Alternative splicing of a single precursor mRNA generates two subtypes of Gonadotropin-Releasing Hormone receptor orthologues and their variants in the bivalve mollusc Crassostrea gigas

Rodet, Franck; Lelong, Christophe; Dubos, Marie-Pierre; Favrel, Pascal

doi:10.1016/j.gene.2008.01.022

Cited by 27 publications

(14 citation statements)

References 43 publications

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“…Both truncated isoforms resulted from alternative splicing of the full-length ampkα gene. Alternative splicing was already reported for some genes in C. gigas and the spliced variants may present a tissue-specific expression and a specific function (Rodet et al 2008).…”

Section: Crassostrea Gigas Ampkα Isoformsmentioning

confidence: 89%

Regulation of a truncated isoform of AMP-activated protein kinase α (AMPKα) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas

et al. 2013

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AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism was maintained over the 6 h of hypoxia, as mitochondrial citrate synthase activity remained constant. In contrast, in striated muscle, hypoxia did not induce any significant modification of neither truncated AMPKα nor AKT protein content, and citrate synthase activity was altered after 6 h of hypoxia. Together, our results demonstrate that hypoxia response is specific to muscle type in Pacific oyster, and that truncated AMPKα and AKT proteins might be involved in maintaining aerobic metabolism in smooth muscle. Such regulation might occur in vivo during tidal intervals that cause up to 6 h of hypoxia.

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Section: Crassostrea Gigas Ampkα Isoformsmentioning

confidence: 89%

Regulation of a truncated isoform of AMP-activated protein kinase α (AMPKα) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas

et al. 2013

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“…The gonadotropin releasing hormone receptor of the Pacific oyster Crassostrea gigas is involved in the control of gametogenesis (Rodet et al 2008), as gonadotropin releasing hormone receptors are in vertebrates. It has fairly strong identity with the insect AKH receptors up to 44% (Figure 3), while with the receptor from another mollusk, Octopus vulgaris, it is only 27% identical, and with all the other non-insects 34% or less.…”

Section: Discussionmentioning

confidence: 99%

The Putative AKH Receptor of the Tobacco Hornworm,Manduca sexta, and Its Expression

Ziegler

Isoe

Moore

et al. 2011

Journal of Insect Science

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Adipokinetic hormones are peptide hormones that mobilize lipids and/or carbohydrates for flight in adult insects and activate glycogen Phosphorylase in larvae during starvation and during molt. We previously examined the functional roles of adipokinetic hormone in Manduca sexta L. (Lepidoptera: Sphingidae). Here we report the cloning of the full-length cDNA encoding the putative adipokinetic hormone receptor from the fat body of M. sexta. The sequence analysis shows that the deduced amino acid sequence shares common motifs of G protein-coupled receptors, by having seven hydrophobic transmembrane segments. We examined the mRNA expression pattern of the adipokinetic hormone receptor by quantitative Real-Time PCR in fat body during development and in different tissues and found the strongest expression in fat body of larvae two days after molt to the fifth instar. We discuss these results in relation to some of our earlier results. We also compare the M. sexta adipokinetic hormone receptor with the known adipokinetic hormone receptors of other insects and with gonadotropin releasing hormone-like receptors of invertebrates.

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“…They also demonstrated a decrease in progeny of C. elegans after knockdown of GnRHR, using RNA interference on the Ce-GnRHR or the AKH-GnRHR precursor. Rodet et al (2008) reported the first splice variants for an invertebrate GnRH-receptor. They cloned and characterized four alternately spliced mRNAs for GnRH-receptor orthologs from the C. gigas that may differ in ligand specificity and functions.…”

Section: Akhr Genes In a Aegyptimentioning

confidence: 99%

The adipokinetic hormone system in Culicinae (Diptera: Culicidae): Molecular identification and characterization of two adipokinetic hormone (AKH) precursors from Aedes aegypti and Culex pipiens and two putative AKH receptor variants from A. aegypti

Kaufmann

Merzendorfer

GÄde

2009

Insect Biochemistry and Molecular Biology

114

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Alternative splicing of a single precursor mRNA generates two subtypes of Gonadotropin-Releasing Hormone receptor orthologues and their variants in the bivalve mollusc Crassostrea gigas

Cited by 27 publications

References 43 publications

Regulation of a truncated isoform of AMP-activated protein kinase α (AMPKα) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas

Regulation of a truncated isoform of AMP-activated protein kinase α (AMPKα) in response to hypoxia in the muscle of Pacific oyster Crassostrea gigas

The Putative AKH Receptor of the Tobacco Hornworm,Manduca sexta, and Its Expression

The adipokinetic hormone system in Culicinae (Diptera: Culicidae): Molecular identification and characterization of two adipokinetic hormone (AKH) precursors from Aedes aegypti and Culex pipiens and two putative AKH receptor variants from A. aegypti

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