Alternatives for Animal Wound Model Systems

Stephens, Philip J.; Caley, Matthew; Peake, Matthew Arnold

doi:10.1007/978-1-62703-505-7_10

Cited by 15 publications

(14 citation statements)

References 145 publications

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“…It was argued that the available diabetic animal wound healing models only demonstrate a short-term impairment in the wound repair process and, therefore, may not reflect the true nature of chronic wounds in humans that can persist for years. Hence these diabetic wound models are actually models for impaired acute wound healing rather than true chronic wounds [36] . Given the life span of current experimental animals (i.e.…”

Section: Discussionmentioning

confidence: 99%

Identification of the Critical Therapeutic Entity in Secreted Hsp90α That Promotes Wound Healing in Newly Re-Standardized Healthy and Diabetic Pig Models

et al. 2014

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Chronic and non-healing skin wounds represent a significant clinical, economic and social problem worldwide. Currently, there are few effective treatments. Lack of well-defined animal models to investigate wound healing mechanisms and furthermore to identify new and more effective therapeutic agents still remains a major challenge. Pig skin wound healing is close to humans. However, standardized pig wound healing models with demonstrated validity for testing new wound healing candidates are unavailable. Here we report a systematic evaluation and establishment of both acute and diabetic wound healing models in pigs, including wound-creating pattern for drug treatment versus control, measurements of diabetic parameters and the time for detecting delayed wound healing. We find that treatment and control wounds should be on the opposite and corresponding sides of a pig. We demonstrate a strong correlation between duration of diabetic conditions and the length of delay in wound closure. Using these new models, we narrow down the minimum therapeutic entity of secreted Hsp90α to a 27-amino acid peptide, called fragment-8 (F-8). In addition, results of histochemistry and immunohistochemistry analyses reveal more organized epidermis and dermis in Hsp90α-healed wounds than the control. Finally, Hsp90α uses a similar signaling mechanism to promote migration of isolated pig and human keratinocytes and dermal fibroblasts. This is the first report that shows standardized pig models for acute and diabetic wound healing studies and proves its usefulness with both an approved drug and a new therapeutic agent.

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Section: Discussionmentioning

confidence: 99%

Identification of the Critical Therapeutic Entity in Secreted Hsp90α That Promotes Wound Healing in Newly Re-Standardized Healthy and Diabetic Pig Models

et al. 2014

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“…Simpler models of wound healing allow studying of specific parts of the wound-healing process [82]. Wound repair within the dermis can, for example be studied by introducing fibroblasts into a solution of collagen [83,84].…”

Section: Three-dimensional In Vitro Modelsmentioning

confidence: 99%

Guided Regeneration of the Human Skin : in vitro and in vivo studies

Nyman¹

2015

Linköping University Medical Dissertations

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“…However, along with cost, availability and ethical restraints, live animal testing is also limited by reproducibility, the ability to offer selective and precise control of independent factors, quantitative interpretation, and interspecies differences. On the other hand, there has been a great impetus to develop alternatives to animal research and testing, including for wound studies (Stephens et al, 2013;Caley et al, 2018). For chronic wound infections, these could potentially include bioengineered in vitro platforms that aim to recapitulate the key components and interactions of the infection microenvironment in a human-relevant and biomimetic manner.…”

Section: Chronic Wound Infectionsmentioning

confidence: 99%