Alternatives for sample pre-treatment and HPLC determination of Ochratoxin A in red wine using fluorescence detection

Tessini, Catherine; Mardones, Claudia; Baer, Dietrich von; Sierra, Matilde; Herlitz, Erika; Saelzer, R.; Silva, Jorge; Torres, Olga

doi:10.1016/j.aca.2009.11.011

Cited by 71 publications

(28 citation statements)

References 22 publications

(36 reference statements)

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“…The results show the response of the fluorescent aptamer is specific for OTA. To further assess whether this method can be applied to the detection of OTA in the complex matrix we made measurement of [39]. Our assay needs to improve the sensitivity for real sample analysis.…”

Section: Specificity Of Fluorescent Sensing Otamentioning

confidence: 99%

Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

2013

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We explored a fluorescent strategy for sensing ochratoxin A (OTA) by using a single fluorophore-labeled aptamer for detection of OTA. This method relied on the change of the fluorescence intensity of the labeled dye induced by the specific binding of the fluorescent aptamer to OTA. Different fluorescein labeling sites of aptamers were screened, including the internal thymine bases, 3′-end, and 5′-end of the aptamer, and the effect of the labeling on the aptamer affinity was investigated. Some fluorophorelabeled aptamers showed a signal-on or signal-off response. With the fluorescent aptamer switch, simple, rapid, and selective sensing of OTA at nanomolar concentrations was achieved. OTA spiked in diluted red wine could be detected, showing the feasibility of the fluorescent aptamer for a complex matrix. This method shows potential for designing aptamer sensors for other targets.

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Section: Specificity Of Fluorescent Sensing Otamentioning

confidence: 99%

Fluorescent sensing ochratoxin A with single fluorophore-labeled aptamer

2013

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“…Toxicology studies show that OTA has nephrotoxicity, hepatic and immune toxicity, and can cause teratogenic effects to a wide variety of mammalian species (Duarte et al, 2004;Lühe et al, 2003). Currently, the most widely applied methods for OTA detection rely on chromatographic approaches (Tessini et al, 2010) and immunoassays (Meulenberg, 2012). Particularly, thin-layer chromatography (TLC) (Pittet and Royer, 2002), high performance liquid chromatography (HPLC) (Ghali et al, 2008) and enzyme-linked immunosorbent assay (ELISA) (Liu et al, 2008) are recommended by the Association of Official Agricultural Chemists and European Commission.…”

Section: Introductionmentioning

confidence: 99%

Electrochemiluminescence biosensor for ultrasensitive determination of ochratoxin A in corn samples based on aptamer and hyperbranched rolling circle amplification

Yang

Zhang

et al. 2015

Biosensors and Bioelectronics

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“…126,127 From its development, IAC 128 has been applied and it is now widely used as a fundamental cleanup step prior to LC-FLD analysis or ELISA, showing higher recoveries and lower detection limits in comparison to SPE columns, 129 although a recent comparison showed that both methods are suitable for OTA analysis in wines. 130 Also, Mycosep and Multisep columns were successfully employed in this field. 131 A combined anion-exchange/reversedphase cleanup was proposed as a cost-effective alternative to IAC, but superior to simple reversed-phase cleanup as it allows elimination of neutral, acidic, and basic impurities.…”

Section: Winementioning

confidence: 99%