This study examined aspects of reliability and validity of the bilateral coordination and motor sequencing items of the Assessment of Motor Coordination and Dexterity (AMCD) for Brazilian children ages 4, 6 and 8 years old. The AMCD aims to identify children with developmental coordination disorder (DCD). A total of 84 children were evaluated. Among the 35 items piloted, all but one presented interrater reliability above 0.80 and 16 (45.7%) items presented intraclass correlation coefficient over 0.60 for test-retest reliability. Most items were sensitive to age difference and only items involving ball handling exhibited significant gender differences. As a result, this section of the AMCD could be reduced to the 20 items that were found to be reliable and more discriminative for age-related differences. This study advances on the development of the AMCD, but a limitation was the noninclusion of children with DCD. Future research should investigate whether the selected items are useful in differentiating the motor skills of children with and without coordination problems.