Alternative Forms of Y-Box Binding Protein 1 and YB-1 mRNA

Lyabin, Dmitry N.; Doronin, A. N.; Eliseeva, Irina A.; Генс, Г. П.; Kulakovskiy, Ivan V.; Ovchinnikov, Lev P.

doi:10.1371/journal.pone.0104513

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…However, 5 of the 10 most abundant axonal TF mRNAs were significantly enriched in this compartment compared to somas, including Ybx1 , Carhsp1 , Pbx4 , Hmga1 , and Zfp580 (Figure 3C). The enrichment of Ybx1 (Y-box binding protein 1, p < 0.001) (Figure 3C) was particularly compelling since Ybx1 partakes in, e.g., pre-mRNA transcription and splicing, mRNA packaging, and regulation of mRNA stability and translation (Lyabin et al., 2014a, Lyabin et al., 2014b). RNAscope confirmed the presence of Ybx1 mRNA in Hb9-GFP+ motor axons (Figure 3D).…”

Section: Resultsmentioning

confidence: 99%

Axon-Seq Decodes the Motor Axon Transcriptome and Its Modulation in Response to ALS

et al. 2018

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SummarySpinal motor axons traverse large distances to innervate target muscles, thus requiring local control of cellular events for proper functioning. To interrogate axon-specific processes we developed Axon-seq, a refined method incorporating microfluidics, RNA sequencing (RNA-seq), and bioinformatic quality control. We show that the axonal transcriptome is distinct from that of somas and contains fewer genes. We identified 3,500–5,000 transcripts in mouse and human stem cell-derived spinal motor axons, most of which are required for oxidative energy production and ribogenesis. Axons contained transcription factor mRNAs, e.g., Ybx1, with implications for local functions. As motor axons degenerate in amyotrophic lateral sclerosis (ALS), we investigated their response to the SOD1G93A mutation, identifying 121 ALS-dysregulated transcripts. Several of these are implicated in axonal function, including Nrp1, Dbn1, and Nek1, a known ALS-causing gene. In conclusion, Axon-seq provides an improved method for RNA-seq of axons, increasing our understanding of peripheral axon biology and identifying therapeutic targets in motor neuron disease.

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Section: Resultsmentioning

confidence: 99%

Axon-Seq Decodes the Motor Axon Transcriptome and Its Modulation in Response to ALS

et al. 2018

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“…3C) was particularly compelling since Ybx1 partakes in e.g. pre-mRNA transcription and splicing, mRNA packaging and regulation of mRNA stability and translation (Lyabin et al, 2014a;Lyabin et al, 2014b).…”

Section: Motor Axons Show a Unique Transcription Factor Profilementioning

confidence: 99%

Axon-seq decodes the motor axon transcriptome and its modulation in response to ALS

Nijssen

Benitez

Hoogstraaten

et al. 2018

Preprint

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Spinal motor axons traverse large distances to innervate target muscles, thus requiring local control of cellular events for proper functioning. To interrogate axon-specific processes we developed Axon-seq, a refined method incorporating microfluidics, RNA-seq and bioinformatic-QC. We show that the axonal transcriptome is distinct from somas and contains fewer genes. We identified 3,500-5,000 transcripts in mouse and human stem cell-derived spinal motor axons, most of which are required for oxidative energy production and ribogenesis. Axons contained transcription factor mRNAs, e.g. Ybx1, with implications for local functions. As motor axons degenerate in amyotrophic lateral sclerosis (ALS), we investigated their response to the SOD1 G93A mutation, identifying 121 ALS-dysregulated transcripts. Several of these are implicated in axonal function, including Nrp1, Dbn1 and Nek1, a known ALS-causing gene. In conclusion, Axon-seq provides an improved method for RNA-seq of axons, increasing our understanding of peripheral axon biology and identifying novel therapeutic targets in motor neuron disease.

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“…These classes of proteins are identified by their ability to regulate the gene expression by binding to the Y-box promotor region [3]. Many reports have highlighted the role of YBX-1 in the regulation of vital processes including DNA replication [4], repair [5] and transcription [6] as well as mRNA splicing [7] and translation [8]. YBX-1 was also found to be dysregulated in many types of cancer including cervical [9], ovarian [10], gastric [11] and colorectal [12].…”

Section: Introductionmentioning

confidence: 99%

Serum Y-Box Binding Protein 1 (YBX-1) and Interleukin 6 (IL-6) Are Associated with Metastasis in Breast Cancer Patients

Abd-Elaziz¹,

Moneim²,

Fek³

et al. 2019

ABCR

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Objectives: The aim of this study was to assess the levels of Y-box binding protein 1 (YBX-1) and interleukin 6 (IL-6) in the sera of metastatic and non-metastatic breast cancer patients (BC), investigate their clinicopathological significance and to analyze their potential use as biomarkers of breast cancer metastasis. Methods: The study included ninety subjects sub-grouped equally into metastatic BC, non-metastatic BC and healthy volunteers. Serum YBX-1 and IL-6 were quantified using ELISA technique while CA 15-3 was quantified using IRMA kit. Clinical data were collected from patients' records. Results: YBX-1 (p < 0.001), IL-6 (p < 0.001) and CA15-3 (p = 0.017, 0.001) were significantly elevated in metastatic and non-metastatic BC patients compared to healthy controls, however, only YBX-1 (p < 0.001) and IL-6 (p < 0.001) showed a significant difference with cancer metastasis. Generally, YBX-1 and IL-6 were correlated with worse histological grade and late clinical stage in breast cancer patients and they were also associated with axillary lymph nodes involvement and positive vascular invasion in metastatic BC patients. Serum YBX-1 and IL-6 levels were positively correlated to each other (r s = 0.615, p < 0.001) and they showed high sensitivity and specificity compared to CA 15-3 (p < 0.001 and p = 0.004 for YBX-1 and IL-6 respectively) for predicting cancer metastasis. Conclusions: Serum YBX-1 and IL-6 are potential biomarkers of breast cancer patients with significant correlation with bad clinicopathological characteristics. Serum YBX-1 and IL-6 have superior sensitivity and specificity compared to CA15-3 and can serve as potential follow up and prognostic markers.

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Alternative Forms of Y-Box Binding Protein 1 and YB-1 mRNA

Cited by 6 publications

References 22 publications

Axon-Seq Decodes the Motor Axon Transcriptome and Its Modulation in Response to ALS

Axon-Seq Decodes the Motor Axon Transcriptome and Its Modulation in Response to ALS

Axon-seq decodes the motor axon transcriptome and its modulation in response to ALS

Serum Y-Box Binding Protein 1 (YBX-1) and Interleukin 6 (IL-6) Are Associated with Metastasis in Breast Cancer Patients

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