Alternative exon‐encoding regions of <i>Locusta migratoria</i> muscle myosin modulate the pH dependence of ATPase activity

Li, J.; Lu, Zhenwu; He, Jing; Chen, Qianquan; Wang, X.; Kang, Le; Li, X.-d.

doi:10.1111/imb.12254

Cited by 8 publications

(17 citation statements)

References 41 publications

(58 reference statements)

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“…blastn analysis of outlier loci found within clusters 845 and 632 revealed that the haplotype of 230 bp containing SNP 100240_75 (cluster 845/chromosome 7) exhibits high similarity with a repeat region from Locusta migratoria that correspond to an RTE‐2 retrotransposon (Ruiz‐Ruano et al, 2018) (Table ). Less stringent blastn searches and blastx analysis retrieved homology with myosin and troponin muscle genes (Li et al, 2016; Liang et al, 2020) and with a sulphotransferase enzyme (Chen et al, 2021), all from L . migratoria (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…In this case, S7). Less stringent blastn searches and blastx analysis retrieved homology with myosin and troponin muscle genes (Li et al, 2016;Liang et al, 2020) and with a sulphotransferase enzyme (Chen et al, 2021), all from L. migratoria (Table S7). Outlier loci from cluster 632 did not match with any known sequence.…”

Section: Outlier Loci and Correlation With Environmental Variablesmentioning

confidence: 99%

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Stable inversion clines in a grasshopper species group despite complex geographical history

et al. 2021

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Chromosomal inversions are known to play roles in adaptation and differentiation in many species. They involve clusters of correlated genes (i.e., loci in linkage disequilibrium, LD) possibly associated with environmental variables. The grasshopper “species complex” Trimerotropis pallidipennis comprises several genetic lineages distributed from North to South America in arid and semi‐arid high‐altitude environments. The southernmost lineage, Trimerotropis sp., segregates for four to seven putative inversions that display clinal variation, possibly through adaptation to temperate environments. We analysed chromosomal, mitochondrial and genome‐wide single nucleotide polymorphism data in 19 Trimerotropis sp. populations mainly distributed along two altitudinal gradients (MS and Ju). Populations across Argentina comprise two main chromosomally and genetically differentiated lineages: one distributed across the southernmost border of the “Andes Centrales,” adding evidence for a differentiation hotspot in this area; and the other widely distributed in Argentina. Within the latter, network analytical approaches to LD found three clusters of correlated loci (LD‐clusters), with inversion karyotypes explaining >79% of the genetic variation. Outlier loci associated with environmental variables mapped to two of these LD‐clusters. Furthermore, despite the complex geographical history indicated by population genetic analyses, the clines in inversion karyotypes have remained stable for more than 20 generations, implicating their role in adaptation and differentiation within this lineage. We hypothesize that these clines could be the consequence of a coupling between extrinsic postzygotic barriers and spatially varying selection along environmental gradients resulting in a hybrid zone. These results provide a framework for future investigations about candidate genes implicated in rapid adaptation to new environments.

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Section: Resultsmentioning

confidence: 99%

Section: Outlier Loci and Correlation With Environmental Variablesmentioning

confidence: 99%

Stable inversion clines in a grasshopper species group despite complex geographical history

et al. 2021

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“…The migratory locust, Locusta migratoria, contains a single Mhc gene comprised of 41 exons, including 5 clusters of alternative exclusive exons and 1 differently included penultimate exon (Fig. 1A) (Li et al, 2016). In the motor domain of locust MHC (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…In the tail of locust MHC, the S2 hinge region is encoded by 2 exon-30 variants (30a and 30b) and 2 types of tail tip can be produced by splicing in/out of exon-40. Theoretically, 360 different locust MHC isoforms can be generated by alternative-splicing exons (Li et al, 2016). Remarkably, the regions encoded by the alternative exons of locust Mhc gene are identical to those of Drosophila Mhc gene, indicating that both species use a similar strategy to modulate the motor functions of striated muscle myosin.…”

Section: Introductionmentioning

confidence: 99%

Alternative relay regulates the adenosine triphosphatase activity of Locusta migratoria striated muscle myosin

Hao,

Liu,

Zhang

et al. 2023

Insect Science

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Locust (Locusta migratoria) has a single striated muscle myosin heavy chain (Mhc) gene, which contains 5 clusters of alternative exclusive exons and 1 differently included penultimate exon. The alternative exons of Mhc gene encode 4 distinct regions in the myosin motor domain, that is, the N‐terminal SH3‐like domain, one lip of the nucleotide‐binding pocket, the relay, and the converter. Here, we investigated the role of the alternative regions on the motor function of locust muscle myosin. Using Sf9‐baculovirus protein expression system, we expressed and purified 5 isoforms of the locust muscle myosin heavy meromyosin (HMM), including the major isoform in the thorax dorsal longitudinal flight muscle (FL1) and 4 isoforms expressed in the abdominal intersegmental muscle (AB1 to AB4). Among these 5 HMMs, FL1‐HMM displayed the highest level of actin‐activated adenosine triphosphatase (ATPase) activity (hereafter referred as ATPase activity). To identify the alternative region(s) responsible for the elevated ATPase activity of FL1‐HMM, we produced a number of chimeras of FL1‐HMM and AB4‐HMM. Substitution with the relay of AB4‐HMM (encoded by exon‐14c) substantially decreased the ATPase activity of FL1‐HMM, and conversely, the relay of FL1‐HMM (encoded by exon‐14a) enhanced the ATPase activity of AB4‐HMM. Mutagenesis showed that the exon‐14a‐encoded residues Gly474 and Asn509 are responsible for the elevated ATPase activity of FL1‐HMM. Those results indicate that the alternative relay encoded by exon‐14a/c play a key role in regulating the ATPase activity of FL1‐HMM and AB4‐HMM.

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“…Insect flight muscle is generally not believed to express multiple fiber types that differ in MyHC isoform composition (Ayme-Southgate et al, 2015;Hooper and Thuma, 2005) and contractile properties. However, two recent studies suggest that multiple Myhc mRNAs can be expressed in Manduca sexta and Locusta migratoria flight muscle tissue (Ayme-Southgate et al, 2015;Li et al, 2016). Although it is not known whether these mRNAs are translated into functional MyHC protein isoforms for M. sexta, Ziegler et al (1994) reported the presence of two distinct MyHC protein isoforms in flight muscle of L. migratoria (and the fly Phormia terraenovae).…”

Section: Introductionmentioning

confidence: 99%

Parasitic gut infection causes functional and molecular resemblance of Libellula pulchella dragonfly flight muscle to skeletal muscle of obese vertebrates

Schilder

Stewart

2019

Journal of Experimental Biology

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We previously demonstrated the existence of a naturally occurring metabolic disease phenotype in Libellula pulchella dragonflies that shows high similarity to vertebrate obesity and type II diabetes, and is caused by a protozoan gut parasite. To further mechanistic understanding of how this metabolic disease phenotype affects fitness of male L. pulchella in vivo, we examined infection effects on in situ muscle performance and molecular traits relevant to dragonfly flight performance in nature. Importantly, these traits were previously shown to be affected in obese vertebrates. Similarly to obesity effects in rat skeletal muscle, dragonfly gut infection caused a disruption of relationships between body mass, flight muscle power output and alternative pre-mRNA splicing of troponin T, which affects muscle calcium sensitivity and performance in insects and vertebrates. In addition, when simulated in situ to contract at cycle frequencies ranging from 20 to 45 Hz, flight muscles of infected individuals displayed a left shift in power-cycle frequency curves, indicating a significant reduction in their optimal cycle frequency. Interestingly, these power-cycle curves were similar to those produced by flight muscles of non-infected teneral (i.e. physiologically immature) adult L. pulchella males. Overall, our results indicate that the effects of metabolic disease on skeletal muscle physiology in natural insect systems are similar to those observed in vertebrates maintained in laboratory settings. More generally, they indicate that study of natural, host-parasite interactions can contribute important insight into how environmental factors other than diet and exercise may contribute to the development of metabolic disease phenotypes.

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Alternative exon‐encoding regions of Locusta migratoria muscle myosin modulate the pH dependence of ATPase activity

Cited by 8 publications

References 41 publications

Stable inversion clines in a grasshopper species group despite complex geographical history

Stable inversion clines in a grasshopper species group despite complex geographical history

Alternative relay regulates the adenosine triphosphatase activity of Locusta migratoria striated muscle myosin

Parasitic gut infection causes functional and molecular resemblance of Libellula pulchella dragonfly flight muscle to skeletal muscle of obese vertebrates

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