Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis

Hassumi, Jaqueline Suemi; Mulinari‐Santos, Gabriel; Fábris, André Luís da Silva; Jacob, Ricardo Garcia Mureb; Goncalves, Aline; Rossi, Ana Cláudia; Freire, Alexandre Rodrigues; Faverani, Leonardo Pérez; Okamoto, Roberta

doi:10.1590/1678-7757-2017-0326

Cited by 60 publications

(46 citation statements)

References 27 publications

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“…Using rat as an experimental animal has many advantages including its availability, economic expenses, and healing short duration, since females are more prone for osteoporosis than males, males were chosen in the present study. This in agreement with Hassumi et al 2018 (24) who studied alveolar bone healing in rats. While, Abegao et al 2015 found that using rabbits as a model in extraction wound is better as a larger animal and also rapid healing cycle of its bone in comparison to human (25).…”

Section: Discussionsupporting

confidence: 91%

Scannining Electron Microscopic Evaluation of the Effect of Boron and Fish Oil on Healing of Extraction Sockets in Rats

AbdElfatah

Samad

Koura

et al. 2020

Alexandria Dental Journal

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INTRODUCTION: Great incidence of complications have been reported tooth extraction. Many materials and drugs were used previously to cover extraction socket in an attempt to enhance healing or prevent post-operative complications. Several studies suggested that Boron and fish oil affect many of the biological processes during bone healing by influencing cell membrane function. Thus, they affect cellular responses to cytokines and hormones, including those involved in bone growth and turnover in rats. OBJECTIVE: of the study: To evaluate the effect of boron and fish oil on alveolar bone healing after tooth extraction in rats using Scanning Electron Microscope, Energy Dispersive x-ray analysis [EDXA] and serum alkaline phosphatase analysis. MATERIALS AND METHODS: forty-five adult male albino rats (120-150 gm in weight) were used in this study. Extraction of the lower 1 st molar was performed in all animals then they were randomly divided into 3 equal groups; Group I (control) including 15 rats fed on normal diet, Group II (boron) including 15 rats receiving normal diet and boron supplementation (3 mg/kg boron diluted in 3.5 ± 4 ml distilled water) daily, Group III (fish oil) including 15 rats receiving normal diet and fish oil supplementation (65 mg/kg fish oil) daily. The animals were sacrificed after 2 weeks and the effect of boron and fish oil was evaluated using Scanning Electron Microscope, Energy Dispersive x-ray analysis and Serum alkaline phosphatase level analysis. The data obtained was tabulated and subjected to statistical analysis. RESULTS: After 2 weeks of extraction, scanning Electron Microscopic results revealed an increase in trabecular bone thickness with regular bone surface, and uniform osteocyte lacunae in Group II (boron) in comparison to group I (control) and Group III (fish oil) and these results were proved by EDXA which showed increase in Ca level in group II than group I and group III. On the other hand, serum alkaline phosphatase analysis showed that group III (fish oil) has the higher level of ALP after 2 weeks of the experiment. CONCLUSION: Boron and fish oil systemic administration have a positive effect on socket healing and bone structure and composition in rats.

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Section: Discussionsupporting

confidence: 91%

Scannining Electron Microscopic Evaluation of the Effect of Boron and Fish Oil on Healing of Extraction Sockets in Rats

AbdElfatah

Samad

Koura

et al. 2020

Alexandria Dental Journal

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“…The immunohistochemical reactions were evaluated by assigning scores based on a semi-quantitative analysis [ 31 , 32 , 33 , 34 , 35 , 36 , 37 ]. The scores were assigned as null (0), mild (1), moderate (2), and intense (3).…”

Section: Methodsmentioning

confidence: 99%

Is the Bacterial Cellulose Membrane Feasible for Osteopromotive Property?

et al. 2020

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Guided bone regeneration was studied to establish protocols and develop new biomaterials that revealed satisfactory results. The present study aimed to comparatively evaluate the efficiency of the bacterial cellulose membrane (Nanoskin®) and collagen membrane Bio-Gide® in the bone repair of 8-mm critical size defects in rat calvaria. Seventy-two adult male rats were divided into three experimental groups (n = 24): the CG—membrane-free control group (only blood clot, negative control), BG—porcine collagen membrane group (Bio-Guide®, positive control), and BC—bacterial cellulose membrane group (experimental group). The comparison periods were 7, 15, 30, and 60 days postoperatively. Histological, histometric, and immunohistochemical analyses were performed. The quantitative data were subjected to 2-way ANOVA and Tukey’s post-test, and p < 0.05 was considered significant. At 30 and 60 days postoperatively, the BG group showed more healing of the surgical wound than the other groups, with a high amount of newly formed bone (p < 0.001), while the BC group showed mature connective tissue filling the defect. The inflammatory cell count at postoperative days 7 and 15 was higher in the BC group than in the BG group (Tukey’s test, p = 0.006). At postoperative days 30 and 60, the area of new bone formed was greater in the BG group than in the other groups (p < 0.001). Immunohistochemical analysis showed moderate and intense immunolabeling of osteocalcin and osteopontin at postoperative day 60 in the BG and BC groups. Thus, despite the promising application of the BC membrane in soft-tissue repair, it did not induce bone repair in rat calvaria.

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“…The level of significance of 5% and a power test of 80% was used to calculate the sample size used in this study [Sealedenvelope. Available online: ] (Adapted from Hassumi et al, 2018) [ 18 ]. The result suggested five animals in each group.…”

Section: Methodsmentioning

confidence: 99%

In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes

et al. 2020

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Mechanical barriers prevent the invasion of the surrounding soft tissues within the bone defects. This concept is known as Guided Bone Regeneration (GBR). The knowledge about the local tissue reaction and the time of degradation of absorbable membranes favors the correct clinical indication. This study aimed to evaluate the biocompatibility and biodegradation of a bovine collagen membrane (Lyostypt®, São Gonçalo, Brazil) and compare it to a porcine collagen membrane (Bio-Gide®) implanted in the subcutaneous tissue of mice, following ISO 10993-6:2016. Thirty Balb-C mice were randomly divided into three experimental groups, LT (Lyostypt®), BG (Bio-Gide®), and Sham (without implantation), and subdivided according to the experimental periods (7, 21, and 63 days). The BG was considered non-irritant at seven days and slight and moderate irritant at 21 and 63 days, respectively. The LT presented a small irritant reaction at seven days, a mild reaction after 21, and a reduction in the inflammatory response at 63 days. The biodegradation of the LT occurred more rapidly compared to the BG after 63 days. This study concluded that both membranes were considered biocompatible since their tissue reactions were compatible with the physiological inflammatory process; however, the Bio-Gide® was less degraded during the experimental periods, favoring the guided bone regeneration process.

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Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis

Cited by 60 publications

References 27 publications

Scannining Electron Microscopic Evaluation of the Effect of Boron and Fish Oil on Healing of Extraction Sockets in Rats

Scannining Electron Microscopic Evaluation of the Effect of Boron and Fish Oil on Healing of Extraction Sockets in Rats

Is the Bacterial Cellulose Membrane Feasible for Osteopromotive Property?

In Vivo Comparative Evaluation of Biocompatibility and Biodegradation of Bovine and Porcine Collagen Membranes

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