Alternative splicing of human cyclin E

Sewing, A.; Rönicke, Volker; Bürger, Christiane; Funk, Martin; Müller, Rolf

doi:10.1242/jcs.107.2.581

Cited by 63 publications

(2 citation statements)

References 46 publications

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“…The accumulation of plasma growth-related hormones may contribute to the upregulation of cyclin E protein in salinity administrations [46,47], especially in the 15 ppt and 25 ppt salinity treatment groups. Cyclin E protein in Hong Kong oysters is expressed as two isoforms, which may be derived by alternate RNA splicing [48] or post-translational modifications, such as phosphorylation [49]. Cyclin E is strongly concentrated in the nucleus of Xenopus [27], which is consistent with the cyclin sub-cellular location in Hong Kong oysters.…”

Section: Discussionsupporting

confidence: 58%

The Identification of a Cell Cycle Regulation Gene Cyclin E from Hong Kong Oysters (Crassostrea hongkongensis) and Its Protein Expression in Response to Salinity Stress

Qiu,

Wang,

Yan

et al. 2024

Fishes

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Hong Kong oysters (Crassostrea hongkongensis) are an important marine bivalve with nutritional and commercial value. The expanded off-bottom farming scale in recent years makes the oysters more susceptible to exposure to abiotic stresses, such as salinity stress, an important environmental factor that has been proven to have significant effects on oyster growth and development. However, the molecular mechanism is still unclear. Cyclin E is an important protein in the process of cell cycle regulation that is indispensable for propelling G1/S phase transition in a dose-dependent manner. In order to investigate whether the salinity stress affects cyclin E expression in oysters, the cDNA sequence of C. hongkongensis cyclin E (Ch-CCNE) was isolated from a gill cDNA library, and the 2.8 kbp length cDNA fragment contained a complete open reading frame (ORF) encoding 440 amino acid residues. Ch-CCNE mRNA was highly expressed in the gonad and low in the adductor mussel, mantle, gill, labial palp, and digestive gland. The recombinant CCNE protein was expressed and purified in a pET32a(+)-CCNE/Escherichia coli BL21(DE3) system via IPTG induction and was used for generating mice anti-Ch-CCNE antiserums. Western blot analysis showed that the CCNE protein in the gill was maintained at low expression levels under either hypo- (5 ppt) or hyper- (35 ppt) salinity, and could be produced at high levels under appropriate salinity during a 10-day exposure period. The immuno-localization indicated that the Ch-CCNE protein was distributed in the nucleus. These results suggested that either hypo- or hyper-salinity stress could inhibit the CCNE expression of Hong Kong oysters and their negative impact on cell division and proliferation.

show abstract

Section: Discussionsupporting

confidence: 58%

The Identification of a Cell Cycle Regulation Gene Cyclin E from Hong Kong Oysters (Crassostrea hongkongensis) and Its Protein Expression in Response to Salinity Stress

Qiu,

Wang,

Yan

et al. 2024

Fishes

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“…Nevertheless, an inhibitor specific splicing factor for CCND1 splice correction needs to be identified. Besides CCND1, the cyclin family members including CCNA [202], CCNB [203], and CCNE [204] also have multiple splice isoforms, but it is not clear that the multiple splice isoforms of the other family members play a finely coordinated biological role. Thus, there still exists much work in future research on this gene.…”

Section: Discussionmentioning

confidence: 99%

Aberrant Cyclin D1 splicing in cancer: from molecular mechanism to therapeutic modulation

Wang

Zhang

et al. 2023

Cell Death Dis

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Cyclin D1 (CCND1), a crucial mediator of cell cycle progression, possesses many mutation types with different mutation frequencies in human cancers. The G870A mutation is the most common mutation in CCND1, which produces two isoforms: full-length CCND1a and divergent C-terminal CCND1b. The dysregulation of the CCND1 isoforms is associated with multiple human cancers. Exploring the molecular mechanism of CCND1 isoforms has offer new insight for cancer treatment. On this basis, the alterations of CCND1 gene are described, including amplification, overexpression, and mutation, especially the G870A mutation. Subsequently, we review the characteristics of CCND1 isoforms caused by G870A mutation. Additionally, we summarize cis-regulatory elements, trans-acting factors, and the splice mutation involved in splicing regulation of CCND1. Furthermore, we highlight the function of CCND1 isoforms in cell cycle, invasion, and metastasis in cancers. Importantly, the clinical role of CCND1 isoforms is also discussed, particularly concerning prognosis, chemotherapy, and radiotherapy. Last, emphasis is given to the corrective strategies that modulate the cancerous CCND1 isoforms. Thus, it is highlighting significance of aberrant isoforms of CCND1 as targets for cancer therapy.

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Tumorigenesis and neurodegeneration: two sides of the same coin?

Staropoli

2008

BioEssays

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SummaryDysregulation of genes that control cell-cycle progression and DNA repair is a hallmark of tumorigenesis. It is becoming increasingly apparent, however, that these defects also contribute to degeneration of post-mitotic neurons under certain conditions. The gene for ataxiatelangiectasia mutated (ATM) is a prototype for this dual mechanism of action, with loss-of-function mutations causing not only selective degeneration of cerebellar neurons but also increased susceptibility to breast cancer and hematologic malignancy. Increased dosage of amyloid precursor protein in Down syndrome (trisomy 21) predisposes to dementia of Alzheimer type and may also contribute to acute leukemia and transient myeloproliferative disorder. The gene parkin, loss-of-function mutations in which account for about half of cases of early-onset Parkinson disease, has been identified as a candidate tumor suppressor gene by several groups. Parkin is deleted or downregulated in several tumor types, and its re-expression sensitizes derivative cell lines to inhibitors of cell-cycle progression. The overlap of molecular pathways implicated in cancer and neurodegeneration challenges long-held notions about differentiated cellular states and may open the door to novel therapeutic approaches to both groups of disorders.

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Alternative splicing of human cyclin E

Cited by 63 publications

References 46 publications

The Identification of a Cell Cycle Regulation Gene Cyclin E from Hong Kong Oysters (Crassostrea hongkongensis) and Its Protein Expression in Response to Salinity Stress

The Identification of a Cell Cycle Regulation Gene Cyclin E from Hong Kong Oysters (Crassostrea hongkongensis) and Its Protein Expression in Response to Salinity Stress

Aberrant Cyclin D1 splicing in cancer: from molecular mechanism to therapeutic modulation

Tumorigenesis and neurodegeneration: two sides of the same coin?

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