Alternative splicing and tissue expression of CIB4 gene in sheep testis

Yu, Yan; Yuan, Zhongwei; Song, Xuemei; Jin, Mei; Guan, Qun; Zhang, Qiyao; Li, Shangang; Wei, Caihong; Lu, Guobin; Zhang, Ju; Ren, Hangxing; Sheng, Xihui; Wang, Chuduan; Du, Lixin

doi:10.1016/j.anireprosci.2010.01.004

Cited by 12 publications

(18 citation statements)

References 34 publications

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“…Failure in this stage can result in a non-viable pregnancy, a fact that would explain this association. Genes associated with male fertility [32, 36, 37, 56] were also found within the top 20 windows ( CIB4 , UCN , and PGAM1 ), a finding that would explain the genetic correlation between male and female reproductive traits as reported by Irano et al [11]. Another possible explanation is that sperm quality influences pregnancy success.…”

Section: Resultsmentioning

confidence: 67%

“…The subsequent window of BTA11 (72-73Mb) harbors important genes previously reported to be associated with reproductive events, such as SLC30A3 [30] and CAD [31] described as estrogen receptors, CIB4 associated with male sheep fecundity [32], EMILIN1 associated with placentation and trophoblast invasion in the uterine wall [33], TCF23 which plays an important role in endometrial decidualization [34], UCN associated with steroidogenesis and maintenance of placental function which is expressed in mature mouse spermatozoa and rat epididymis [35, 36, 37], and IFT172 , PREB and SNX17 which are associated with neural development [38, 39, 40, 41]. Furthermore, window 49-50Mb of BTA17 has been reported to be associated with age at first calving in Nellore cows [13].…”

Section: Resultsmentioning

confidence: 99%

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Genome-wide association study provides strong evidence of genes affecting the reproductive performance of Nellore beef cows

et al. 2017

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Reproductive traits are economically important for beef cattle production; however, these traits are still a bottleneck in indicine cattle since these animals typically reach puberty at older ages when compared to taurine breeds. In addition, reproductive traits are complex phenotypes, i.e., they are controlled by both the environment and many small-effect genes involved in different pathways. In this study, we conducted genome-wide association study (GWAS) and functional analyses to identify important genes and pathways associated with heifer rebreeding (HR) and with the number of calvings at 53 months of age (NC53) in Nellore cows. A total of 142,878 and 244,311 phenotypes for HR and NC53, respectively, and 2,925 animals genotyped with the Illumina Bovine HD panel (Illumina®, San Diego, CA, USA) were used in GWAS applying the weighted single-step GBLUP (WssGBLUP) method. Several genes associated with reproductive events were detected in the 20 most important 1Mb windows for both traits. Significant pathways for HR and NC53 were associated with lipid metabolism and immune processes, respectively. MHC class II genes, detected on chromosome 23 (window 25-26Mb) for NC53, were significantly associated with pregnancy success of Nellore cows. These genes have been proved previously to be associated with reproductive traits such as mate choice in other breeds and species. Our results suggest that genes associated with the reproductive traits HR and NC53 may be involved in embryo development in mammalian species. Furthermore, some genes associated with mate choice may affect pregnancy success in Nellore cattle.

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Section: Resultsmentioning

confidence: 67%

Section: Resultsmentioning

confidence: 99%

Genome-wide association study provides strong evidence of genes affecting the reproductive performance of Nellore beef cows

et al. 2017

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“…QRT-PCR was performed using SYBR Green (Fast SYBR® Green Master Mix; Applied Biosystems) to detect mRNA relative expression, with primers specifically targeting sheep fas ligand (FASL) , tumor protein p53 ( TP53) and caspase3 ( CASP3 ) genes designed using NCBI primer blast ( http://www.ncbi.nlm.nih.gov/tools/primerblast/index.cgi?LINK_LOC=BlastHome ). Primer sequences for GAPDH were obtained from a published source ( Table S1 ) 30 . Fluorescence signal was detected with StepOnePlus™ Real-Time PCR System (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis

Guan

Liang

Hawken

et al. 2015

Sci Rep

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We tested whether reductions in spermatozoal quality induced by under-nutrition are associated with increased germ cell apoptosis and disrupted spermatogenesis, and whether these effects are mediated by small RNAs. Groups of 8 male sheep were fed for a 10% increase or 10% decrease in body mass over 65 days. Underfeeding increased the number of apoptotic germ cells (P < 0.05) and increased the expression of apoptosis-related genes (P < 0.05) in testicular tissue. We identified 44 miRNAs and 35 putative piRNAs that were differentially expressed in well-fed and underfed males (FDR < 0.05). Some were related to reproductive system development, apoptosis (miRNAs), and sperm production and quality (piRNAs). Novel-miR-144 (miR-98), was found to target three apoptotic genes (TP53, CASP3, FASL). The proportion of miRNAs as a total of small RNAs was greater in well-fed males than in underfed males (P < 0.05) and was correlated (r = 0.8, P < 0.05) with the proportion of piRNAs in well-fed and underfed males. In conclusion, the reductions in spermatozoal quality induced by under-nutrition are caused, at least partly, by disruptions to Sertoli cell function and increased germ cell apoptosis, mediated by changes in the expression of miRNAs and piRNAs.

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“…$$ P<0.01; $ P<0.05; # P<0.05. Differences between three groups were determined by one-way ANOVA (n=5 patients in each group) fertility [14], while another family member, CIB4, was only detected in sheep testes, and the short spliced form of this transcript, S-CIB4, could only be detected in a breed of sheep with high fecundity [15]. Furthermore, the present study identified that there was an altered CIB1 profile in the sperm of infertile patents and fertile controls.…”

Section: Discussionmentioning

confidence: 53%

“…CDK1 was found to be overexpressed in the testes of Cib1 −/− male mice and cells isolated from testis tubules, and it is confirmed that its overexpression was unique to the testis not a general phenomenon, it was considered that CIB1 may interact with CDK1, albeit transiently [13]. In our previous study, CIB1 was widely expressed in different tissues in sheep, with the highest level being observed in the testis [14]; moreover, another member of this family, CIB4, may be linked to the high fecundity of a sheep breed indigenous to China [15]. These studies indicated that CIB1 may play an important role in human male fertility.…”

Section: Introductionmentioning

confidence: 79%

Calcium- and integrin-binding protein-1 is down-regulated in the sperm of patients with oligoasthenozoospermia

Sun

Guan

Wen

et al. 2014

J Assist Reprod Genet

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Purpose The aim of this study was to determine whether altered expression and distribution of calcium-and integrinbinding protein-1 (CIB1) is involved in the pathogenesis of patients with oligoasthenozoospermia. Methods Sperm samples were obtained from 25 infertile Chinese men who had failed to achieve conception after a period of 1-2 y and had been referred to the Reproductive Laboratory of the second hospital affiliated to the Shandong University of Traditional Chinese Medicine. Participants were divided into two groups: oligoasthenozoospermia (n=13) and asthenozoospermia (n=12); as a third group, fertile men (n=19) were included as controls. The expression levels of mRNA and protein levels of CIB1 and cyclin-dependent kinase 1 (CDK1) were measured using qRT-PCR and western blotting. Results mRNA and protein expression levels of CIB1 were decreased in the oligoasthenozoospermia patients. Interestingly mRNA and protein expression levels of CDK1 were increased in the oligoasthenozoospermia patients. Conclusion The results of the present study indicate that that CIB1 may be involved in the pathogenesis of oligoasthenozoospermia by the CDK1 signaling pathway.

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Alternative splicing and tissue expression of CIB4 gene in sheep testis

Cited by 12 publications

References 34 publications

Genome-wide association study provides strong evidence of genes affecting the reproductive performance of Nellore beef cows

Genome-wide association study provides strong evidence of genes affecting the reproductive performance of Nellore beef cows

Roles of small RNAs in the effects of nutrition on apoptosis and spermatogenesis in the adult testis

Calcium- and integrin-binding protein-1 is down-regulated in the sperm of patients with oligoasthenozoospermia

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