Aluminum induces a stress response in zebrafish gills by influencing metabolic parameters, morphology, and redox homeostasis

Napolitano, Gaetana; Capriello, Teresa; Venditti, Paola; Fasciolo, Gianluca; Pietra, Alessandra La; Trifuoggi, Marco; Giarra, Antonella; Agnisola, Claudio; Ferrandino, Ida

doi:10.1016/j.cbpc.2023.109633

Cited by 5 publications

(3 citation statements)

References 61 publications

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“…Fluorescence was measured in a multimode microplate reader (Synergy™ HTX Multimode Microplate Reader, BioTek, Winooski, VT, United States). Total antioxidant capacity (ABTS) of cells was detected following the decolorization of the radical 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+ ) [ 103 , 104 ]. In brief, nonradical ABTS was incubated overnight with potassium persulfate (245 mM) to obtain the radical ABTS •+ whose decolorization induced by cells’ antioxidants was then determined at 734 nm.…”

Section: Methodsmentioning

confidence: 99%

Effects of Dibutylphthalate and Steroid Hormone Mixture on Human Prostate Cells

Mileo,

Chianese,

Fasciolo

et al. 2023

IJMS

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Phthalates are a family of aromatic chemical compounds mainly used as plasticizers. Among phthalates, di-n-butyl phthalate (DBP) is a low-molecular-weight phthalate used as a component of many cosmetic products, such as nail polish, and other perfumed personal care products. DBP has toxic effects on reproductive health, inducing testicular damage and developmental malformations. Inside the male reproductive system, the prostate gland reacts to both male and female sex steroids. For this reason, it represents an important target of endocrine-disrupting chemicals (EDCs), compounds that are able to affect the estrogen and androgen signaling pathways, thus interfering with prostate homeostasis and inducing several prostate pathologies. The aim of this project was to investigate the effects of DBP, alone and in combination with testosterone (T), 17β-estradiol (E2), and both, on the normal PNT1A human prostate cell-derived cell line, to mimic environmental contamination. We showed that DBP and all of the tested mixtures increase cell viability through activation of both estrogen receptor α (ERα) and androgen receptor (AR). DBP modulated steroid receptor levels in a nonmonotonic way, and differently to endogenous hormones. In addition, DBP translocated ERα to the nucleus over different durations and for a more prolonged time than E2, altering the normal responsiveness of prostate cells. However, DBP alone seemed not to influence AR localization, but AR was continuously and persistently activated when DBP was used in combination. Our results show that DBP alone, and in mixture, alters redox homeostasis in prostate cells, leading to a greater increase in cell oxidative susceptibility. In addition, we also demonstrate that DBP increases the migratory potential of PNT1A cells. In conclusion, our findings demonstrate that DBP, alone and in mixtures with endogenous steroid hormones, acts as an EDC, resulting in an altered prostate cell physiology and making these cells more prone to cancer transformation.

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Section: Methodsmentioning

confidence: 99%

Effects of Dibutylphthalate and Steroid Hormone Mixture on Human Prostate Cells

Mileo,

Chianese,

Fasciolo

et al. 2023

IJMS

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“…Contaminants/heavy metals can have a negative impact on the physiology of aquatic organisms; fish especially are very sensitive to such changes [15]. The toxic effects of heavy metals on fish are numerous [16]. They enter the fish body via the gills, digestive tract, and body surface and accumulate in the liver, kidney, muscle, intestine, skin, and bones [16].…”

Section: Pollution and The Environmentmentioning

confidence: 99%

“…The toxic effects of heavy metals on fish are numerous [16]. They enter the fish body via the gills, digestive tract, and body surface and accumulate in the liver, kidney, muscle, intestine, skin, and bones [16]. It is shown that in fish heavy metals can alter several physiological and biochemical processes, growth rates, mortality, and reproduction.…”

Section: Pollution and The Environmentmentioning

confidence: 99%

The Impact of Micro- and Nanoplastics on Aquatic Organisms: Mechanisms of Oxidative Stress and Implications for Human Health—A Review

Geremia,

Muscari Tomajoli,

Murano

et al. 2023

Environments

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In recent years, interest in the impact of environmental pollutants on the ecosystem has increased significantly, with particular attention being paid to the relationship between climate change and the aquatic world. This is because increasing pollution is causing fundamental changes to the welfare of animals and the marine ecosystem. A primary focus is on the impact of microplastics (MPs) and nanoplastics (NPs), as evidenced by our bibliometric network analysis (BNA). However, while research is focused on the accumulation of these pollutants in aquatic organisms, their effects on redox homeostasis are still seldom discussed despite the role played by reactive oxygen species and mitochondrial well-being in maintaining an optimal state of health. However, some scientific evidence suggests that the accumulation of MPs and NPs in organisms at the base of the trophic chain can cause a transfer of these substances towards more complex organisms, reaching humans through the consumption of aquatic fauna as food. Therefore, in this review, we have tried to discuss the effects of these substances on oxidative stress in aquatic organisms, even if studies in this regard are still scarce.

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