Alveolar-Capillary Region Exposure (ACRE) Model

Abstract

This exposure model was designed as an in vitro representation of trans-alveolar effects of airway particulate matter exposures on microvascular endothelial cells in the alveolar capillary region of the lung. The alveolar epithelial cells are represented by the NCI-H441 cell line, interstitial fibroblasts are represented by the IMR90 cell line, and microvascular endothelial cells are represented by the HULEC cell line. Disclaimer: The contents of this article have been reviewed by the US Environmental Protection Agency and approved for publication and do not necessarily represent Agency policy. Mention of trade names or commercial products does not constitute endorsement or recommendations for use. NOTE: There is a consolidated PDF version of this protocol in the supplementary files section below.

Characterizing the extracellular vesicle proteomic landscape of the human airway using in vitro organotypic multi-cellular models

“…The tri-culture and co-culture models were setup following the “Alveolar Capillary Region Exposure (ACRE) Exposure Model” methodology, detailed in full through the online, publicly available Nature Portfolio, Protocol Exchange 73 up to Day 3. On day 3 of the tri-culture model setup, HULECs were seeded in separate collagen-coated 69 multi-well plates in complete HULEC growth medium and incubated in a tissue culture incubator for 9 h to permit the formation of a confluent monolayer.…”