Alternative chromophores for use in light-activated surgical adhesives

Byrd, Brian D; Heintzelman, Douglas L.; McNally-Heintzelman, Karen M.

doi:10.1117/12.476395

Cited by 4 publications

(6 citation statements)

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“…16 ICG and MB, on the other hand, showed a significant decrease in absorbance units with increased time and temperature when heated to temperatures up to 100 ˚C. Photobleaching was also observed in both ICG and MB solutions with exposure to a white light source.…”

Section: Discussionmentioning

confidence: 69%

“…16 Focus on these three alternative chromophores was based on the fact that they all had absorption peaks near the operating wavelength of existing medical lasers. The red food coloring had an absorption peak (λ = 500 nm) that would allow it to be used with coumarin dye, Cu Vapor, Ar Ion, and Kr Ion lasers.…”

Section: Discussionmentioning

confidence: 99%

“…16 The GFC had two absorption peaks at 417 nm and 630 nm, corresponding to the two dye components comprising this color. The lasers available for use in this study were a 5W 808nm diode laser (Spectra Physics, Mountain View, CA) for use with ICG, a 400mW 670nm diode laser (Coherent Inc., Santa Clara, CA) for use with MB, and a 35mW 632.8nm diode laser (Coherent Inc.) for use with BFC and GFC.…”

Section: Optical Penetration Depth Of Laser Light In Chromophore-enhamentioning

confidence: 99%

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Alternative chromophores for use in light-activated surgical adhesives: optimization of parameters for tensile strength and thermal damage profile

et al. 2003

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The use of indocyanine green-doped albumin protein solders has been shown to vastly improve the anastomotic strength that can be achieved by laser tissue repair techniques, while at the same time minimizing collateral thermal tissue damage. However, the safety of the degradation products of the chromophore following laser irradiation is uncertain. Therefore, we studied the feasibility of using alternative chromophores in terms of temperature rise at the solder/tissue interface, the extent of thermal damage in the surrounding tissue, and the tensile strength of repairs. Biodegradable polymer scaffolds of controlled porosity were fabricated with poly(L-lactic-co-glycolic acid), using a solvent-casting and particulate-leaching technique. The porous scaffold acted as a carrier to the traditional protein solder composition of serum albumin and an absorbing chromophore mixed in deionized water. Two commonly used chromophores, indocyanine green and methylene blue were investigated, as well as blue and green food colorings.Temperature rise at the solder surface and at the interface between the solder and tissue were monitored by an IR temperature monitoring system and a type-K thermocouple, respectively, and the extent of thermal damage in the underlying tissue was determined using light microscopy. As expected, temperature rise at the solder/tissue interface, and consequently the degree of collateral thermal tissue damage, was directly related to the penetration depth of the laser light in the protein solder. Optimal tensile strength of repairs was achieved by selecting a chromophore concentration that resulted in a temperature of 66 ± 3 °C at the solder/tissue interface.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Optical Penetration Depth Of Laser Light In Chromophore-enhamentioning

confidence: 99%

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Alternative chromophores for use in light-activated surgical adhesives: optimization of parameters for tensile strength and thermal damage profile

et al. 2003

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“…The interface reached 80 C after just 19.7 s. At this time the intima temperature increased to 71. 6 C. The complete results of this part of the parameter study can be seen in Table 3. Only four settings fulfil our constraints.…”

Section: Absorption Propertiesmentioning

confidence: 98%

“…A chromophore-enhanced protein solder is put onto the anastomosis site, which absorbs the laser energy [6,7]. In most cases, bovine serum albumin (BSA) is used.…”

Section: Biophotonicsmentioning

confidence: 99%

Thermal model for optimization of vascular laser tissue soldering

Bogni

Stumpp

Reinert

et al. 2010

Journal of Biophotonics

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Laser tissue soldering (LTS) is a promising technique for tissue fusion based on a heat-denaturation process of proteins. Thermal damage of the fused tissue during the laser procedure has always been an important and challenging problem. Particularly in LTS of arterial blood vessels strong heating of the endothelium should be avoided to minimize the risk of thrombosis. A precise knowledge of the temperature distribution within the vessel wall during laser irradiation is inevitable. The authors developed a finite element model (FEM) to simulate the temperature distribution within blood vessels during LTS. Temperature measurements were used to verify and calibrate the model. Different parameters such as laser power, solder absorption coefficient, thickness of the solder layer, cooling of the vessel and continuous vs. pulsed energy deposition were tested to elucidate their impact on the temperature distribution within the soldering joint in order to reduce the amount of further animal experiments. A pulsed irradiation with high laser power and high absorbing solder yields the best results.

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