Alternative Cutaneous Substitutes Based on Poly(l-co-d,l-lactic acid-co-trimethylene carbonate) with Schinus terebinthifolius Raddi Extract Designed for Skin Healing

Abstract

The search for new therapies and drugs that act as topical agents to relieve pain and control the inflammatory processes in burns always attracted interest in clinical trials. As an alternative to synthetic drugs, natural extracts are useful in the development of new strategies and formulations for improving the quality of life. The aim of this study was to develop a wound dressing using poly(l-co-d,l-lactic acid-co-trimethylene carbonate) (PLDLA-TMC) containing Schinus terebinthifolius Raddi (S.T.R.). S.T.R. is a native Brazilian plant known for its strong anti-inflammatory responses. The membrane of PLDLA-TMC + S. terebinthifolius Raddi was prepared at different concentrations of S.T.R. (5, 10, 15, and 50%). The Fourier transform infrared results showed no change in the PLDLA-TMC spectrum after S.T.R. addition, whereas the swelling test showed changes only in PLDLA-TMC + S.T.R. at 50%. The wettability measurements showed a mass loss due to the decrease in the contact angle in all samples after the S.T.R. addition in the polymer, whereas the S.T.R. release test showed a linear delivery pattern. The scanning electron microscopy analysis showed that S.T.R. was homogeneously distributed at only 5 and 10%. Tensile tests demonstrated an increase in Young’s modulus and a reduction in the elongation till rupture of PLDLA-TMC after the addition of S.T.R. The biocompatibility in vitro evaluation with rat fibroblast cells seeded in the membranes of PLDLA-TMC + S.T.R. showed that although S.T.R. interfered in cell morphology, all concentrations tested showed that cells were able to adhere and proliferate during 7 days. Thus, S.T.R. at 50% was chosen to be tested for in vivo trials. The histological and immunohistochemistry results revealed an accelerated skin healing at 7 days after controlled secondary burns were introduced in the dorsal skin, with a striking total recovery of the epidermis and high rates of molecular activation of cell proliferation. Due to the known biocompatibility properties of PLDLA-TMC and its stable release of S.T.R., we strongly recommend S.T.R.-containing PLDLA-TMC as a curative device to favor skin healing.

Poly(L-co-D,L lactic acid-co-Trimethylene Carbonate) 3D printed scaffold cultivated with mesenchymal stem cells directed to bone reconstruction: In vitro and in vivo studies

“…The osteoinductive properties is a characteristic that must be evoked in a material, and not only the biocompatibility itself such as adhesion and proliferation. Additionally, this new material (patent pending of our laboratory 21 ), can be processed in different forms to possess a variety of approaches (even for skin healing as recently published by Komatsu et al 7 In this work, the successful 3D printing process of PLDLA-TMC gives rise to assorted new designs of this material for bone formation, since one can modulate parameters of porosity and shape of the obtained device.…”

“…It was developed by our research group as a promising material, due capacity of PLDLA to its biological reabsorption abilities and biocompatibility. Previous studies in recent literature related to PLDLA-TMC based-materials in skin tissue regeneration 7,8 and tendon 9 demonstrated that the terpolymer is versatile in a wide range of medical applications, as well as presenting biocompatibility characteristics. Furthermore, the TMC shows flexibility and degradation rates consistent with the applications designed to bone tissue engineering.…”

New approaches to second-degree burn healing: Polyvinyl alcohol membrane loaded to arnica combined to laser therapy

“…In the results of immunohistochemistry with the specific molecular marker for the inflammatory response, CD68, capable of identifying macrophages, it was verified that in all treated groups (7 or 14 days) there was an expressive detection of positivity for macrophages (Table 5, p > .05 compared to control) corroborates the evolution in the tissue repair process, since the literature shows that poor macrophage response impairs healing and wound closure, as the potential participation of macrophages leads to fibrotic tissue and efficient tissue repair. [48][49][50] Unlike the results obtained by Komatsu et al 51 who demonstrated weak CD68 labeling in the dermal wound scab of treated rodents when treated with Schinus terebinthifolius Raddi extract.…”

In Vitro Cell Behavior and Antibiotic Activity under Sustained Release of Doxycycline-Loaded Poly(lactic-co-glycolic acid) Microspheres

“…All samples submitted to microscopy were cultured in Thermanox ® coverslips (Nunc Thermo Scientific, Rochester, NY, USA). Cell proliferation and viability were evaluated after 1 and 3 days, as described by Komatsu et al (2019) [20]. Cells were subjected to fluorescence live imaging (Live/Dead KIT) and analyzed by laser scanning confocal microscopy (LSCM) (model TCS SP8; Leica Microsystems GmbH, Heidelberg, Germany).…”