Alternation of β-catenin and CD44s Immunoexpression in Different Histopathological Grades of Oral Squamous Cell Carcinoma

Zargaran, Massoumeh

doi:10.31557/apjcp.2020.21.5.1181

Cited by 2 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason to include β-catenin as a positive control for immunohistochemistry was due to its overexpression in OSCC tissues, as reviewed in. 17 For a negative control, the sections were incubated in the blocking serum without primary antibody. Subsequently, the sections were washed in PBS, and a 100µl volume of the secondary antibody (1:2000; Dako Real TM EnVision) in the antibody diluent was added and incubated for 30 min at 25℃.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Overexpression of methyltransferase‐like 3 and 14 in oral squamous cell carcinoma

Makeudom

Sun

et al. 2021

J Oral Pathology Medicine

View full text Add to dashboard Cite

Objectives: This study aimed to determine expressions of methyltransferase-like 3 (METTL3) and METTL14, two enzymes essential for mRNA methylation at the adenosine (m 6 A), in oral squamous cell carcinoma (OSCC) and to investigate in vitro aggressiveness of their aberrant expressions. Methods: METTL3 and METTL14 expressions in 50 OSCC and 11 normal oral tissues were examined by immunohistochemistry. METTL3 and METTL14 expressions and m 6 A amounts were determined in three OSCC cell lines, including HN5, HN6, and HN15. Cell proliferation, migration, and invasion were studied by BrdU, wound healing, and Transwell chamber assays, after silencing of METTL3, METTL14, or both by siRNA transfection. Results: Immunostaining of METTL3 and METTL14 was localized in cancer cell nuclei.The mean percentages of METTL3-and METTL14-positive cells were significantly increased in OSCC tissues (p < 0.001). The percentages of METTL3-and METTL14positive cells were correlated with the advanced pTNM stages (p < 0.05) and with the degrees of histopathological differentiation in OSCC (r = 0.564 and r = 0.316, respectively; p < 0.001). By the COX multivariate analysis, both overexpressed METTL3 and METTL14 were significantly associated with short overall survival (p < 0.05).Both METTL3 and METTL14 expressions and the m 6 A amounts were significantly increased in HN6 (p < 0.05). Silencing of METTL3 and METTL14 in HN6 significantly inhibited cell proliferation (p < 0.01), but it failed to mitigate cell migration or invasion.Conclusions: METTL3 and METTL14 are overexpressed in OSCC tissues and in the HN6 OSCC cell line that promotes cell proliferation. Overexpressed METTL3 or METTL14 is found to be an independent prognostic factor for short overall survival in patients with OSCC.

show abstract

Section: Immunohistochemistrymentioning

confidence: 99%

Overexpression of methyltransferase‐like 3 and 14 in oral squamous cell carcinoma

Makeudom

Sun

et al. 2021

J Oral Pathology Medicine

View full text Add to dashboard Cite

show abstract

“…It seems that more occurrences of abnormal β-catenin changes, higher rate of aberrant cytoplasmic expression, alongside with less membranous expression can significantly lead to lower degree of cellular differentiation in OSCC. (26) Concerning β-catenin expression, normal oral epithelial cells had the highest statistically significant mean area percent, followed by epithelial dysplasia; poorly-differentiated OSCC had the lowest mean area percent values. No statistically significant difference existed between oral epithelial dysplasia and OSCC groups, both are lower than normal.…”

Section: Discussionmentioning

confidence: 92%

Role of the Wnt signalling pathway in oral cancer

abdelsamia

2023

Egyptian Dental Journal

View full text Add to dashboard Cite

The sixth most dangerous cancer worldwide is oral cancer. Pre-cancer disorders are often present in many patients before oral cancer develops. Several types of human cancer have been linked to abnormal activation of Wnt/β-catenin signaling, which is characterized by β-catenin translocation and overexpression of Wnt ligands. β-catenin interacts with the transcription factor during canonical Wnt/β-catenin signaling to activate downstream target genes such as Cyclin D1, which is primarily associated with cellular proliferation. This study aims to evaluate the role of β-catenin and Cyclin D1 in epithelial dysplasia and various stages of oral squamous cell carcinoma (OSCC) via the Wnt signaling pathway. For the present study, 5 cases of normal oral epithelial tissues, 10 cases of severe epithelial dysplasia, and 30 cases of various grades of OSCC (10 cases for each grade) were collected as paraffin-embedded blocks. All specimens were immunohistochemically stained for β-catenin and Cyclin D1 antibodies. β-catenin immunostaining showed the highest mean area percentage found in normal epithelial tissue, whereas the lowest values were recorded in poorly-differentiated OSCC, while Cyclin D1 immunostaining showed the lowest mean area percent was recorded in normal epithelial tissue, whereas the highest values were noted in poorlydifferentiated OSCC. Conclusion: Aberrant cytoplasmic expression of β-catenin act as a co-factor, activating Cyclin D1 lead to high proliferative activity and invasion potential. Thus associations of both molecules has greater role in transformation from normal to dysplastic cells and increase their invasiveness to OSCC.

show abstract

Alternation of β-catenin and CD44s Immunoexpression in Different Histopathological Grades of Oral Squamous Cell Carcinoma

Cited by 2 publications

References 17 publications

Overexpression of methyltransferase‐like 3 and 14 in oral squamous cell carcinoma

Overexpression of methyltransferase‐like 3 and 14 in oral squamous cell carcinoma

Role of the Wnt signalling pathway in oral cancer

Contact Info

Product

Resources

About