Aluminum ions alter the function of non-specific phospholipase C through the changes in plasma membrane physical properties

Pejchar, Přemysl; Martinec, Jan

doi:10.1080/15592324.2015.1031938

Cited by 8 publications

(5 citation statements)

References 44 publications

(43 reference statements)

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“…Aluminum also reduced PC-derived DAG production in Arabidopsis and this was rescued by NPC4 overexpression and npc4 was more sensitive to aluminum stress [150]. The aluminum-decreased NPC activity in Arabidopsis was later found to be through aluminum-induced changes in plasma membrane physical properties [151]. NPC activity was down-regulated in parsley cells by a glycoprotein elicitor from Phytophthra sojae and in tobacco cells by the small protein cryptogein from Phytophthra cryptogea [147].…”

Section: Substrate Preference and Regulation Of Npc Activitiesmentioning

confidence: 99%

Plant phospholipases D and C and their diverse functions in stress responses

Hong

Zhao

Guo

et al. 2016

Progress in Lipid Research

294

229

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Phospholipases D (PLD) and C (PLC) hydrolyze the phosphodiesteric linkages of the head group of membrane phospholipids. PLDs and PLCs in plants occur in different forms: the calcium-dependent phospholipid binding domain-containing PLDs (C2-PLDs), the plekstrin homology and phox homology domain-containing PLDs (PX/PH-PLDs), phosphoinositide-specific PLC (PI-PLC), and non-specific PLC (NPC). They differ in structures, substrate selectivities, cofactor requirements, and/or reaction conditions. These enzymes and their reaction products, such as phosphatidic acid (PA), diacylglycerol (DAG), and inositol polyphosphates, play important, multifaceted roles in plant response to abiotic and biotic stresses. Here, we review biochemical properties, cellular effects, and physiological functions of PLDs and PLCs, particularly in the context of their roles in stress response along with advances made on the role of PA and DAG in cell signaling in plants. The mechanism of actions, including those common and distinguishable among different PLDs and PLCs, will also be discussed.

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Section: Substrate Preference and Regulation Of Npc Activitiesmentioning

confidence: 99%

Plant phospholipases D and C and their diverse functions in stress responses

Hong

Zhao

Guo

et al. 2016

Progress in Lipid Research

294

229

View full text Add to dashboard Cite

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“…To date, physiological roles for four of these NPC isoforms have been described, excepting NPC2 and NPC6. NPC4 and NPC5 play a role in response to phosphate starvation (Nakamura et al ., ; Gaude et al ., ) and salt stress (Peters et al ., , ; Kocourkova et al ., ), NPC4 in aluminum toxicity (Pejchar et al ., , ; Pejchar & Martinec, ), NPC3 and NPC4 in brassinolide signaling (Wimalasekera et al ., ), and NPC1 in the heat stress response (Krčková et al ., ). Thus, while the involvement of NPCs in stress responses is well described, it is not known whether NPCs are involved in reproductive development.…”

Section: Introductionmentioning

confidence: 99%

A pair of nonspecific phospholipases C, NPC2 and NPC6, are involved in gametophyte development and glycerolipid metabolism in Arabidopsis

et al. 2018

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Phospholipases play crucial roles in plant membrane lipid homeostasis. Nonspecific phospholipase C (NPCs) establish a unique class of phospholipases found only in plants and certain bacteria. Here, we show that two previously uncharacterized NPC isoforms, NPC2 and NPC6, are required for male and female gametophyte development in Arabidopsis. Double mutant plants of npc2-1 npc6-2 could not be retrieved because npc2-1 npc6-2 ovule and pollen development is affected. Genetic complementation, reciprocal crossing and microscope observation of npc2-1/- npc6-2/+ and npc2-1/+ npc6-2/- plants suggest that NPC2 and NPC6 are redundant and are required for normal gametophyte development. Both NPC2 and NPC6 proteins are localized to the plastids. Promoter-GUS assays in transgenic Arabidopsis revealed that NPC2 and NPC6 are preferentially expressed in floral organs rather than in leaves. In vitro enzyme assays showed that NPC2 and NPC6 hydrolyze phosphatidylcholine and phosphatidylethanolamine, but not phosphatidate, being consistent with the reported substrate selectivity of NPCs. The amounts of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol were increased in buds but not in flowers of npc2-1/- npc6-2/+ and npc2-1/+ npc6-2/- plants, presumably due to reduced phospholipid hydrolysis activity in developing flowers. Our results demonstrate that NPC2 and NPC6 play crucial roles in gametogenesis during flower development.

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“…Aluminum ion (Al) is a major toxic factor in acidic soils that affects crop production. Using tobacco BY-2 cells incubated with fluorescent PC, AlCl 3 treatment reduced content of fluorescent DAG, which was more enriched in plasma membrane fraction than in microsomal fraction (Pejchar and Martinec 2015 ; Pejchar et al 2010 ). GUS reporter assay showed that NPC4-GUS expression was reduced after AlCl 3 treatment in root, and knockout mutant of NPC4 showed higher sensitivity to AlCl 3 -induced stress than wild type (Pejchar et al 2015 ).…”

Section: Emerging Functions Of Npcs In Arabidopsismentioning

confidence: 99%

Non-specific phospholipase C (NPC): an emerging class of phospholipase C in plant growth and development

Nakamura

Ngo

2020

J Plant Res

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Non-specific phospholipase C (NPC) is a novel class of phospholipase C found only in bacteria and higher plants. NPC hydrolyzes major phospholipid classes such as phosphatidylcholine (PC) and phosphatidylethanolamine (PE) to produce diacylglycerol (DAG) and a corresponding phosphate-containing polar head group. Originally known as a toxin in certain bacteria to invade the host cell, this class of phospholipase has been well-investigated in bacteriology. Since the first discovery of eukaryotic NPC in Arabidopsis in 2005, this emerging class of phospholipase has received greater attention in plant biology in elucidating the biochemical characteristics and physiological function in the context of plant growth regulation and stress response. Particularly in the last few years, there has been significant progress made in understanding the fundamental character of 6 NPC isoforms in Arabidopsis, as well as novel function in other plant models. Now that research with plant NPC is entering into a new phase, this review aims to summarize recent progress in plant NPC along with some future perspectives.

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Aluminum ions alter the function of non-specific phospholipase C through the changes in plasma membrane physical properties

Cited by 8 publications

References 44 publications

Plant phospholipases D and C and their diverse functions in stress responses

Plant phospholipases D and C and their diverse functions in stress responses

A pair of nonspecific phospholipases C, NPC2 and NPC6, are involved in gametophyte development and glycerolipid metabolism in Arabidopsis

Non-specific phospholipase C (NPC): an emerging class of phospholipase C in plant growth and development

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