Alternative scenarios of starvation-induced adaptation in Pectobacterium atrosepticum

Petrova, Olga; Gorshkov, Vladimir; Sergeeva, Iuliia; Daminova, Amina; Ageeva, Marina; Gogolev, Yu. V.

doi:10.1016/j.resmic.2016.01.009

Cited by 7 publications

(7 citation statements)

References 34 publications

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“…This is important in the aspect of microbial adaptation and the ability of phytopathogens including Pectobacterium to adapt and survive under unfavorable conditions in various ecological environments like sea water, fresh surface water, ground water, soil, etc. These adaptations result in the formation of resistance to various stress factors: oxidative stress, heat shock, and antibiotic treatment ( Petrova et al, 2016 ). Genes for resistance to antibiotics and toxic compounds such as fluoroquinolones and beta lactamase have been identified in Pcc ICMP 5702.…”

Section: Discussionmentioning

confidence: 99%

Genome Wide Analysis of the Potato Soft Rot Pathogen Pectobacterium carotovorum Strain ICMP 5702 to Predict Novel Insights into Its Genetic Features

et al. 2022

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Pectobacterium carotovorum subsp. carotovorum (Pcc) is a gram-negative, broad host range bacterial pathogen which causes soft rot disease in potatoes as well as other vegetables worldwide. While Pectobacterium infection relies on the production of major cell wall degrading enzymes, other virulence factors and the mechanism of genetic adaptation of this pathogen is not yet clear. In the present study, we have performed an in-depth genome-wide characterization of Pcc strain ICMP5702 isolated from potato and compared it with other pathogenic bacteria from the Pectobacterium genus to identify key virulent determinants. The draft genome of Pcc ICMP5702 contains 4,774,457 bp with a G + C content of 51.90% and 4,520 open reading frames. Genome annotation revealed prominent genes encoding key virulence factors such as plant cell wall degrading enzymes, flagella-based motility, phage proteins, cell membrane structures, and secretion systems. Whereas, a majority of determinants were conserved among the Pectobacterium strains, few notable genes encoding AvrE-family type III secretion system effectors, pectate lyase and metalloprotease in addition to the CRISPR-Cas based adaptive immune system were uniquely represented. Overall, the information generated through this study will contribute to decipher the mechanism of infection and adaptive immunity in Pcc.

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Section: Discussionmentioning

confidence: 99%

Genome Wide Analysis of the Potato Soft Rot Pathogen Pectobacterium carotovorum Strain ICMP 5702 to Predict Novel Insights into Its Genetic Features

et al. 2022

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“…Additionally, since bacteria are able to resist stressors applying alternative adaptive reactions, many of which lead to the formation of cross-protection [25, 26], it would be interesting to compare transcriptome profiles of cross-protected phenotypes formed in the course of realization of different adaptive strategies. Valuable information may be also obtained by analyzing stress-induced transcriptomes of mutant bacteria that are unable to form cross-protection phenotypes under stress conditions (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…Representatives of harmful soft-rot Enterobacteriaceace ( Pectobacterium and Dickeya species) that cause serious plant damages all over the world are known to be able to survive, besides plants, in soil, clouds, sea water, fresh surface water, ground water, insects, and mollusks [22]. Moreover, pectobacteria may apply alternative types of adaptive reactions depending on various exogenous and endogenous factors [23–26]. Some of these reactions were shown to result in the formation of resistance to multiple stress factors: oxidative stress, heat shock, and antibiotic treatment [26]; however, the time necessary for the formation of the cross-protected phenotype was not determined.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, pectobacteria may apply alternative types of adaptive reactions depending on various exogenous and endogenous factors [23–26]. Some of these reactions were shown to result in the formation of resistance to multiple stress factors: oxidative stress, heat shock, and antibiotic treatment [26]; however, the time necessary for the formation of the cross-protected phenotype was not determined. Altogether, pectobacteria represent suitable model microorganisms for studying microbial adaptation.…”

Section: Introductionmentioning

confidence: 99%

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Global Gene Expression Analysis of Cross-Protected Phenotype of Pectobacterium atrosepticum

et al. 2017

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The ability to adapt to adverse conditions permits many bacterial species to be virtually ubiquitous and survive in a variety of ecological niches. This ability is of particular importance for many plant pathogenic bacteria that should be able to exist, except for their host plants, in different environments e.g. soil, water, insect-vectors etc. Under some of these conditions, bacteria encounter absence of nutrients and persist, acquiring new properties related to resistance to a variety of stress factors (cross-protection). Although many studies describe the phenomenon of cross-protection and several regulatory components that induce the formation of resistant cells were elucidated, the global comparison of the physiology of cross-protected phenotype and growing cells has not been performed. In our study, we took advantage of RNA-Seq technology to gain better insights into the physiology of cross-protected cells on the example of a harmful phytopathogen, Pectobacterium atrosepticum (Pba) that causes crop losses all over the world. The success of this bacterium in plant colonization is related to both its virulence potential and ability to persist effectively under various stress conditions (including nutrient deprivation) retaining the ability to infect plants afterwards. In our previous studies, we showed Pba to be advanced in applying different adaptive strategies that led to manifestation of cell resistance to multiple stress factors. In the present study, we determined the period necessary for the formation of cross-protected Pba phenotype under starvation conditions, and compare the transcriptome profiles of non-adapted growing cells and of adapted cells after the cross-protective effect has reached the maximal level. The obtained data were verified using qRT-PCR. Genes that were expressed differentially (DEGs) in two cell types were classified into functional groups and categories using different approaches. As a result, we portrayed physiological features that distinguish cross-protected phenotype from the growing cells.

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“…Bacteria from Pectobacterium genus being harmful soil‐borne plant pathogens experience both aggressive soil environment during non‐vegetative period and plant defense responses when inside the host. These bacteria are almost ubiquitous and apply various adaptive programs to persist out of their hosts . Getting into the host induces massive production of virulence determinants, including the major ones − pectate lyases, cellulases and proteases leading to extensive tissue maceration and formation of “soft rots” .…”

Section: Introductionmentioning

confidence: 99%

Polyphenol oxidase fromPectobacterium atrosepticum: identification and cloning of gene and characteristics of the enzyme

et al. 2017

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In the present study, we attempted to elucidate if the harmful phytopathogenic bacteria of Pectobacterium genus (P. atrosepticum) possess the enzymes for oxidation of phenolic compounds. Polyphenol oxidase (laccase) activity was revealed in P. atrosepticum cell lysates. Using bioinformatic analysis, an ORF encoding a putative copper-containing polyphenol oxidase of 241 amino acids with a predicted molecular mass of 25.9 kDa was found. This protein (named Pal1) shares significant level of identity with laccases of a new type described for several bacterial species. Cloning and expression of the pal1 gene and the analysis of corresponding recombinant protein confirmed that Pal1 possessed laccase activity. The recombinant Pal1 protein was characterized in terms of substrate specificity, kinetic parameters, pH and temperature optimum, sensitivity to inhibitors and metal content. Pal1 demonstrated alkali- and thermo-tolerance. The kinetic parameters K and kcat for 2,6-dimethoxyphenol were 0.353 ± 0.062 mM and 98.79 ± 4.9 s , respectively. The protein displayed high tolerance to sodium azide, sodium fluoride, NaCl, SDS and cinnamic acid. The transcript level of the pal1 gene in P. atrosepticum was shown to be induced by plant-derived phenolic compound (ferulic acid) and copper sulfate.

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Alternative scenarios of starvation-induced adaptation in Pectobacterium atrosepticum

Cited by 7 publications

References 34 publications

Genome Wide Analysis of the Potato Soft Rot Pathogen Pectobacterium carotovorum Strain ICMP 5702 to Predict Novel Insights into Its Genetic Features

Genome Wide Analysis of the Potato Soft Rot Pathogen Pectobacterium carotovorum Strain ICMP 5702 to Predict Novel Insights into Its Genetic Features

Global Gene Expression Analysis of Cross-Protected Phenotype of Pectobacterium atrosepticum

Polyphenol oxidase fromPectobacterium atrosepticum: identification and cloning of gene and characteristics of the enzyme

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