Alveolar bone healing in mice genetically selected in the maximum (AIRmax) or minimum (AIRmin) inflammatory reaction

Colavite, Priscila María; Vieira, Andreia; Repeke, Carlos; Linhari, Rafaella Pavanelli de Araujo; Andrade, Raíssa Gonçalves Carneiro Spera De; Borrego, Andrea; Franco, Marcelo De; Trombone, Ana Paula; Garlet, Gustavo

doi:10.1016/j.cyto.2018.11.027

Cited by 8 publications

(11 citation statements)

References 81 publications

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“…A cylindrical region of interest (ROI) with an axis length of 3 mm and a diameter of 1 mm was determined by segmenting the trabecular bone located from the coronal to apical thirds. Measurements included the tissue volume (TV), bone volume (BV), bone volume fraction (BV/TV, %), trabecular thickness (Tb.Th, mm), trabecular number (Tb.N, mm) and trabecular separation (Tb.Sp) (25,(45)(46)(47).…”

Section: Micro-computed Tomography (Mct) Assessmentmentioning

confidence: 99%

“…For each animal/socket, sections from the medial were evaluated. In the morphometric analysis, points were counted coinciding with the images of the following components of the alveolar socket: clot, inflammatory cells, blood vessels, fibroblasts, collagen fibers, bone matrix, osteoblasts, osteoclasts and other components (empty space left by extracellular liquid and bone marrow); similar to previous descriptions (25,(47)(48)(49)(50). The results were presented as the mean of volume density for each evaluated structure.…”

Section: Histomorphometric Analysismentioning

confidence: 99%

“…The Picrosirius-polarization stain method and the quantification of birefringent fibers were performed to assess the structural changes in the newly formed bone trabeculae matrix, based on the birefringence of the collagen fiber bundles as previously described (25,47,51,52). Serial sections (8 semi-serial sections of each maxilla) with 5 mm thickness were cut and stained by Picrosirius Red; all sections were stained simultaneously to avoid variations due to possible differences in the staining process.…”

Section: Collagen Birefringence Analysismentioning

confidence: 99%

“…Real Time PCR array reactions were performed as previously described (25,47). In short, extraction of total RNA from the remaining alveolus was performed with the RNeasy Plus kit (Qiagen Inc, Valencia, CA) according to the manufacturer's instructions.…”

Section: Real Time Pcr Array Reactionsmentioning

confidence: 99%

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Macrophage Polarization and Alveolar Bone Healing Outcome: Despite a Significant M2 Polarizing Effect, VIP and PACAP Treatments Present a Minor Impact in Alveolar Bone Healing in Homeostatic Conditions

et al. 2021

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Host inflammatory immune response comprises an essential element of the bone healing process, where M2 polarization allegedly contributes to a favorable healing outcome. In this context, immunoregulatory molecules that modulate host response, including macrophage polarization, are considered potential targets for improving bone healing. This study aims to evaluate the role of the immunoregulatory molecules VIP (Vasoactive intestinal peptide) and PACAP (Pituitary adenylate cyclase activating polypeptide), which was previously described to favor the development of the M2 phenotype, in the process of alveolar bone healing in C57Bl/6 (WT) mice. Experimental groups were submitted to tooth extraction and maintained under control conditions or treated with VIP or PACAP were evaluated by microtomographic (µCT), histomorphometric, immunohistochemical, and molecular analysis at 0, 3, 7, and 14 days to quantify tissue healing and host response indicators at the healing site. Gene expression analysis demonstrates the effectiveness of VIP or PACAP in modulating host response, evidenced by the early dominance of an M2-type response, which was paralleled by a significant increase in M2 (CD206+) in treated groups. However, despite the marked effect of M1/M2 balance in the healing sites, the histomorphometric analysis does not reveal an equivalent/corresponding modulation of the healing process. µCT reveals a slight increase in bone matrix volume and the trabecular thickness number in the PACAP group, while histomorphometric analyzes reveal a slight increase in the VIP group, both at a 14-d time-point; despite the increased expression of osteogenic factors, osteoblastic differentiation, activity, and maturation markers in both VIP and PACAP groups. Interestingly, a lower number of VIP and PACAP immunolabeled cells were observed in the treated groups, suggesting a reduction in endogenous production. In conclusion, while both VIP and PACAP treatments presented a significant immunomodulatory effect with potential for increased healing, no major changes were observed in bone healing outcome, suggesting that the signals required for bone healing under homeostatic conditions are already optimal, and additional signals do not improve an already optimal process. Further studies are required to elucidate the role of macrophage polarization in the bone healing process.

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Section: Micro-computed Tomography (Mct) Assessmentmentioning

confidence: 99%

Section: Histomorphometric Analysismentioning

confidence: 99%

Section: Collagen Birefringence Analysismentioning

confidence: 99%

Section: Real Time Pcr Array Reactionsmentioning

confidence: 99%

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Macrophage Polarization and Alveolar Bone Healing Outcome: Despite a Significant M2 Polarizing Effect, VIP and PACAP Treatments Present a Minor Impact in Alveolar Bone Healing in Homeostatic Conditions

et al. 2021

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“…During the inflammation process following the traumatic stimulus immediately a large number of mediators, e.g. factors such as IL-1β, IL-6, IL-8, CCL2 and TNFα are expressed [ 9 – 12 ]. Nevertheless compared to soft tissue repair reactions the inflammatory process is then downregulated in the early phase of injury, between 24 and 36 h [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

The beneficial effect of cold atmospheric plasma on parameters of molecules and cell function involved in wound healing in human osteoblast-like cells in vitro

et al. 2020

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The aim of this study was to analyse the effect of cold atmospheric plasma (CAP) on human osteoblast-like cells in vitro. Additionally, underlying intracellular mechanisms were to be studied. Human osteoblast-like (MG63) cells were exposed to CAP for 60 s. The effects of CAP on key molecules essential for the wound healing response were studied using real-time PCR, ELISA and immunocytochemistry. For studying intracellular signalling pathways, MAP kinase MEK 1/2 was blocked. Cell viability was analysed by an XTT assay and with an EVE automated cell counter. Cell migration was examined by an in vitro wound healing assay. CAP exposition on osteoblast-like cells caused a significant upregulation of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)α, cyclooxygenase (COX)2, collagen (COL) 1α, matrix metalloproteinase (MMP)1, Ki67, proliferating-cellnuclear-antigen (PCNA) and chemokine ligand (CCL)2 mRNA expression at 1 day. Interestingly, after blocking of MAP kinase, CAP-induced upregulation of Ki67 was inhibited by 57%. Moreover, CAP treatment improved significantly osteoblastlike cell viability as compared to untreated cells at 1 day. Beneficial effect of CAP treatment was shown by an in vitro wound healing assay, displaying a significant faster wound closure. Our findings provide evidence that CAP exposure effects gene and protein regulation in human osteoblast-like cells. Furthermore, CAP treatment has a positive impact on wound closure in an in vitro setting and might improve existing concepts of hard tissue regeneration in the future.

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Recent Advances in Cell Sheet-Based Tissue Engineering for Bone Regeneration

Cao,

Ren,

2024

Tissue Engineering Part B: Reviews

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Alveolar bone healing in mice genetically selected in the maximum (AIRmax) or minimum (AIRmin) inflammatory reaction

Cited by 8 publications

References 81 publications

Macrophage Polarization and Alveolar Bone Healing Outcome: Despite a Significant M2 Polarizing Effect, VIP and PACAP Treatments Present a Minor Impact in Alveolar Bone Healing in Homeostatic Conditions

Macrophage Polarization and Alveolar Bone Healing Outcome: Despite a Significant M2 Polarizing Effect, VIP and PACAP Treatments Present a Minor Impact in Alveolar Bone Healing in Homeostatic Conditions

The beneficial effect of cold atmospheric plasma on parameters of molecules and cell function involved in wound healing in human osteoblast-like cells in vitro

Recent Advances in Cell Sheet-Based Tissue Engineering for Bone Regeneration

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