Aluminum-induced secretion of organic acid by cowpea (Vigna unguiculata L.) roots

Li, Yu; Yan, Jun; Guo, Shirong; Zhu, Weimin

doi:10.1016/j.scienta.2011.12.006

Cited by 10 publications

(10 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On drought stress, SDH was found to be upregulated in Ilex paraguariensis leaves 53 . In summer months, SDH activity of cauliflower was observed to be less 54 ; similarly, in cowpea on Al stress it showed decreased activity 40,55 . SDH, frequently denoted to as complex II, has a dual activity; hence plays a substantial part in both the tricarboxylic acid cycle and the aerobic respiratory chain by catalyzing the oxidation of succinate to fumarate, and the reduction of ubiquinone to ubiquinol, respectively.…”

Section: Discussionmentioning

confidence: 88%

Redox balance, metabolic fingerprint and physiological characterization in contrasting North East Indian rice for Aluminum stress tolerance

Awasthi

Saha

Panigrahi

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Aluminum (Al) toxicity is a serious problem for rice crop productivity in acidic soils worldwide. The present work was conducted to look out for the alteration in ROS homeostasis; metabolic fingerprint; and morphology in two contrasting Indica rice cultivars of North East India (NE India) to Al toxicity. Al stress led to excess accumulation of ROS (H 2 O 2 and O 2 − ), and this in turn induced ROS mediated cellular damage, as indicated by lipid peroxidation both qualitatively as well as quantitatively. This excessive ROS production also led to significant reduction in chlorophyll content and stomatal conductance. This was followed by the loss of photosynthetic efficiency as detected by chlorophyll fluorescence. This excessive damage due to ROS prompted us to check the anti-oxidative machinery. Antioxidants, especially enzymes (SOD, APX, POX, GR, CAT, DHAR, MDHAR) are very important players in maintenance of ROS homeostasis. In tolerant variety Disang, higher activity of these enzymes and vice versa in sensitive variety, was observed in response to Al treatment. The non-enzymatic antioxidants (proline, ascorbate and glutathione) also showed similar trend. Though the tolerant variety showed strong anti-oxidative machinery, it was unable to completely nullify the stress experienced by the seedlings. Organic acids are also important players in detoxification of Al stress through efflux in the rhizosphere. In tolerant genotype, citrate exudate was found to be more when compared to sensitive genotypes on exposure to high dose of Al. This is supported by higher abundance of FRDL4, a citrate transporter. Not only FRDL4, other stakeholders for Al stress response like ART1 and ALS1 depicted prominent transcript abundance in the tolerant variety. In conclusion, through this study detailed physiological and metabolic characterisation of two contrasting Indica rice varieties Disang and Joymati, native to NE India for Al tolerance was performed for the very first time.

show abstract

Section: Discussionmentioning

confidence: 88%

Redox balance, metabolic fingerprint and physiological characterization in contrasting North East Indian rice for Aluminum stress tolerance

Awasthi

Saha

Panigrahi

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…On the other hand, both PG and CHM signi cantly reduced the Alinduced secretion and internal concentration of citrate in both Eucalyptus clones roots as well as the malate in G9 roots, but CHM had no impact on malate secretion in W4 root, which may indicate that there is genetic variation in this response within the genus. Generally, Al-tolerant species or varieties had higher activation and quantities of carrier proteins and anion channel proteins than Al-sensitive genotypes (Piñeros et al, 2002;Li et al, 2009;Yang et al, 2011;Yu et al, 2012). Therefore, it may be inferred that the citrate and malate secreted by the Al-tolerant G9 may require both an anion channel and synthesis of new proteins.…”

Section: The Transport Pathways Of Citrate and Malate Secretion Are Pmentioning

confidence: 99%

Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

Finnegan

Dai

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Eucalyptus is the main timber species, most of which are hybrid clones, and usually grow in aluminized acid soil in China. The exudation of organic acids from roots may contribute to detoxification of Al and lead to the Al-tolerance in Eucalyptus genotypes. To further understand the organic acid response in Al tolerance in Eucalyptus, the Al-tolerant Eucalyptus grandis × E. urophylla clone GL-9 (marked as “G9”) and the Al-sensitive Eucalyptus urophylla clone GL-4 (marked as “W4”) were used to investigate the secretion and metabolism of citrate and malate in roots. Results Eucalyptus seedlings in hydroponics were exposed to the presence or absence of 4.4 mM Al at pH 4.0 for 24 hours. The protein synthesis inhibitor cycloheximide (CHM) and the anion channel blocker phenylglyoxal (PG) were applied to explore possible pathways involved in organic acid secretion. The Al treatments caused higher Al accumulation in roots of both clones. The secretion of malate and citrate was greater in G9 than in W4, corresponding to the relatively higher tolerance in G9 to Al. The peak Al concentration occurred after 1 h in G9 roots and declined afterward, indicating the activation of detoxification to alleviate Al accumulation. After 6 h of Al exposure, the efflux of citrate dramatically increased in G9 after a substantial lag phase, while both peak Al accumulation in roots and peak malate secretion occurred and there was no induction of citrate secretion in W4. Enhanced activity for citrate synthase and phosphoenolpyruvate carboxylase, and reduced activity for NADP-isocitrate dehydrogenase, aconitase and NADP-malic enzyme were closely associated with the greater secretion of citrate in G9. Both PG and CHM were effective inhibitors of citrate and malate secretion in both Eucalyptus clones, except the malate secretion in W4 was not affected by CHM. Conclusions In two different Al-tolerant Eucalyptus clones, both secretion and internal accumulation of citrate and malate in roots were involved in Al detoxification. An anion channel on the plasma membrane could be an important mode of organic acid secretion. Citrate and relevant metabolizing enzymes led more important role in the response to Al in E. grandis × E. urophylla.

show abstract

“…However, CHM had no impact on malate secretion in W4, indicating that there are different pathways operating for citrate and malate secretion in response to Al in the two clones. Generally, Al-tolerant species or genotypes had stronger induction and higher quantities of carrier proteins on membranes inside root cells and anion channel proteins on the plasma membrane of root cells, than Al-sensitive genotypes [37,38]. If organic acid synthesis and transport need new carrier proteins involved, an obvious lag of several hours before secretion would be apparent ; an anion channel proteins already present in allow the organic acid to be secreted out of the root more quickly [39,40].…”

Section: An Al-tolerant Eucalyptus Hybrid Clone Has Enhanced Accumulamentioning

confidence: 99%

“…After plants were cultured for 24 h in the presence or absence of Al, the activities of PEPC, MDH, CS, NADP-dependent IDH, NADP-dependent NADP-ME and ACO were measured. Based on the methods of Chen et al [59] and Yu et al [38], 200 mg of fresh root apices were homogenized in ice-cold extraction buffer containing 50 mM HEPES-NaOH, pH 7.5, 5 mM MgCl 2 , 5 mM EDTA, 10% (…”

Section: Activity Measurement Of Organic Acid-metabolizing Enzymesmentioning

confidence: 99%

Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

Finnegan

Dai

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Eucalyptus is the main plantation wood species, mostly grown in aluminized acid soils. To understand the response of Eucalyptus clones to aluminum (Al) toxicity, the Al-tolerant Eucalyptus grandis × E. urophylla clone GL-9 (designated “G9”) and the Al-sensitive E. urophylla clone GL-4 (designated “W4”) were employed to investigate the production and secretion of citrate and malate by roots.Results: Eucalyptus seedlings in hydroponics were exposed to the presence or absence of 4.4 mM Al at pH 4.0 for 24 hours. The protein synthesis inhibitor cycloheximide (CHM) and anion channel blocker phenylglyoxal (PG) were applied to explore possible pathways involved in organic acid secretion. The secretion of malate and citrate was earlier and greater in G9 than in W4, corresponding to less Al accumulation in G9. The concentration of Al in G9 roots peaked after 1h and decreased afterwards, corresponding with a rapid induction of malate secretion. A time-lag of about 6h in citrate efflux in G9 was followed by robust secretion to support continuous Al-detoxification. Malate secretion alone may alleviate Al toxicity because the peaks of Al accumulation and malate secretion were simultaneous in W4, which did not secrete appreciable citrate. Enhanced activities of citrate synthase (CS) and phosphoenolpyruvate carboxylase (PEPC), and reduced activities of isocitrate dehydrogenase (IDH), aconitase (ACO) and malic enzyme (ME) were closely associated with the greater secretion of citrate in G9. PG effectively inhibited citrate and malate secretion in both Eucalyptus clones. CHM also inhibited malate and citrate secretion in G9, and citrate secretion in W4, but notably did not affect malate secretion in W4.Conclusions: G9 immediately secrete malate from roots, which had an initial effect on Al-detoxification, followed by time-delayed citrate secretion. Pre-existing anion channel protein first contributed to malate secretion, while synthesis of carrier protein appeared to be needed for citrate excretion. The changes of organic acid concentrations in response to Al can be achieved by enhanced CS and PEPC activities, but was supported by changes in the activities of other enzymes involved in organic acid metabolism. The above information may help to further explore genes related to Al-tolerance in Eucalyptus.

show abstract

Aluminum-induced secretion of organic acid by cowpea (Vigna unguiculata L.) roots

Cited by 10 publications

References 37 publications

Redox balance, metabolic fingerprint and physiological characterization in contrasting North East Indian rice for Aluminum stress tolerance

Redox balance, metabolic fingerprint and physiological characterization in contrasting North East Indian rice for Aluminum stress tolerance

Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

Metabolic acclimation supports higher aluminium-induced secretion of citrate and malate in an aluminium-tolerant hybrid clone of Eucalyptus

Contact Info

Product

Resources

About