Aluminium-induced reduction of plant growth in alfalfa (Medicago sativa) is mediated by interrupting auxin transport and accumulation in roots

Wang, Shengyin; Ren, Xiaoyan; Huang, Bingru; Wang, Ge; Zhou, Peng; An, Yuan

doi:10.1038/srep30079

Cited by 63 publications

(54 citation statements)

References 56 publications

(76 reference statements)

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“…Soil contains various toxic and non‐essential heavy metal elements including Cr, Cd, and Al, which affect various metabolic, developmental, and physiological processes in plants, leading to a reduction in leaf area, primary root elongation, and lateral and adventitious root development (Eleftheriou et al, ; López‐Bucio et al, ; Sun et al, ; Wang et al, ; Yuan & Huang, ). Cr stress also interferes with the uptake of essential elements, reduces germination rate, detriments the cellular ultrastructures, and represses mitotic cell division and cell elongation (Eleftheriou et al, ; Ortiz‐Castro et al, ; Shanker et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Ethylene and auxin act synergistically to regulate root growth and development (Guo, Kong, & Yang, ; Marciniak, Glazinska, Kesy, & Tretyn, ; Muday et al, ), and inhibit primary root elongation in response to other exogenous stimuli, such as aluminum and alkaline stresses (Li, Xu, Liu, et al, ; Sun et al, ; Wang et al, ). The probability that ethylene and auxin participate collectively in plant responses to Cr(VI) was investigated in our study, which confirmed the crosstalk between ethylene and auxin.…”

Section: Discussionmentioning

confidence: 99%

“…Asterisks show a significant difference on the basis of two-way ANOVA with Tukey's HSD Test: ***, p < .001. ACC = 1-aminocyclopropane-1-carboxylic acid; CR(VI) = hexavalent; WT = wild-type [Colour figure can be viewed at wileyonlinelibrary.com] developmental, and physiological processes in plants, leading to a reduction in leaf area, primary root elongation, and lateral and adventitious root development (Eleftheriou et al, 2015;López-Bucio et al, 2015;Sun et al, 2010;Wang et al, 2016;Yuan & Huang, 2016). Cr stress also interferes with the uptake of essential elements, reduces germination rate, detriments the cellular ultrastructures, and represses mitotic cell division and cell elongation (Eleftheriou et al, 2015;Ortiz-Castro et al, 2007;Shanker et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

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Ethylene mediates dichromate‐induced inhibition of primary root growth by altering AUX1 expression and auxin accumulation in Arabidopsis thaliana

Wakeel

Ali

Upreti

et al. 2018

Plant Cell & Environment

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The hexavalent form of chromium [Cr(VI)] causes a major reduction in yield and quality of crops worldwide. The root is the first plant organ that interacts with Cr(VI) toxicity, which inhibits primary root elongation, but the underlying mechanisms of this inhibition remain elusive. In this study, we investigate the possibility that Cr(VI) reduces primary root growth of Arabidopsis by modulating the cell cycle-related genes and that ethylene signalling contributes to this process. We show that Cr(VI)-mediated inhibition of primary root elongation was alleviated by the ethylene perception and biosynthesis antagonists silver and cobalt, respectively. Furthermore, the ethylene signalling defective mutants (ein2-1 and etr1-3) were insensitive, whereas the overproducer mutant (eto1-1) was hypersensitive to Cr(VI). We also report that high levels of Cr(VI) significantly induce the distribution and accumulation of auxin in the primary root tips, but this increase was significantly suppressed in seedlings exposed to silver or cobalt. In addition, genetic and physiological investigations show that AUXIN-RESISTANT1 (AUX1) participates in Cr(VI)-induced inhibition of primary root growth. Taken together, our results indicate that ethylene mediates Cr(VI)-induced inhibition of primary root elongation by increasing auxin accumulation and polar transport by stimulating the expression of AUX1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Ethylene mediates dichromate‐induced inhibition of primary root growth by altering AUX1 expression and auxin accumulation in Arabidopsis thaliana

Wakeel

Ali

Upreti

et al. 2018

Plant Cell & Environment

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“…Alfalfa (WL-525), which is an Al-tolerant cultivar [24, 25], was obtained from the National Seed Corporation (New Delhi, India). Healthy seeds of uniform size were surface-sterilized with 0.5% (v/v) sodium hypochlorite solution and repeatedly washed with double-distilled water.…”

Section: Methodsmentioning

confidence: 99%

Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum

Zhou

et al. 2016

International Journal of Genomics

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Acid-Aluminum (Al) is toxic to plants and greatly affects crop production worldwide. To understand the responses of plants to acid soils and Aluminum toxicity, we examined global gene expression using microarray data in alfalfa seedlings with the treatment of acid-Aluminum. 3,926 genes that were identified significantly up- or downregulated in response to Al3+ ions with pH 4.5 treatment, 66.33% of which were found in roots. Their functional categories were mainly involved with phytohormone regulation, reactive oxygen species, and transporters. Both gene ontology (GO) enrichment and KEGG analysis indicated that phenylpropanoid biosynthesis, phenylalanine metabolism, and flavonoid biosynthesis played a critical role on defense to Aluminum stress in alfalfa. In addition, we found that transcription factors such as the MYB and WRKY family proteins may be also involved in the regulation of reactive oxygen species reactions and flavonoid biosynthesis. Thus, the finding of global gene expression profile provided insights into the mechanisms of plant defense to acid-Al stress in alfalfa. Understanding the key regulatory genes and pathways would be advantageous for improving crop production not only in alfalfa but also in other crops under acid-Aluminum stress.

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“…In soils with 0.7 cmol/kg Al 3+ , root weight reduced by 35% to 73% (differed among cultivars) compared to those in soils with 0.02 cmol/kg Al 3+ (Khu et al, 2012). Root weight of alfalfa exposed to 100 μM AlCl 3 reduced by 56.1% than those in 0 μM AlCl 3 (Wang, Ren, Huang, Wang, Zhou, & An, 2016). In solutions with 2 mM AlCl 3 and 4 mM AlCl 3 , 12 from 13 alfalfa cultivars showed significant inhibition of biomass accumulation when compared to that in 0 mM AlCl 3 (Pan et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

The Efficiency of Arbuscular Mycorrhizal Fungi in Promoting Alfalfa Growth in Acid Soils

Huang¹,

He²,

Guo³

2017

JAS

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High concentrations of soil Al 3+ in acid soil severely influence the growth of Medicago sativa (alfalfa). The objective of the current study was to analyze whether Arbuscular Mycorrhizal Fungi (AMF) inoculation could improve alfalfa growth in acid soils. A two-way completely randomized factorial design was employed for M. sativa and M. lupulina (black medick) with two inoculations (rhizobia and AMF) and three Al 3+ levels, and replicated four times. The soil Al 3+ levels were adjusted to 900 mg/kg, 1000 mg/kg and 1100 mg/kg. Spores of AMF were isolated directly from rhizosphere soils of black medick. The rhizobia were isolated from root nodules in fields separately from two plant species. At each Al 3+ level, there were four inoculations, non-inoculation, AMF solely, rhizobia solely and dual-inoculation with AMF and rhizobia. Soil Al 3+ concentration significantly limited above-and below-ground growth of both alfalfa and black medick, reducing plant height, branching number, shoot and root weight, and root length, surface area and volume. Compared to rhizobia, AMF showed a higher tolerance to soil Al 3+ . AMF inoculation increased the shoot and root weight of both plant species under most circumstances. Overall, AMF colonization had a trend in increasing the contents of phosphorus in both plant species at all Al 3+ concentrations but not nitrogen and potassium. Dual inoculation significantly increased nodulation ability, enabling both plant species to form nodules at 900 and 1000 mg/kg Al 3+ . Though the soil Al 3+ concentration influenced the efficiency of AMF inoculation, AMF inoculation improved nodulation, increased plant growth and nutrient uptake, suggesting that it was an alternative way in improving alfalfa growth in acid soils.

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Aluminium-induced reduction of plant growth in alfalfa (Medicago sativa) is mediated by interrupting auxin transport and accumulation in roots

Cited by 63 publications

References 56 publications

Ethylene mediates dichromate‐induced inhibition of primary root growth by altering AUX1 expression and auxin accumulation in Arabidopsis thaliana

Ethylene mediates dichromate‐induced inhibition of primary root growth by altering AUX1 expression and auxin accumulation in Arabidopsis thaliana

Gene Expression Analysis of Alfalfa Seedlings Response to Acid-Aluminum

The Efficiency of Arbuscular Mycorrhizal Fungi in Promoting Alfalfa Growth in Acid Soils

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