Altering Sterol Composition Implied That Cholesterol Is Not Physiologically Associated With Diosgenin Biosynthesis in Trigonella foenum-graecum

Cao, Liyang; Zhou, Zilin; Sun, Jian; Li, Changfu; Zhang, Yansheng

doi:10.3389/fpls.2021.741604

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…Changes in the expression of genes, the stability of proteins, and the activity of enzymes in either of these two pathways can affect a wide range of biological processes in plants [18]. For example, it was found that treating Trigonella foenum-graecum with a speci c inhibitor of HMGR (3-hydroxy-3-methyl glutaryl coenzyme A reductase) for 72 hours signi cantly inhibited root growth and resulted in a approximately 50% decrease in diosgenin content [19]. Overexpression of MdHMGR5 (from Malus pumila) in Arabidopsis thaliana enhanced the antioxidant stress capacity of the plant [20].…”

Section: Introductionmentioning

confidence: 99%

Time-series transcriptome analysis reveals the cascade mechanism of biological processes after perturbation of the MVA pathway in Salvia miltiorrhiza

Liu,

Li,

Yan

et al. 2024

Preprint

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Background Various biological processes are interconnected in plants. Transcription factors (TFs) often act as regulatory hubs to regulate plant growth and responses to stress by integrating various biological pathways. Despite extensive studies on TFs functions in various plant species, our understanding of the details of TFs regulation remains limited. In this study, clonal seedlings of Salvia miltiorrhiza were exposed to specific inhibitors for 12 hours. Time-series transcriptome data, sampled hourly, were used to construct co-expression networks and gene regulatory networks (GRNs). Transcriptome dynamic analysis was utilized to capture the gene expression dynamics of various biological processes and decipher the potential molecular mechanisms that regulate these processes. Results The perturbation results showed the growth and development processes of S.miltiorrhiza were primarily affected at the early stage, whereas stress response-related biological processes were mainly influenced at the later stage. And there was a correlation between the series of key differentially expressed genes in terpenoid biosynthesis pathways and the topological distribution of these pathways. Furthermore, the GRNs based on TFs indicate that TFs play a crucial role in connecting various biological processes. In the cytoplasmic lysate gene regulatory module, SmWRKY48-SmTCP4-SmWRKY28 constituted a regulation hub regulating S.miltiorrhiza responses to perturbation of the MVA pathway. The regulation hub mediated various pathways, including pyruvate metabolism, glycolysis/gluconeogenesis, amino acid metabolism, and ubiquinone and other terpenoid-quinone biosynthesis. Conclusion Our findings suggest that perturbation of a key biological pathway in S.miltiorrhiza has time-dependent effects on other biological processes. And SmWRKY48-SmTCP4-SmWRKY28 constitutes the regulatory hub in S.miltiorrhiza responses to perturbation of MVA pathway.

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Section: Introductionmentioning

confidence: 99%

Time-series transcriptome analysis reveals the cascade mechanism of biological processes after perturbation of the MVA pathway in Salvia miltiorrhiza

Liu,

Li,

Yan

et al. 2024

Preprint

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3-Hydroxy-3-methylglutaryl coenzyme A reductase genes from Glycine max regulate plant growth and isoprenoid biosynthesis

Wang

Feng

Lou

et al. 2023

Sci Rep

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Isoprenoids, a large kind of plant natural products, are synthesized by the mevalonate (MVA) pathway in the cytoplasm and the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway in plastids. As one of the rate-limiting enzymes in the MVA pathway of soybean (Glycine max), 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) is encoded by eight isogenes (GmHMGR1–GmHMGR8). To begin, we used lovastatin (LOV), a specific inhibitor of GmHMGR, to investigate their role in soybean development. To further investigate, we overexpressed the GmHMGR4 and GmHMGR6 genes in Arabidopsis thaliana. The growth of soybean seedlings, especially the development of lateral roots, was inhibited after LOV treatment, accompanied by a decrease in sterols content and GmHMGR gene expression. After the overexpression of GmHMGR4 and GmHMGR6 in A. thaliana, the primary root length was higher than the wild type, and total sterol and squalene contents were significantly increased. In addition, we detected a significant increase in the product tocopherol from the MEP pathway. These results further support the fact that GmHMGR1–GmHMGR8 play a key role in soybean development and isoprenoid biosynthesis.

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Diosgenin biosynthesis pathway and its regulation in Dioscorea cirrhosa L.

Lin,

Hu,

et al. 2024

PeerJ

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Dioscorea cirrhosa L. (D. cirrhosa) tuber is a traditional medicinal plant that is abundant in various pharmacological substances. Although diosgenin is commonly found in many Dioscoreaceae plants, its presence in D. cirrhosa remained uncertain. To address this, HPLC-MS/MS analysis was conducted and 13 diosgenin metabolites were identified in D. cirrhosa tuber. Furthermore, we utilized transcriptome data to identify 21 key enzymes and 43 unigenes that are involved in diosgenin biosynthesis, leading to a proposed pathway for diosgenin biosynthesis in D. cirrhosa. A total of 3,365 unigenes belonging to 82 transcription factor (TF) families were annotated, including MYB, AP2/ERF, bZIP, bHLH, WRKY, NAC, C2H2, C3H, SNF2 and Aux/IAA. Correlation analysis revealed that 22 TFs are strongly associated with diosgenin biosynthesis genes (—r2— > 0.9, P < 0.05). Moreover, our analysis of the CYP450 gene family identified 206 CYP450 genes (CYP450s), with 40 being potential CYP450s. Gene phylogenetic analysis revealed that these CYP450s were associated with sterol C-22 hydroxylase, sterol-14-demethylase and amyrin oxidase in diosgenin biosynthesis. Our findings lay a foundation for future genetic engineering studies aimed at improving the biosynthesis of diosgenin compounds in plants.

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Altering Sterol Composition Implied That Cholesterol Is Not Physiologically Associated With Diosgenin Biosynthesis in Trigonella foenum-graecum

Cited by 3 publications

References 41 publications

Time-series transcriptome analysis reveals the cascade mechanism of biological processes after perturbation of the MVA pathway in Salvia miltiorrhiza

Time-series transcriptome analysis reveals the cascade mechanism of biological processes after perturbation of the MVA pathway in Salvia miltiorrhiza

3-Hydroxy-3-methylglutaryl coenzyme A reductase genes from Glycine max regulate plant growth and isoprenoid biosynthesis

Diosgenin biosynthesis pathway and its regulation in Dioscorea cirrhosa L.

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