Alternatives for the treatment of infections caused by ESKAPE pathogens

Rosa, Taciéli Fagundes da; Coelho, Silvana Silveira; Foletto, Vitória Segabinazzi; Bottega, Angelita; Serafin, Marissa Bolson; Machado, Catrine de Souza; Franco, Laísa Nunes; Paula, Bruno Rafael de; Hörner, Rosmari

doi:10.1111/jcpt.13149

Cited by 19 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…and potential threat as a nosocomial infection. 28 In this study, we found that 19.6% samples from suspected UTI and RTI infections are positive for K pneumoniae pathogen, which can lead the patients to complicated infections in case of drug resistance. In a similar study, Younis 29 reported 73.3% K pneumoniae prevalence in clinically diseased chicken, which is higher than our study, whereas Martin et al 30 and Zhang et al 31 reported 23% and 73.9%, respectively, prevalence of K pneumoniae in clinical samples.…”

Section: Discussionmentioning

confidence: 74%

Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

Fatima

Liaqat

Akbar

et al. 2021

International Wound Journal

View full text Add to dashboard Cite

Klebsiella pneumoniae is an important pathogen causing hospital-acquired infections in human beings. Samples from suspected patients of K pneumoniae associated with respiratory and urinary tract infections were collected at Bolan Medical Complex, Quetta, Balochistan. Clinical samples (n = 107) of urine and sputum were collected and processed for K pneumoniae isolation using selective culture media. Initially, 30 of 107 isolates resembling Klebsiella spp. were processed for biochemical profiling and molecular detection using gyrase A (gyrA) gene for conformation. The K pneumoniae isolates were analysed for the presence of drug resistance and virulence genes in their genomes. The 21 of 107 (19.6%) isolates were finally confirmed as K pneumoniae pathogens. An antibiogram study conducted against 17 different antibiotics showed that a majority of the isolates are multidrug resistant. All the isolates (100%) were resistant to amoxicillin, cefixime, amoxicillin-clavulanic acid, cefotaxime, and ceftriaxone followed by tetracycline (95.2%), ciprofloxacin and gentamicin (76.2%), sulphamethoxazol (66.7%), nalidixic acid (61.9%), norfloxacine (42.9%), piperacillin-tazobactam (23.8%), cefoperazone-sulbactam (19%), and cefotaxime-clavulanic acid (33.3%), whereas all the isolates showed sensitivity to amikacin, chloramphenicol, and imipenem. The presence of tetracycline, sulphamethoxazol-resistant genes, and extended-spectrum beta-lactamase was reconfirmed using different specific genes. The presence of virulence genes fimH1 and EntB responsible for adherence and enterobactin production was confirmed in the isolates. The high virulence and drug resistance potential of these Klebsiella isolates are of high public health concern. Multidrug resistance and virulence potential in K. pneumoniae are converting these nosocomial pathogens into superbugs and making its management harder.Sareeen Fatima and Faiza Liaqat contributed equally to the scientific work.

show abstract

Section: Discussionmentioning

confidence: 74%

Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

Fatima

Liaqat

Akbar

et al. 2021

International Wound Journal

View full text Add to dashboard Cite

show abstract

“…Lastly, statistical significance was found regarding the capacity of ESKAPE pathogens to be more prone to developing antibiotic resistance than non-ESKAPE pathogens, confirming the need for updating the clinician's armamentarium in critical cases with such high mortality, such as patients with major burns. The two-tailed Fisher's test P value was 0.0483 and the two-tailed Chi-squared P value was 0.0372 (P < 0.05) [43,44].…”

Section: Bacteria Resistance Phenotypesmentioning

confidence: 93%

Antimicrobial Resistance of Eskape Pathogens in Major Burns Patients – One-Year Retrospective Study

HODEA¹

2023

FARMACIA

View full text Add to dashboard Cite

Infections in patients with major burns are among the most frequent causes of complications and an increase in mortality. In particular, a group of bacteria abbreviated ESKAPE, consisting of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, has increasingly become problematic in recent times due to their fast capacity to develop antibiotic resistance. The aim of this study was to analyse ESKAPE pathogens from the Major Burn Units in Clinical Emergency Hospital Bucharest through a retrospective crosssectional study, given the high mortality rate of these patients. Our findings revealed that a large percentage (75%) of patients presented with an ESKAPE pathogen during either direct admission or transfer from another healthcare unit; however, this did not influence mortality. The most frequent isolate was Staphylococcus aureus; however, Acinetobacter baumannii had the highest tendency to develop multidrug or extensive-drug resistance. In particular, ESKAPE bacteria tended to exhibit higher antibiotic resistance properties, as there were more ESKAPE MDR and XDR variants than non-ESKAPE strains. RezumatInfecțiile au fost printre cele mai frecvente cauze ale complicațiilor și mortalității crescute în cazul pacienților cu arsuri grave. În particular, un grup de bacterii abreviat ESKAPE, din care fac parte Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa și speciile genului Enterobacter, a devenit din ce în ce mai problematic în zilele noastre, din cauza capacității lor rapide de a dezvolta rezistență la antibiotice. Scopul acestei lucrări este de a analiza agenții patogeni ESKAPE din Centrul de Arși Grav al Spitalului Clinic de Urgență București, realizând un studiu retrospectiv de tip "cross-sectional" având în vedere mortalitatea ridicată a acestor pacienți. În urma acestui studiu, rezultatele arată că o mare parte dintre pacienți (75%) prezintă un agent patogen ESKAPE fie la admisia directă în spital, fie la admisia prin transfer dintr-o altă unitate spitalicească, fără a influența mortalitatea. Cea mai frecvent izolată bacterie a fost Staphylococcus aureus, în timp ce Acinetobacter baumanii a prezentat cea mai mare tendință de a dezvolta rezistență antibiotică de tip "multi-drug" sau "extensive-drug". O descoperire particulară a fost că bacteriile ESKAPE tind să prezinte proprietăți de rezistență antibiotică mai dezvoltate, întrucât au fost mai multe variante MDR și XDR ESKAPE, comparativ cu bacteriile non-ESKAPE.

show abstract

“…Being very common in hospitals, prisons, and nursing homes (where immunocompromised patients and people with open wounds and/or invasive devices such as catheters are at greater risk of hospital-acquired infections), MRSA represents a global health threat and a clear ‘One Health’ problem. Moreover, MRA can spread and impact the environment, animals, and several human sectors [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

Anti Gram-Positive Bacteria Activity of Synthetic Quaternary Ammonium Lipid and Its Precursor Phosphonium Salt

Bacchetti,

Schito,

Milanese

et al. 2024

IJMS

View full text Add to dashboard Cite

Organic ammonium and phosphonium salts exert excellent antimicrobial effects by interacting lethally with bacterial membranes. Particularly, quaternary ammonium lipids have demonstrated efficiency both as gene vectors and antibacterial agents. Here, aiming at finding new antibacterial devices belonging to both classes, we prepared a water-soluble quaternary ammonium lipid (6) and a phosphonium salt (1) by designing a synthetic path where 1 would be an intermediate to achieve 6. All synthesized compounds were characterized by Fourier-transform infrared spectroscopy and Nuclear Magnetic Resonance. Additionally, potentiometric titrations of NH3+ groups 1 and 6 were performed to further confirm their structure by determining their experimental molecular weight. The antibacterial activities of 1 and 6 were assessed first against a selection of multi-drug-resistant clinical isolates of both Gram-positive and Gram-negative species, observing remarkable antibacterial activity of both compounds against Gram-positive isolates of Enterococcus and Staphylococcus genus. Further investigations on a wider variety of strains of these species confirmed the remarkable antibacterial effects of 1 and 6 (MICs = 4–16 and 4–64 µg/mL, respectively), while 24 h-time-killing experiments carried out with 1 on different S. aureus isolates evidenced a bacteriostatic behavior. Moreover, both compounds 1 and 6, at the lower MIC concentration, did not show significant cytotoxic effects when exposed to HepG2 human hepatic cell lines, paving the way for their potential clinical application.

show abstract

Alternatives for the treatment of infections caused by ESKAPE pathogens

Cited by 19 publications

References 30 publications

Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

Antimicrobial Resistance of Eskape Pathogens in Major Burns Patients – One-Year Retrospective Study

Anti Gram-Positive Bacteria Activity of Synthetic Quaternary Ammonium Lipid and Its Precursor Phosphonium Salt

Contact Info

Product

Resources

About