Alternative method in Galleria mellonella larvae to study biofilm infection and treatment

Campos-Silva, Rodrigo; Brust, Flávia Roberta; Trentin, Danielle da Silva; Macêdo, Alexandre José

doi:10.1016/j.micpath.2019.103756

Cited by 14 publications

(7 citation statements)

References 18 publications

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“…The G. mellonella infection model has been tested recently for this purpose (Maslova et al, 2020) Recently, the study of bacterial biofilm infections was successfully adapted in vivo in the G. mellonella model especially with S. aureus. Two techniques were employed to simulate S. aureus infection on indwelling devices: toothbrush bristles insertion and stainless steel or titanium implants (Campos- Silva et al, 2019;Mannala et al, 2021). First, abiotic surface insertions did not result in excess larval mortality, and secondly, by scanning electron microscopy, it was possible to observe S. aureus biofilm on indwelling devices and to determine the main steps of biofilm genesis such as attachment, proliferation and detachment.…”

Section: Innovating By Implementation Of Novel Infection Modelsmentioning

confidence: 99%

Galleria mellonella as a Suitable Model of Bacterial Infection: Past, Present and Future

Ménard

Rouillon

Cattoir

et al. 2021

Front. Cell. Infect. Microbiol.

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The increasing interest for Galleria mellonella larvae as an infection model is evidenced by the number of papers reporting its use, which increases exponentially since the early 2010s. This popularity was initially linked to limitation of conventional animal models due to financial, technical and ethical aspects. In comparison, alternative models (e.g. models using Caenorhabditis elegans, Drosophila melanogaster or G. mellonella) were cheap, simple to use and not limited by ethical regulation. Since then, similar results have been established with G. mellonella model comparatively to vertebrates, and it is more and more often used as a robust model per se, not only as an alternative to the murine model. This review attempts to summarize the current knowledge supporting the development of this model, both on immunological and microbiological aspects. For that, we focus on investigation of virulence and new therapies for the most important pathogenic bacteria. We also discuss points out directions for standardization, as well as recent advances and new perspectives for monitoring host-pathogen interactions.

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Section: Innovating By Implementation Of Novel Infection Modelsmentioning

confidence: 99%

Galleria mellonella as a Suitable Model of Bacterial Infection: Past, Present and Future

Ménard

Rouillon

Cattoir

et al. 2021

Front. Cell. Infect. Microbiol.

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“…In this study, we used the larvae of the wax moth G. mellonella as an in vivo model, for its well-known advantages as an alternative to vertebrate models. Although G. mellonella does not replace the vertebrate model, it can be exploited as a screening step between in vitro and in vivo evaluations [16].…”

Section: Discussionmentioning

confidence: 99%

“…In particular, G. mellonella is frequently exploited as an alternative to the murine model for studying microbial infections, because it is a simple, cheap and fast method, and implies fewer ethical concerns compared to the use of vertebrate models. As a matter of fact, several other studies have recently used G. mellonella to investigate the in vivo activity of antimicrobial agents against pathogenic microorganisms, including bacteria and fungi [16].…”

Section: Introductionmentioning

confidence: 99%

The Membranotropic Peptide gH625 to Combat Mixed Candida albicans/Klebsiella pneumoniae Biofilm: Correlation between In Vitro Anti-Biofilm Activity and In Vivo Antimicrobial Protection

Maione

Alteriis

Carraturo

et al. 2021

JoF

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The antibiofilm activity of a gH625 analogue was investigated to determine the in vitro inhibition and eradication of a dual-species biofilm of Candida albicans and Klebsiella pneumoniae, two leading opportunistic pathogens responsible for several resistant infections. The possibility of effectively exploiting this peptide as an alternative anti-biofilm strategy in vivo was assessed by the investigation of its efficacy on the Galleria mellonella larvae model. Results on larvae survival demonstrate a prophylactic efficacy of the peptide towards the infection of each single microorganism but mainly towards the co-infection. The expression of biofilm-related genes in vivo showed a possible synergy in virulence when these two species co-exist in the host, which was effectively prevented by the peptide. These findings provide novel insights into the treatment of medically relevant bacterial–fungal interaction.

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“…G. mellonella has been previously used to evaluate biofilm-related infections with a foreign material using different tooth brush bristles (Benthall et al, 2015;Campos-Silva et al, 2019). How-…”

Section: Discussionmentioning

confidence: 99%

“…Earlier, G. mellonella also was used to assess accumulation of bacterial pathogens on different toothbrush bristles to mimic biofilm infections on a foreign body. After piercing the larva proleg with the bristles, the signs of infection were observed, bacteria enumerated and biofilm analyzed by SEM (Benthall et al, 2015;Campos-Silva et al, 2019).…”

Section: Micro-ct Analysismentioning

confidence: 99%

Galleria mellonella as an alternative in vivo model to study bacterial biofilms on stainless steel and titanium implants_suppl2

Mannala

2020

ALTEX

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after eradication of the infection is high, both for the individual patient and with respect to costs (Zimmerli et al., 2004).Several in vitro and in vivo models have been developed to study the process of biofilm formation (Lebeaux et al., 2013). In translational research, in vivo models such as rat, rabbit, dog and sheep are widely used to assess the biocompatibility of orthopedic implants as well as to test antimicrobial coatings and compounds against biofilm formation caused by various microorganisms.In general, vertebrate models are restricted in use for scientific purposes due to animal welfare and ethical reasons. This is particularly true for infection experiments such as to study biofilm formation and treatment, as inoculation of bacteria or other microorganisms often results in a high burden of disease in the animals (Moriarty et al., 2019). Therefore, ethical approval is usually restricted. To provide best possible protection of re-

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Alternative method in Galleria mellonella larvae to study biofilm infection and treatment

Cited by 14 publications

References 18 publications

Galleria mellonella as a Suitable Model of Bacterial Infection: Past, Present and Future

Galleria mellonella as a Suitable Model of Bacterial Infection: Past, Present and Future

The Membranotropic Peptide gH625 to Combat Mixed Candida albicans/Klebsiella pneumoniae Biofilm: Correlation between In Vitro Anti-Biofilm Activity and In Vivo Antimicrobial Protection

Galleria mellonella as an alternative in vivo model to study bacterial biofilms on stainless steel and titanium implants_suppl2

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