Alternating current voltammetry of dopamine and ascorbic acid at carbon paste and stearic acid modified carbon paste electrodes

Gelbert, Mark B.; Curran, D. J.

doi:10.1021/ac00297a010

Cited by 98 publications

(19 citation statements)

References 14 publications

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“…A review of the historical and technical aspects of the field of in vivo electrochemistry is referenced (5). Electrocataiytic interactions between DA and ascorbic acid (AA) have been reported with a stearic acid macroelectrode in vitro (25), but more recent reports show that these interactions are insignificant in neuronal tissue in vivo when a stearic acid microelectrode is used (2). Precalibration and postcalibration procedures were done as previously described (6).…”

Section: In Vivo Electrochemical (Voltammetric) Biotechnologymentioning

confidence: 99%

Real time detection of acute (IP) cocaine-enhanced dopamine and serotonin release in ventrolateral nucleus accumbens of the behaving Norway rat

Broderick

Kornak

Eng

et al. 1993

Pharmacology Biochemistry and Behavior

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Cocaine (10 mg/kg), administered intraperitoneal (IP), was studied for its effects on dopamine (DA) and serotonin (5-HT) release in ventrolateral nucleus accumbens (vlNAcc) of conscious and behaving male, virus-free, Sprague-Dawley rats with in vivo electrochemistry (voltammetry). Miniature stearate probes detected DA and 5-HT release, on line and within a temporal resolution of seconds. Psychostimulant behaviors, in the form of four behavioral components (i.e., the classically DA-dependent behaviors of locomotor activity [ambulations], rearing, and stereotypy, and a 5-HT-ergic behavior, central ambulations) were studied concurrently with infrared photobeam detection. The results show that (IP) cocaine significantly increased vlNAcc DA release (p < 0.0001) and 5-HT release (p < 0.0012). Each of the four parameters of cocaine-induced psychostimulant behavior was concurrently and significantly increased as well (ambulations: p < 0.0001); rearing p < 0.0008; stereotypy: p < 0.0004; central ambulations: p < 0.0082). Moreover, exactly coincident data points for DA and 5-HT release occurred 10 and 40 min after (IP) cocaine administration. Cocaine-induced DA and 5-HT release were highly and positively correlated during the first hour of study (p < 0.01). As expected, increased DA release in vlNAcc after cocaine administration was significantly and positively correlated with classically DA-dependent behaviors (first- and second-hour effects) (p < 0.01) and with the 5-HT-ergic behavior, central ambulations (p < 0.01). Also, cocaine-induced 5-HT release was significantly and positively correlated with 5-HT behavior (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: In Vivo Electrochemical (Voltammetric) Biotechnologymentioning

confidence: 99%

Real time detection of acute (IP) cocaine-enhanced dopamine and serotonin release in ventrolateral nucleus accumbens of the behaving Norway rat

Broderick

Kornak

Eng

et al. 1993

Pharmacology Biochemistry and Behavior

View full text Add to dashboard Cite

show abstract

“…A review of the historic and technical aspects of the field of in vivo electrochemistry is referenced (6). Electrocatalytic interactions between DA and ascorbic acid (AA) have been reported with a stearic acid macroelectrode in vitro (21), but more recent reports show that these interactions are insignificant in neuronal tissue in vivo when a stearic acid microelectrode is used (2).…”

Section: In Vivo Electrochemical (Voltammetric) Biotechnologymentioning

confidence: 99%

Distinguishing effects of cocaine IV and SC on mesoaccumbens dopamine and serotonin release with chloral hydrate anesthesia

Broderick

1992

Pharmacology Biochemistry and Behavior

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The effect of i.v. cocaine (0.5 and 1.0 mg/kg) was studied on synaptic concentrations of dopamine (DA) and serotonin [5-hydroxytryptamine (5-HT)] in the mesoaccumbens nerve terminal, the nucleus accumbens (NAcc), in chloral hydrate-anesthetized, male Sprague-Dawley rats (Rattus norvegicus) with in vivo electrochemistry (voltammetry). In further in vivo voltammetric studies, the effects of SC cocaine on synaptic concentrations of DA and 5-HT were studied in the chloral hydrate-anesthetized paradigm in two neuroanatomic substrates, NAcc and mesoaccumbens somatodendrites, the ventral tegmental area (VTA-A10), in a dose-response fashion (10, 20, and 40 mg/kg) in six separate studies. Moreover, in two additional in vivo voltammetric studies, again using the chloral hydrate-anesthetized paradigm, the impulse flow blocker, gamma-butyrolactone (gamma-BL) (750 mg/kg, IP), was studied alone and in combination with SC cocaine (20 mg/kg) to determine whether or not cocaine can act by presynaptic releasing mechanisms for DA and 5-HT. The results show that IV cocaine concurrently and significantly increased DA and 5-HT release in the NAcc (p < 0.001, p < 0.0005, respectively) at both doses tested. Moreover, IV cocaine effects on DA and 5-HT release were significantly and positively correlated (p < 0.01). On the other hand, SC cocaine concurrently and significantly decreased DA and 5-HT release in NAcc (p < 0.0001) and VTA (p < 0.0001) at each separate dose tested. SC cocaine effects on DA and 5-HT release were significantly and positively correlated across dose and neuroanatomic substrate (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Recently, polymer films with high stability and selectivity have been widely used as coatings to detect biological substances. [9][10][11] In this paper, we describe the improved voltammetric behavior of uric acid and its trace determination at a polyglycine chemically modified electrode. The resolution of uric acid and ascorbic acid in a mixture is also reported.…”

mentioning

confidence: 99%

Catalytic Oxidation of Uric Acid at the Polyglycine Chemically Modified Electrode and its Trace Determination

Yu¹,

Zhang²,

Chen³

1997

Analyst

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Cyclic voltammetry was undertaken to investigate the electrochemical behavior of uric acid at a polyglycine modified electrode. The modified electrode shows catalytic ability for the oxidation of uric acid, reducing the overpotential by 250 mV in pH 7.0 phosphate buffer solution. The enhanced voltammetric response can be used to determine uric acid. The linear range is between 5.0 x 10(-8) and 4.5 x 10(-6) M with a detection limit as low as 5.0 x 10(-9) M. The relative standard deviation is 1.4% (8 runs) at a concentration of 50 microM uric acid. The catalytic effect of the modified electrode resulted in the voltammetric resolution of the overlapping of uric acid and ascorbic acid. This allows the simultaneous detection of uric acid and ascorbic acid in the same sample.

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Alternating current voltammetry of dopamine and ascorbic acid at carbon paste and stearic acid modified carbon paste electrodes

Cited by 98 publications

References 14 publications

Real time detection of acute (IP) cocaine-enhanced dopamine and serotonin release in ventrolateral nucleus accumbens of the behaving Norway rat

Real time detection of acute (IP) cocaine-enhanced dopamine and serotonin release in ventrolateral nucleus accumbens of the behaving Norway rat

Distinguishing effects of cocaine IV and SC on mesoaccumbens dopamine and serotonin release with chloral hydrate anesthesia

Catalytic Oxidation of Uric Acid at the Polyglycine Chemically Modified Electrode and its Trace Determination

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