Alternative oxidase 1 (Aox1) gene expression in roots of Medicago truncatula is a genotype-specific component of salt stress tolerance

Mhadhbi, Haythem; Fotopoulos, Vasileios; Mylona, Photini V.; Jebara, Moez; Aouani, Mohamed Elarbi; Polidoros, Alexios N.

doi:10.1016/j.jplph.2012.08.017

Cited by 46 publications

(27 citation statements)

References 26 publications

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“…3). The increased capacity of AOP observed in TN1.11 after 3 days of salt treatment may be explained by the increase of AOX1a expression observed in this genotype by Mhadhbi and Fotopoulos, (2013). Nevertheless, neither a correlation between root respiration nor the activity of the AOP with the responsiveness to severe salt stress has Table 1.…”

Section: Discussionmentioning

confidence: 88%

“…In the present study, the effect of sudden severe salinity has been studied in three Medicago truncatula genotypes (TN1.11, A17 and TN6.18) that in previous studies showed different response to salinity based on the induction and sustained expression of antioxidant mechanisms (Mhadhbi et al ., ; Mhadhbi and Fotopoulos, ). Leaf gas exchange and water content parameters were determined as physiological indicators of the salinity stress level, and in vivo leaf and root respiration has been measured using the oxygen isotope fractionation technique, which is the most reliable technique for studying the in vivo activities of COP and AOP (Day et al ., ).…”

Section: Introductionmentioning

confidence: 98%

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Salinity tolerance is related to cyanide‐resistant alternative respiration in Medicago truncatula under sudden severe stress

Del‐Saz

Florez‐Sarasa

Clemente‐Moreno

et al. 2016

Plant Cell & Environment

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Salt respiration is defined as the increase of respiration under early salt stress. However, the response of respiration varies depending on the degree of salt tolerance and salt stress. It has been hypothesized that the activity of the alternative pathway may increase preventing over-reduction of the ubiquinone pool in response to salinity, which in turn can increase respiration. Three genotypes of Medicago truncatula are reputed as differently responsive to salinity: TN1.11, A17 and TN6.18. We used the oxygen-isotope fractionation technique to study the in vivo respiratory activities of the cytochrome oxidase pathway (COP) and the alternative oxidase pathway (AOP) in leaves and roots of these genotypes treated with severe salt stress (300 mM) during 1 and 3 days. In parallel, AOX capacity, gas exchange measurements, relative water content and metabolomics were determined in control and treated plants. Our study shows for first time that salt respiration is induced by the triggered AOP in response to salinity. Moreover, this phenomenon coincides with increased levels of metabolites such as amino and organic acids, and is shown to be related with higher photosynthetic rate and water content in TN6.18.

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Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 98%

Salinity tolerance is related to cyanide‐resistant alternative respiration in Medicago truncatula under sudden severe stress

Del‐Saz

Florez‐Sarasa

Clemente‐Moreno

et al. 2016

Plant Cell & Environment

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“…Thus, the induction pattern of these genes in maize may serve as a selective marker for diverse mitochondrial defects. Mhadhbia et al (2013) analyzed AOX1 gene expression levels in genotypes of Medicago truncatula with different levels of tolerance to salt stress. The results of this study revealed specific induction of AOX1 in the roots of tolerant genotypes; genotypes showing higher AOX1 expression had fewer biochemical alterations, such as lipid peroxidation and production of ROS.…”

Section: Resultsmentioning

confidence: 99%

Expression of genes related to tolerance to low temperature for maize seed germination

Silva-Neta¹,

Pinho²,

Veiga³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The aim of this study was to characterize maize lines tolerant to cold temperatures during the germination process. Seeds from lines with different levels of tolerance to low temperatures were used; 3 lines were classified as tolerant and 3 as susceptible to low germination temperatures. A field was set up to multiply seeds from selected lines. After the seeds were harvested and classified, we conducted physiological tests and analyzed fatty acid content of palmitic, stearic, oleic, linoleic, linolenic, and eicosenoic acids. In proteomic analysis, the expression of heat-resistant proteins, including catalase, peroxidase, esterase, superoxide dismutase, and α-amylase, were evaluated. Transcript analysis was used to measure the expression of the genes AOX1, AOX2, ZmMPK-17, and ZmAN-13. The material showing the highest susceptibility to low germination temperatures contained high saturated fatty acid content. Expression of α-amylase in seeds soaked for 72 h at a temperature of 10°C was lower than expression of α-amylase when soaked at 25°C for the same amount of time. We observed variation in the expression of heat-resistant proteins Gene expression related to temperature tolerance in maize in seeds of the lines evaluated. The genes AOX and Zm-AN13 were promising for use in identifying maize materials that are tolerant to low germination temperatures.

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“…The annealing temperature of the tested primers is 60 °C and 53 °C for the reference gene ( Table S1 ). Relative quantification of gene expression and statistical analysis of all qRT-PCR data (pairwise fixed reallocation randomization test) were performed using the REST software according to Pfaffl et al 71 Actin 11 gene was used as a housekeeping reference gene 72 …”

Section: Methodsmentioning

confidence: 99%

Developmental stage- and concentration-specific sodium nitroprusside application results in nitrate reductase regulation and the modification of nitrate metabolism in leaves ofMedicago truncatulaplants

Antoniou

Filippou

Mylona³

et al. 2013

Plant Signaling & Behavior

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Nitric oxide (NO) is a bioactive molecule involved in numerous biological events that has been reported to display both pro-oxidant and antioxidant properties in plants. Several reports exist which demonstrate the protective action of sodium nitroprusside (SNP), a widely used NO donor, which acts as a signal molecule in plants responsible for the expression regulation of many antioxidant enzymes. This study attempts to provide a novel insight into the effect of application of low (100 μΜ) and high (2.5 mM) concentrations of SNP on the nitrosative status and nitrate metabolism of mature (40 d) and senescing (65 d) Medicago truncatula plants. Higher concentrations of SNP resulted in increased NO content, cellular damage levels and reactive oxygen species (ROS) concentration, further induced in older tissues. Senescing M. truncatula plants demonstrated greater sensitivity to SNP-induced oxidative and nitrosative damage, suggesting a developmental stage-dependent suppression in the plant’s capacity to cope with free oxygen and nitrogen radicals. In addition, measurements of the activity of nitrate reductase (NR), a key enzyme involved in the generation of NO in plants, indicated a differential regulation in a dose and time-dependent manner. Furthermore, expression levels of NO-responsive genes (NR, nitrate/nitrite transporters) involved in nitrogen assimilation and NO production revealed significant induction of NR and nitrate transporter during long-term 2.5 mM SNP application in mature plants and overall gene suppression in senescing plants, supporting the differential nitrosative response of M. truncatula plants treated with different concentrations of SNP.

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Alternative oxidase 1 (Aox1) gene expression in roots of Medicago truncatula is a genotype-specific component of salt stress tolerance

Cited by 46 publications

References 26 publications

Salinity tolerance is related to cyanide‐resistant alternative respiration in Medicago truncatula under sudden severe stress

Salinity tolerance is related to cyanide‐resistant alternative respiration in Medicago truncatula under sudden severe stress

Expression of genes related to tolerance to low temperature for maize seed germination

Developmental stage- and concentration-specific sodium nitroprusside application results in nitrate reductase regulation and the modification of nitrate metabolism in leaves ofMedicago truncatulaplants

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