Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov.

Abstract

A novel marine bacterium, strain JCS350T, was isolated from marine sediment samples collected from a cold-seep area. The 16S rRNA gene sequence of the isolate showed high similarity to that of Erythrobacter luteolus SW-109T (95.9 % sequence similarity). Lower 16S rRNA gene sequence similarities were shown to other members of the genus Erythrobacter (94.6–95.4 %) and members of the genus Porphyrobacter (94.5–95.2 %). Phylogenetic analysis with all members of the family Erythrobacteraceae and several members of the family Sphingomonadaceae revealed that the isolate formed a phyletic line with [Erythrobacter] luteolus that was distinct from other members of the family Erythrobacteraceae. The dominant fatty acids of strain JCS350T were 18 : 1ω7c, 16 : 1ω7c and cyclopropane 17 : 0. The major respiratory quinone was ubiquinone 10. The DNA G+C content was 54.5 mol%. The isolate did not contain bacteriochlorophyll a. Optimal growth required the presence of 2 % (w/v) NaCl with either 0.18 % CaCl2 or 0.59 % MgCl2, at pH 6.5 and at 35 °C. On the basis of the evidence of this polyphasic taxonomic study, strain JCS350T should be classified in a novel genus and species in the family Erythrobacteraceae, for which the name Altererythrobacter epoxidivorans gen. nov., sp. nov. is proposed. The misclassified species [Erythrobacter] luteolus is transferred to the new genus as Altererythrobacter luteolus comb. nov. The type strain of Altererythrobacter epoxidivorans is JCS350T (=KCCM 42314T =JCM 13815T) and the type strain of Altererythrobacter luteolus is SW-109T (=KCTC 12311T =JCM 12599T).

Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae

“…Table 1. Phenotypic characteristics that differentiate strain E4A9 T from other related members of the family Erythrobacteraceae Strains/taxa: 1, strain E4A9 T ; 2, Altererythrobacter (Yoon et al, 2005b;Kwon et al, 2007); 3, Erythrobacter (Shiba & Simidu, 1982;Yurkov et al, 1994;Denner et al, 2002;Yoon et al, 2003Yoon et al, , 2004aYoon et al, , 2005aIvanova et al, 2005); 4, Erythromicrobium (Yurkov et al, 1994;Rainey et al, 2003); 5, Porphyrobacter (Fuerst et al, 1993;Hanada et al, 1997;Hiraishi et al, 2002;Rainey et al, 2003;Yoon et al, 2004bYoon et al, , 2006. Data refer to all species within the genera.…”

“…Ubiquinone-10 is the major respiratory quinone. Most species in the family have been isolated from various aquatic environments such as freshwater, seawater, marine mats or sediment, a hot spring, seaweed, a starfish and coral (Shiba & Simidu, 1982; Fuerst et al, 1993;Yurkov et al, 1994; Hanada et al, 1997; Denner et al, 2002; Hiraishi et al, 2002;Rainey et al, 2003;Yoon et al, 2003Yoon et al, , 2004a Yoon et al, , b, 2005a Yoon et al, , b, 2006Ivanova et al, 2005;Kwon et al, 2007). This study focuses on the description of strain E4A9 T , isolated from a deep-sea sediment sample.…”

“…The family Erythrobacteraceae (order Sphingomonadales, class Alphaproteobacteria) was proposed by Lee et al (2005) based on a comprehensive phylogenetic analysis and, at present, it comprises four recognized genera: Altererythrobacter (Kwon et al, 2007), Erythrobacter (Shiba & Simidu, 1982), Erythromicrobium (Yurkov et al, 1994) and Porphyrobacter (Fuerst et al, 1993).…”

In silico analysis of cysteine content, in six genes, across three marine Altererythrobacter strains sampled at varying barometric pressures

“…A. atlanticus was sampled (at 2500 m depth) and genomically annotated by Wu et al (2015). A. epoxidivorans was sampled (at a depth of 120 m) and genomically annotated by Kwon et al (2007). Lastly, A. marensis was sampled (at 0 m depth) and genomically annotated by Seo and Lee (2010).…”

Altererythrobacter marinus sp. nov., isolated from deep seawater

“…The genus Altererythrobacter was first proposed by Kwon et al (2007) and comprises three species with validly published names: Altererythrobacter epoxidivorans (Kwon et al, 2007), A. luteolus (Yoon et al, 2005b;Kwon et al, 2007) and A. indicus (Kumar et al, 2008). Members of the genus Altererythrobacter are non-motile and do not contain bacteriochlorophyll a (BChl a) as a photosynthetic pigment.…”