Alternative Splicing of Testis-Specific Lactate Dehydrogenase C Gene in Mammals and Pigeon

Abstract

The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice variants of ldhc were observed in the seven species as a result of deletion of one or more exons or insertion of partial sequence of an intron in the mature mRNA. The deleted exons occur mostly in exons 5, 4, 6, and 3. The insertion of a partial sequence of introns, which resulted in an abnormal stop codon in the inserted intron sequence, was observed only in dog and rat. The deletion of exons also resulted in a reading frame shift and formation of a stop codon in some variants. No alternative splicing was observed for ldha and ldhb genes in testis of yak. Native polyacrylamide gel electrophoresis and Western blot analysis revealed no obvious LDH-C4 activity derived from expressed ldhc variants. Our results demonstrated the widespread and unique existence of alternative splicing of ldhc genes in mammals.

Specific expression of alternatively spliced genes in the turkey (Meleagris gallopavo) reproductive tract revealed their function in spermatogenesis and post-testicular sperm maturation

“…The available data describing splicing events within the reproductive system of the birds focused on 2 genes: chicken DMRT1 and pigeon LDHC ( Zhao et al, 2007 ; Huang et al, 2012 ). The presence of 3 splicing events situated within the DMRT1 locus was also detected in our study; however, no significant expression changes between particular parts of the reproductive tract were recorded.…”

“…A few studies on AS have been reported in relation to bird reproduction ( Zhao et al, 2007 ; Huang et al, 2012 ; Lang et al, 2020 ; Rogers et al, 2021 , Sun et al, 2021 ). AS of testis-specific lactate dehydrogenase C ( LDHC ) and doublesex- and mab-3-related transcription factor 1 ( DMRT1 ) were reported for chicken and pigeon ( Zhao et al, 2007 ; Huang et al, 2012 ).…”

“…A few studies on AS have been reported in relation to bird reproduction ( Zhao et al, 2007 ; Huang et al, 2012 ; Lang et al, 2020 ; Rogers et al, 2021 , Sun et al, 2021 ). AS of testis-specific lactate dehydrogenase C ( LDHC ) and doublesex- and mab-3-related transcription factor 1 ( DMRT1 ) were reported for chicken and pigeon ( Zhao et al, 2007 ; Huang et al, 2012 ). NGS has been recently applied for the characterization of AS events during chicken male germline stem cell differentiation ( Sun et al, 2021 ), sex-specific aspects of the stress response in rock doves ( Lang et al, 2020 ) and the demonstration of the role of AS in the evolution of phenotypic complexity in birds ( Rogers et al, 2021 ).…”

Sodium vitamin C transporter 2 orchestrates lactate metabolism in mouse Sertoli cells

“…4F). The lactate dehydrogenase (LDH), mainly consisting of LDHA and/or LDHB subunits, catalyzes the final step of lactate synthesis, namely conversion of pyruvate to lactate (Huang et al 2012). Concerning the testicular function of LDHA, two aspects should be emphasized.…”