Alternative Pathway of Complement Activation in Full Term and Premature Infants

Strunk, Robert C.; Fenton, Lawrence J.; Gaines, John A.

doi:10.1203/00006450-197905000-00013

Cited by 48 publications

(33 citation statements)

References 9 publications

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“…In the few reports also including studies of complement in low birth weight infants, classical as well as alternative complement activity have generally been found to be lower in low birth weight than in normal term infants (1,6,8,14,16). But it is not clear from these studies whether the defect of complement activity in low birth weight infants is the result of premature birth (6,8) or of intrauterine growth retardation (1,14,16). The purposes of the present study were to compare complement activity of both pathways in AGA and SGA low birth weight newborns, and to determine the influence of birth weight and gestational age on complement development in such infants.…”

Section: Discussionmentioning

confidence: 99%

“…Complement activity is lower in low birth weight infants than in term infants; however, the relationship of gestational age and/ or birth weight to the level of complement activity is still controversial (1,6,8,14,16). The purpose of the present study was to investigate the effects of birth weight and gestational age on complement development (CH50 and kinetics of both CP and AP, C3, and Factor B levels) in low birth weight infants divided into appropriate and small for gestational age groups.…”

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confidence: 99%

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Activity of Classical and Alternative Pathways of Complement in Preterm and Small for Gestational Age Infants

et al. 1984

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SummaryComplement activity was compared in 50 low birth weight infants divided into appropriate and small for gestational age groups; the influence of birth weight and gestational age on complement development was also investigated. CHW and kinetics (tHso) of both classical and alternative pathway activity of complement, C3, and Factor B levels were significantly higher in small for gestational age infants (classical pathway CHs, 630 HU/ml f 184 SD, C P tHs, 77 min + 47; aternative pathway CHS0, 44.8 HU/ml & 11.3; AP tHso, 56 min f 43; C3, 73.98 mg/dl f 12.68; and Factor B, 13.17 mg/dl 2 3.67) than in weight-matched appropriate for gestational age infants (CP CHW, 523 HU/ml 2 152; C P tH9, 105 min + 49; AP CHW, 38.8 HU/ml f 13; AP tHW, 90 min 2 53; C3, 58.14 mg/dl f 9.43; and Factor B, 9.32 mg/dl+ 1.73). Complement values were lower in low birth weight infants than in adult controls ( P < 0.001 in all cases). All complement parameters were mainly correlated with gestational age; CHso values of the classical and alternative pathways were also highly correlated with each other

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Activity of Classical and Alternative Pathways of Complement in Preterm and Small for Gestational Age Infants

et al. 1984

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“…These defects were unlikely to have been solely a function of the patients age because normal age-matched controls had levels of complement components and activity which did not differ significantly from those in published adult series (9,10,22,28). Whilst there is an increased incidence of both opsonisation deficiency (19) and complement activation (24) in young, and especially preterm infants, none of the children studied were of similar age or gestation to these groups. It is also unlikely that the defects were primary or congenital because they were multiple, reversible and unassociated with previous symptoms suggesting opsonisation or complement deficiency (1, 3, 23).…”

Section: Discussionmentioning

confidence: 75%

Yeast Opsonisation and Complement in Children with Liver Disease. Analysis of 69 Cases

et al. 1983

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“…Strunk et al (18) have demonstrated that the classical and the alternative pathway activation sequences are deficient to the same degree in newborn compared with adult sera. But previous studies in which the degree of depression of complement function in neonatal sera was related td deficient opsonization of a bacterial particle have shown that alternative rather than classical pathway opsonic function was defective.…”

Section: Discussionmentioning

confidence: 99%

Deficient Classical Complement Pathway Activity in Newborn Sera

et al. 1983

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SummaryThe bactericidal activity of 20 maternal-neonatal paired sera was assessed employing a clinical type Ia, group B streptococcal isolate, strain 515, known to be opsonized by the classical complement pathway in a non-antibody dependent fashion. Twelve neonatal sera had efticient bactericidal activity for this isolate (mean 96%, range 91-99%) whereas eight had significantly lower bactericidal indices (mean 29%, range 0-54%) ( p < 0.001). The mean bactericidal index for 20 maternal sera (88%) did not differ from that of 20 adult control sera (91%). Bactericidal activity was not influenced by the concentration of antibody to the capsular polysaccharide antigen of type Ia, group B Streptococcus present in these sera. When the classical pathway of selected neonatal sera with high or low bactericidal activity was inactivated by MgEGTA chelation of C1, bacterial growth was observed uniformly when concentrations of specific antibody were low. The bactericidal index of neonatal sera correlated significantly with the total hemolytic complement titer ( r = 0.611, P < 0.01). The mean levels of each complement component or control protein assessed by radial immunodiffusion (Clq, C4, C3, B, H, and I) were lower in neonatal sera with low than in those with efficient bactericidal activity, and levels of Clq and H were depressed significantly (P < 0.025 and <0.001, respectively). Hemolytic titers of C4 but not C1 or C2 were also significantly lower for low than for selected high-killing neonatal sera (P < 0.05). Bactericidal activity in neonatal serum with a bactericidal index of zero was restored in a dose-dependent fashion by the addition of fresh frozen plasma. Abbreviations BI, bactericidal index CHSO, whole complement activity EGTA, ethyleneglycoltetracetate FFP, fresh frozen plasma GVB, Veronal-buffered saline containing 0.1% gelatin GVB++, GVB containing 0.5 mM Mg++ and 0.15 mM Ca++ MgEGTA, GVB supplemented to 4 mM Mg++ and 16 mM EGTA adjusted to pH 7.5 OD, optical density THB, Todd-Hewitt brothThe levels of individual classical complement pathway components in neonatal sera have been shown to be significantly lower than those in adults (1,6-7, 11-12, 19). Davis et al. (6) have shown that by the age of 6 months the mean concentrations of C2 and C4 have increased to levels not significantly different from those of adults, but Clq and C3 concentrations remain low. Few studies have compared the functional capacity of the classical pathway for a pathogen common in the neonate with the degree of depression of individual classical pathway components. We have shown previously that bactericidal activity in adult sera for a variety of clinical isolates of type Ia, group B streptococci was mediated by the classical complement pathway in a non-antibody dependent fashion (2). Both adult sera deficient in specific antibody and agammaglobulinemic serum, which had no detectable antibody to the capsular polysaccharide antigen of type la, group B Streptococcus supported efficient bactericidal activity for each of 18 clinical type Ia...

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Alternative Pathway of Complement Activation in Full Term and Premature Infants

Cited by 48 publications

References 9 publications

Activity of Classical and Alternative Pathways of Complement in Preterm and Small for Gestational Age Infants

Activity of Classical and Alternative Pathways of Complement in Preterm and Small for Gestational Age Infants

Yeast Opsonisation and Complement in Children with Liver Disease. Analysis of 69 Cases

Deficient Classical Complement Pathway Activity in Newborn Sera

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