Aluminium-induced production of oxygen radicals, lipid peroxidation and DNA damage in seedlings of rice (Oryza sativa)

Meriga, Balaji; Reddy, B. Krishna; Rao, K. R.; Reddy, L. Ananda; Kishor, P. B. Kavi

doi:10.1078/0176-1617-01156

Cited by 177 publications

(77 citation statements)

References 32 publications

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“…Lee et al (2001) suggested that, enhanced activity of superoxide dismutase may function in signaling of oxidative stress and hence, leads to the induction of antioxidant enzymes associated with an H 2 O 2 scavenging system, particularly an ascorbateglutathione cycle. Al-induced high activity of SOD that was observed in the present study is consistent with previous results obtained from different plants (Boscolo et al, 2003;Tamas et al, 2003;Meriga et al, 2004;Sharma and Dubey, 2007). The marked increase of catalase activity with increasing Al levels in Maroon and Darab varieties may indicate enhanced production of reactive oxygen species (ROS) under an excess of aluminium (Al).…”

Section: Discussionsupporting

confidence: 82%

The effect of aluminium on enzyme activities in two wheat cultivars

Nasr¹,

Carapetian²,

Heidari³

et al. 2011

Afr. J. Biotechnol.

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In order to study the effect of different aluminum (Al) concentrations on the enzyme activities of wheat seedlings and the effect of malate and citrate treatments as chelates for reducing the noxious effect of Al in medium culture, the seedlings of two wheat cultivars, Darab (Al-sensitive) and Maroon (Al-tolerant) were grown on hydroponic solution (non modified Hoagland solution) containing AlCl 3 (0-100-200-300 M). Factorial experiment was realized in a complete randomized design with three replications. The activity of different enzymes such as ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR) and superoxide dismutase (SOD) in root and shoot were measured. Analysis of variance revealed that, the activity of studied enzymes (APX, CAT, GR and SOD) in root and shoot were affected by the main effects of Al concentration. However, in the case of catalase activity in root, the main effect of genotypes as well as genotype× × × ×Al concentration was also significant. APX activity in root was not significantly differed between Maroon and Darab in all Al concentrations. But by increasing Al concentration in root medium APX activity was significantly decreased. In the case of SOD activity, we did not find any difference between the studied genotypes in all Al concentrations but its content in roots was affected by the amount of Al applied in medium. So that by increasing the amount of Al, SOD content increased in the genotypes similarly. The same trend was observed for catalase activity in root. In the case of GR activity, we did not find any difference between the genotypes in all Al concentrations but its content in root was affected by applying Al in medium compared with control medium, so that GR content increased in both genotypes similarly. The activity of investigated enzymes showed the same trend in the shoot. The effect of malate and citrate was also studied on reducing the noxious effect of Al in root. Analysis of variance revealed that, there were significant differences within the treatments on the enzymes activity in root (not in the shoot) except for catalase. However, some interaction effects were significant. This means that malate or citrate application was effective in some Al concentrations.

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Section: Discussionsupporting

confidence: 82%

The effect of aluminium on enzyme activities in two wheat cultivars

Nasr¹,

Carapetian²,

Heidari³

et al. 2011

Afr. J. Biotechnol.

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“…DNA damage is a well known phenomenon of Al toxicity [46][47][48]. Matsumoto [49] proposed that DNA-Al complex formation and repression of transcription by Al could be a …”

Section: Nucleotide Synthesis and Related Carbon Pathways Affected Bymentioning

confidence: 99%

Metabolic alterations proposed by proteome in rice roots grown under low P and high Al concentration under low pH

et al. 2007

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Growth inhibition caused by acid soils, especially due to P deficiency and Al stress, is a serious problem for crop production. To comprehend the adaptation mechanisms of rice plants to P deficiency and Al stress conditions, a proteomic analysis of rice roots in hydroponic cultivation was demonstrated. 464 detectable proteins spots were separated by 2D-PAGE. 56 of 94 spots selected at random were identified by peptide mass fingerprinting. In general, the proteomic alterations under P deficiency and Al stress conditions were similar trend, indicating that a common metabolic system is responsive to both P deficiency and Al stress. An increase in nucleotide monomer synthesis was indicated from the related proteomic alterations, which mediate the reversible reactions of the triose phosphate/pentose phosphate pool, and the oxidative reactions of the pentose phosphate pathway under both stress conditions. Carbon flow to the TCA cycle and N assimilation were altered in proteomic level. The changes could be contributed to the complementation of TCA components from suppression of photosynthates partitioning from leaves, and partly contribute to organic acid secretion. Induction of S-adenosylmethionine (SAM) synthetase is a significant and unique response to Al stress, suggesting that SAM is related to ethylene-mediated inhibition of root growth and/ or the alteration of cell wall structures and polymers in roots.Abbreviations: ACC, 1-aminocyclopropane-1-carboxylic acid; ADK, adenosine kinase; 2D-PAGE, 2 dimensional polyacrylamide gel electrophoresis; DTT, dithiothreitol; PEPC, phosphoenolpyruvate carboxylase; Pi, inorganic phosphate; PMF, peptide mass fingerprinting; SAH, S-adenosyl-L-homocysteine; SAM, S-adenosylmethionine.

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“…These active Al species, primarily Al 3+ , can cause root damage and root growth inhibition by targeting the cell wall , the cytoskeleton , callose production (Sivaguru et al, 2000), DNA (Yamaguchi et al, 1999;Meriga et al, 2004), and the plasma membrane (Elstner et al, 1988) and disturbing stress-signaling pathways including the regulation of cytosolic free Ca 2+ activities (Ramos-Díaz et al, 2007). Consequently, the inhibition of root growth and function on acid soils results in the severe impairment of water and nutrient acquisition by roots, leading to a significant reduction in crop yields (Ryan et al, 2001;Kochian et al, 2004).…”

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confidence: 99%

Low pH, Aluminum, and Phosphorus Coordinately Regulate Malate Exudation through GmALMT1 to Improve Soybean Adaptation to Acid Soils

et al. 2013

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Low pH, aluminum (Al) toxicity, and low phosphorus (P) often coexist and are heterogeneously distributed in acid soils. To date, the underlying mechanisms of crop adaptation to these multiple factors on acid soils remain poorly understood. In this study, we found that P addition to acid soils could stimulate Al tolerance, especially for the P-efficient genotype HN89. Subsequent hydroponic studies demonstrated that solution pH, Al, and P levels coordinately altered soybean (Glycine max) root growth and malate exudation. Interestingly, HN89 released more malate under conditions mimicking acid soils (low pH, +P, and +Al), suggesting that root malate exudation might be critical for soybean adaptation to both Al toxicity and P deficiency on acid soils. GmALMT1, a soybean malate transporter gene, was cloned from the Al-treated root tips of HN89. Like root malate exudation, GmALMT1 expression was also pH dependent, being suppressed by low pH but enhanced by Al plus P addition in roots of HN89. Quantitative real-time PCR, transient expression of a GmALMT1-yellow fluorescent protein chimera in Arabidopsis protoplasts, and electrophysiological analysis of Xenopus laevis oocytes expressing GmALMT1 demonstrated that GmALMT1 encodes a root cell plasma membrane transporter that mediates malate efflux in an extracellular pH-dependent and Al-independent manner. Overexpression of GmALMT1 in transgenic Arabidopsis, as well as overexpression and knockdown of GmALMT1 in transgenic soybean hairy roots, indicated that GmALMT1-mediated root malate efflux does underlie soybean Al tolerance. Taken together, our results suggest that malate exudation is an important component of soybean adaptation to acid soils and is coordinately regulated by three factors, pH, Al, and P, through the regulation of GmALMT1 expression and GmALMT1 function.

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Aluminium-induced production of oxygen radicals, lipid peroxidation and DNA damage in seedlings of rice (Oryza sativa)

Cited by 177 publications

References 32 publications

The effect of aluminium on enzyme activities in two wheat cultivars

The effect of aluminium on enzyme activities in two wheat cultivars

Metabolic alterations proposed by proteome in rice roots grown under low P and high Al concentration under low pH

Low pH, Aluminum, and Phosphorus Coordinately Regulate Malate Exudation through GmALMT1 to Improve Soybean Adaptation to Acid Soils

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