (Siemens Preclinical Solutions, Knoxville, Tennessee, USA) was used to analyze the MR images and to perform volumetry.…”

Section: Bioassaysmentioning

confidence: 99%

Effects of in vitro exposure of Echinococcus multilocularis metacestodes to cytostatic drugs on in vivo growth and proliferation of the parasite

Hübner¹,

Wiehr

Kocherscheidt³

et al. 2010

Parasitol Res

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The cytostatic drugs Vincristine (VCR), Navelbine (NAV), and Methotrexate (MTX) were evaluated for their growth inhibitory potential against metacestodes of Echinococcus multilocularis (Em) by in vitro and in vivo assays. In vitro cultures of E. multilocularis were exposed to IC 90, IC 80, and IC 5 concentrations of VCR, NAV, or MTX for 1 week, then parasite tissue cultures were kept for 1 week without drug exposure in vitro, and thereafter, metacestode tissues were injected intra-peritoneally into Meriones unguiculatus. Metacestode growth was monitored for several months post-infection (p.i.) by body weight control, magnetic resonance imaging (MRI), and autopsy at 5 months p.i. Weight monitoring of infected M. unguiculatus did not provide conclusive evidence for Em-metacestode growth, while MRI could detect growing Em-metacestode in the MTX-treated group at 8 weeks (p.i.), whereas metacestodes exposed to VCR and NAV were at 17 weeks (p.i.) detectable. MRI disclosed progressive and massive growth of Em-metacestode in the VCR- and MTX-exposed groups, while the NAV-pretreated Em-metacestodes' volume did not exceed 4 cm(3). At autopsy, Em-metacestodes of less than 4 cm(3) were found in infected M. unguiculatus, which was not detected by MRI. In summary, the cytostatic drugs Methotrexate, Navelbine, and Vincristine--as applied in the present work--did not show parasitocidal or clear parasitostatic effects on metacestodes of E. multilocularis. While parasite growth in vivo was inhibited in NAV- and VCR-pretreated Em-metacestodes, MTX pretreatment seemed to enhance parasite proliferation. Magnetic resonance imaging appears suitable to monitor in vivo the effects of drugs on growth progression and regression only of larger Em-metacestode tissues.

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Section: Bioassaysmentioning

confidence: 99%

Effects of in vitro exposure of Echinococcus multilocularis metacestodes to cytostatic drugs on in vivo growth and proliferation of the parasite

Hübner¹,

Wiehr

Kocherscheidt³

et al. 2010

Parasitol Res

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“…Magnetic resonance imaging (MRI) has formerly played only a minor role in the diagnosis of hepatic lesions (including AE) due to higher costs, longer time of investigation and restricted availability [9,10].…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies comparing the above imaging techniques showed that no imaging technique was uniquely superior [9][10][11][12][13]. US proved advantageous due to its ubiquitous availability, easy handling, cost-effectiveness and lack of radiation.…”

Section: Introductionmentioning

confidence: 99%

“…US proved advantageous due to its ubiquitous availability, easy handling, cost-effectiveness and lack of radiation. Calcifications are often observed in AE lesions and may be considered as pathognomonic for AE; they are best detected with CT [7,9] and CT is currently considered to be the gold standard in AE imaging. MRI, however, does not use radiation and was shown to best depict extrahepatic and vascular affection [9,10,14].…”

Section: Introductionmentioning

confidence: 99%

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Alveolar Liver Echinococcosis: A Comparative Study of Three Imaging Techniques

et al. 2001

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US is the screening method of choice and should primarily be complemented by CT due to its ability to detect the greatest number of lesions and clear demarcation of the characteristic calcifications. MRI may facilitate the diagnosis in uncertain cases with noncalcified or partially calcified lesions by showing the characteristic multivesicular structure, necrotic areas and proximity to vascular structures.

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“…While these modalities mostly provide satisfactory information about the disease in endemic areas, as in ours, further diagnostic investigative tools, such as biopsies, may be needed in non-endemic regions [7][8][9].…”

Section: Discussionmentioning

confidence: 81%