Alumina particles influence the interactions of cocultured osteoblasts and macrophages

Rodrigo, Ana Moreno; Vallés, Gema; Saldaña, Laura; Rodríguez, M.; Martı́nez, Mauricio; Munuera, L.; Vilaboa, Nuria

doi:10.1002/jor.20007

Cited by 30 publications

(24 citation statements)

References 39 publications

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“…Co-cultures of differentiated THP-1 cells and human primary osteoblasts, in the absence of particles, decreased the levels of secreted TNF-a or IL-1b by THP-1 cells. The influence of osteoblasts to modulate TNF-a secretion by macrophages has been previously detected in other coculture models [42,44] and in this work we have detected that IL-1b secretion is also regulated. Particles modulate the secretion of cytokines typically involved in bone turnover in co-cultures of osteoblasts and macrophages [42][43][44][45].…”

Section: Article In Presssupporting

confidence: 57%

“…Co-cultures of osteoblasts and macrophages have proven to be a valuable tool for studying interactions between these types of cells in the presence of wear particles [42][43][44][45]. Co-cultures of differentiated THP-1 cells and human primary osteoblasts, in the absence of particles, decreased the levels of secreted TNF-a or IL-1b by THP-1 cells.…”

Section: Article In Pressmentioning

confidence: 97%

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Differential inflammatory macrophage response to rutile and titanium particles

et al. 2006

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Section: Article In Presssupporting

confidence: 57%

Section: Article In Pressmentioning

confidence: 97%

Differential inflammatory macrophage response to rutile and titanium particles

et al. 2006

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“…These in vitro results corroborate previous histological findings obtained after intramuscular implantation of BCP microparticles 14. At the same time, BCP microparticles of <20 μm increased production of TNF‐α and, above all, the secretion of IL‐6 by J774 cells confirming other studies,19–21 whereas larger sizes did not (Fig. 4).…”

Section: Discussionsupporting

confidence: 91%

Macrophage and osteoblast responses to biphasic calcium phosphate microparticles

Fellah¹,

Delorme²,

Sohier³

et al. 2009

J Biomedical Materials Res

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The aim of this work was to investigate in vitro the biological events leading to ectopic bone formation in contact with microporous biphasic calcium phosphate (BCP) ceramics. After implantation, microparticles may arise from their degradation and induce an inflammatory response involving macrophages. The secretion of pro-inflammatory cytokines may affect the differentiation of osteoblasts. Mouse macrophage-like (J774) and osteoblast-like (MC3T3-E1) cells were cultured in the presence of BCP microparticles of different sizes (<20, 40-80, or 80-200 microm). The smallest microparticles decreased the viability of both cell types as measured with LDH and methyl tetrazolium salt assays, and enhanced the secretion of pro-inflammatory cytokines (IL-6 and TNF-alpha) by macrophages after 24 h, as revealed by ELISA. Osteoblastic cells were then cultured for 96 h in the presence of these pro-inflammatory cytokines and their differentiation studied by RT-PCR. MC3T3-E1 cells cultured with TNF-alpha showed a decrease in osterix, PTH receptor (PTHR1), and osteocalcin gene expression. On the contrary, IL-6 enhanced the expression of osterix, Runx2, alkaline phosphatase, and osteocalcin compared with plastic. In conclusion, this study shows that the inflammatory response initiated by BCP microparticles may have both detrimental and beneficial effects on osteogenesis.

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“…Aluminum oxide NP, which have good dielectric and abrasive properties, have been studied as potential orthopedic implants and may be more e ective with fewer adverse a ects than conventional materials of larger size on bone structure (Gutwein and Webster, 2004). Alumina particles have caused the release of in ammatory mediators in co-cultures of mouse macrophages and primary cultured human osteoblasts (Rodrigo et al, 2006). Endothelial cells exposed to alumina nanoparticles exhibit an increase in mRNA and protein expression of VCAM-1, ICAM-1, and ELAM-1 as well as an increase in adhesion to monocytes (Oesterling et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Interactions of aluminum nanoparticles with human epidermal keratinocytes

Monteiro‐Riviere

Oldenburg

Inman

2009

J of Applied Toxicology

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Aluminum nanoparticles (Al NP) have been used in applications as diverse as drug delivery, material surface coatings and an ingredient for solid rocket fuel in military explosives and artillery. Although Al NP are used in many civilian and military applications, the health and safety implications of these nanosize particles are not known. To understand the interactions and biological activity of Al NP in human cells, cultured human neonatal epidermal keratinocytes (HEK) were exposed for 24 h to 50 and 80 nm Al NP in concentrations from 4.0 to 0.0004 mg ml(-1) to assess the cytotoxicity and inflammatory potential. UV-Vis measurements and nanoparticle controls revealed that the Al NP interact with the assay dyes. Viability did not decrease in HEK exposed to both the 50 and the 80 nm Al NP at all treatment concentrations with MTT, CellTiter 96 AQueous One (96 AQ) and alamar Blue (aB) viability assays. The 96 AQ and aB assays interact with the Al NP less than MTT, and proved to be the best assays to use with these Al NP. TEM depicted Al NP localized within the cytoplasmic vacuoles of the cells. Cytokine data was variable, indicating possible nanoparticle interactions with the cytokine assays. These studies illustrate the difficulties involved in assessing the biological safety of nanomaterials such as Al NP due to media- and temperature-dependent particle agglomeration and nanoparticle interactions with biomarkers of cytotoxicity.

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Alumina particles influence the interactions of cocultured osteoblasts and macrophages

Cited by 30 publications

References 39 publications

Differential inflammatory macrophage response to rutile and titanium particles

Differential inflammatory macrophage response to rutile and titanium particles

Macrophage and osteoblast responses to biphasic calcium phosphate microparticles

Interactions of aluminum nanoparticles with human epidermal keratinocytes

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