Aluminium differentially modifies lipid metabolism from the phosphoinositide pathway inCoffea arabica cells

A Higher Plant Δ8 Sphingolipid Desaturase with a Preference for (Z)-Isomer Formation Confers Aluminum Tolerance to Yeast and Plants

“…Many of these symptoms occur rapidly and some workers have concluded that aluminum toxicity is initiated by interactions occurring in the extracellular compartment (Horst, 1995) and cell membranes. Aluminum accumulates rapidly in the highly charged cell wall and near the fixed charges and polar groups on the plasma membrane surface, which can displace calcium from critical sites in the apoplasm, alter physical properties of the plasma membrane, change membrane lipid composition, block ion channels, and disrupt signal transduction processes by interfering with phospholipase C metabolism (Haug and Caldwell, 1985;Rengel, 1992;Shi and Haug, 1992;Kinraide et al, 1994;Ryan et al, 1994;Jones and Kochian, 1995;Piñ a-Chable and Hernández-Sotomayor, 2001;Mantinez-Estévez et al, 2003;Stival da Silva et al, 2006). Whereas it remains unclear which, if any, of these reactions are primary causes for aluminum toxicity in plants, it is plausible that aluminum-dependent changes in cell membrane structure and function contribute to the overall stress encountered in acid soils.…”

Does Aluminum Generate a Bonafide Phospholipd Signal Cascade?

“…Previously, it has been shown that Al 3+ did not inhibit the enzyme that catalyzes the phosphorylation of PA to produce DGPP. 8 In summary our data show that Al can modulate the levels of phopholipids either in the presence or absence of a stimulus, perhaps generating per se a phospholipid signal transduction cascade. Also we have provided evidence that Al can affect this signal cascade (Fig.…”

“…10 In addition, PIP 2 have a crucial function in several processes of cellular signaling. 11 It has been reported previously 8 that the incubation of Coffea arabica suspension cells with AlCl 3 stimulates the activity of the phosphoinositide kinases (PIKs) quantified in vitro.…”

“…[4][5][6] Plants showing symptoms of aluminum toxicity are more sensitive to the changes in the environmental conditions, 6 and this can be caused by aluminum-mediated effects on the signal transduction cascades. [7][8][9] One of the main components of the phospholipid signaling are the polyphosphoinositides (PPI), which are involved in several processes of cellular signalling caused by different types of stress, such are saline stress, which causes a fast increase in the phosphatidylinositol 4,5-bisphosphate (PIP 2 ) levels. 10 In addition, PIP 2 have a crucial function in several processes of cellular signaling.…”

Coffee biotechnology

“…These results suggested that in vivo treatment of Coffea cells with AlCl 3 affects the activity of some protein kinases. The effect of Al on phosphoinositide-specific phospholipase C (PLC) and lipid kinase activities also was examined (Martínez-Estévez et al, 2003). Two main effects were seen when cells were treated with AlCl 3 .…”