Alternative Neural Crest Cell Fates Are Instructively Promoted by TGFβ Superfamily Members

Shah, Nirao M.; Groves, Andrew K.; Anderson, David J.

doi:10.1016/s0092-8674(00)81112-5

Cited by 740 publications

(637 citation statements)

References 70 publications

(30 reference statements)

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“…Although TGFb1 induces a smooth muscle cell fate in NCSCs on prolonged incubation [21], smooth muscle actin (SMA)-expressing smooth muscle cells were not present after 4 hours of TGFb1 priming (data not shown). Furthermore, short-term TGFb1 treatment did not affect cell survival (data not shown).…”

Section: Generation Of Mesenchymal Progenitors From Ncsc Cultures By mentioning

confidence: 96%

Transforming Growth Factor β-Mediated Sox10 Suppression Controls Mesenchymal Progenitor Generation in Neural Crest Stem Cells

et al. 2011

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During vertebrate development, neural crest stem cells (NCSCs) give rise to neural cells of the peripheral nervous system and to a variety of mesenchymal cell types, including smooth muscle, craniofacial chondrocytes, and osteocytes. Consistently, mesenchymal stem cells (MSCs) have recently been shown to derive in part from the neural crest (NC), although the mechanisms underlying MSC generation remains to be identified. Here, we show that transforming growth factor b (TGFb)-mediated suppression of the NCSC transcription factor Sox10 induces a switch in neural to mesenchymal potential in NCSCs. In vitro and in vivo, TGFb signal inactivation results in persistent Sox10 expression, decreased cell cycle exit, and perturbed generation of mesenchymal derivatives, which eventually leads to defective morphogenesis. In contrast, TGFb-mediated downregulation of Sox10 or its genetic inactivation suppresses neural potential, confers mesenchymal potential to NC cells in vitro, and promotes cell cycle exit and precocious mesenchymal differentiation in vivo. Thus, negative regulation of Sox10 by TGFb signaling promotes the generation of mesenchymal progenitors from NCSCs. Our study might lay the grounds for future applications demanding defined populations of MSCs for regenerative medicine. STEM CELLS 2011;29:689-699 Disclosure of potential conflicts of interest is found at the end of this article.

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Section: Generation Of Mesenchymal Progenitors From Ncsc Cultures By mentioning

confidence: 96%

Transforming Growth Factor β-Mediated Sox10 Suppression Controls Mesenchymal Progenitor Generation in Neural Crest Stem Cells

et al. 2011

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“…29 Cultured neural crest cells differentiate towards spindle-shaped SMCs when treated with TGF-β. 30 Moreover, TGF-β1 signalling has recently been shown to be required for SMCspecific gene expression in embryoid bodies. 31 In contrast to PDGF, TGF-β isoforms clearly promote the contractile phenotype in adult SMCs.…”

Section: Biochemical Factorsmentioning

confidence: 99%

Regulation and characteristics of vascular smooth muscle cell phenotypic diversity

Rensen¹,

Doevendans²,

Eys³

2007

NHJL

732

674

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Vascular smooth muscle cells can perform both contractile and synthetic functions, which are associated with and characterised by changes in morphology, proliferation and migration rates, and the expression of different marker proteins. The resulting phenotypic diversity of smooth muscle cells appears to be a function of innate genetic programmes and environmental cues, which include biochemical factors, extracellular matrix components, and physical factors such as stretch and shear stress. Because of the diversity among smooth muscle cells, blood vessels attain the flexibility that is necessary to perform efficiently under different physiological and pathological conditions. In this review, we discuss recent literature demonstrating the extent and nature of smooth muscle cell diversity in the vascular wall and address the factors that affect smooth muscle cell phenotype. (Neth Heart J 2007;15:100-8.)

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“…Wnt signaling on its own instructively promotes sensory neurogenesis in eNCSCs [19,20]. BMP signaling alone, on the other hand, instructively drives eNCSCs into the autonomic lineage in vitro [21], while in vivo BMP signaling regulates proliferation, differentiation and survival of NC-derived cells in a lineage specific fashion [22][23][24]. Cells with NCSC-like features can also be found in various postmigratory target structures of the neural crest, like in the embryonic sciatic nerve [25], adult skin [26,27], gut [28], adult dorsal root ganglia (DRG), whisker pad, and bone marrow [29].…”

Section: Stage-specific Control Of Stem Cells In the Pnsmentioning

confidence: 99%

Stage- and area-specific control of stem cells in the developing nervous system

Falk¹,

Sommer²

2009

Current Opinion in Genetics & Development

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The spatiotemporal control of proliferation and differentiation in neural stem cells (NSCs) is essential to produce a functional nervous system (NS). Stem cells in different areas and at different time points during development have to produce different types of cells in a precise manner. Recent studies uncovered a plethora of cell extrinsic as well as intrinsic factors that play crucial roles in the area-specific and stage-specific control of NSCs. Moreover, recapitulation of the spatiotemporal specification of NSCs in vitro opens new avenues for future applications. In this review, we have selected some key molecules to discuss the mechanisms underlying the spatiotemporal regulation of NSC development. Stage-and Area-Specific Control of Stem Cells in the Developing Nervous SystemSven Falk 1 , Lukas Sommer Summary of recent advancesThe spatiotemporal control of proliferation and differentiation in neural stem cells ( In this review, we have selected some key molecules to discuss the mechanisms underlying the spatiotemporal regulation of NSC development.

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Alternative Neural Crest Cell Fates Are Instructively Promoted by TGFβ Superfamily Members

Cited by 740 publications

References 70 publications

Transforming Growth Factor β-Mediated Sox10 Suppression Controls Mesenchymal Progenitor Generation in Neural Crest Stem Cells

Transforming Growth Factor β-Mediated Sox10 Suppression Controls Mesenchymal Progenitor Generation in Neural Crest Stem Cells

Regulation and characteristics of vascular smooth muscle cell phenotypic diversity

Stage- and area-specific control of stem cells in the developing nervous system

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